Herbicidal activity of formulated sorgoleone, a natural product of sorghum root exudate.

BACKGROUND: The allelochemical sorgoleone, a major component of the hydrophobic root exudates of Sorghum bicolor, was formulated as a wettable powder [4.6 WP] and evaluated as a natural herbicide on several weed and crop species under different growth conditions. RESULTS: Formulated sorgoleone [4.6 WP] suppressed germination and shoot growth of weeds, with broadleaf species showing greater susceptibility than grass weed species. Germination and growth of broadleaf weed species were completely suppressed (100%) at 0.2 g a.i. L(-1) sorgoleone in a growth chamber study. Post-emergence applications of the wettable formulation of sorgoleone [4.6 WP] inhibited 20-25% higher growth of weeds than pre-emergence applications under greenhouse conditions. Broadleaf weeds were more susceptible than grass species to both methods of application. In all studies, growth was suppressed in more than 90% of the broadleaf weeds and two species, in particular, Rumex japonicus and Plantago asiatica, were completely suppressed at 0.4 kg a.i. ha(-1) sorgoleone. The crop species, on the other hand, were much more tolerant to sorgoleone, with 30% inhibition, at most, at the highest rate of 0.4 kg a.i. ha(-1) sorgoleone. CONCLUSION: The results of this study reveal that sorgoleone, after formulation as a WP, is more effective in inhibiting weed growth, and crop species are tolerant to it. The strong weed suppressive ability of formulated sorgoleone therefore offers interesting possibilities as an effective natural environment-friendly approach for weed management.

Effects of auxins on sorgoleone accumulation and genes for sorgoleone biosynthesis in sorghum roots.

Sorgoleone is a major component of the hydrophobic root exudate of Sorghum bicolor and is of particular interest to plant chemical ecology as well as agriculture. Sorgoleone was evaluated in this study to observe the expression levels of genes involved in its biosynthesis in response to auxins. Sorgoleone content varied widely according to the duration of application and the concentrations of the auxins. When the application time was increased, the sorgoleone content increased accordingly for all concentrations of IBA (1, 3, and 5 mg/L) and at 1 mg/L for both IAA and NAA. In this study, five different sorgoleone biosynthetic genes were observed, namely DES2, DES3, ARS1, ARS2, and OMT3, which are upregulated in response to IAA, IBA, and NAA. Transcript accumulation was apparent for all genes, but particularly for DES2, which increased up to 475-fold and 180-fold following 72 h exposure to NAA and IBA, respectively, compared to no treatment.

Enhancing sorgoleone levels in grain sorghum root exudates.

Sorgoleone, found in the root exudates of sorghum [(Sorghum bicolor (L.) Moench], has been a subject of continued research. Sorgoleone production in grain sorghum roots was investigated under different growth conditions. Methanol was the most effective solvent for extracting sorgoleone from grain sorghum roots. Sorgoleone production is high in young developing plants. The maximum concentration (microg mg(-1) root dry weight) was produced in 5-d-old seedlings; beyond this age, production declined. However, considering both root weight and sorgoleone content per seedling, 10-d-old seedlings had the highest total amounts (microg). Compared with the control, sorgoleone content increased 6.1, 8.6, and 14.2 times when sorghum seeds were treated with auxins, Hoagland solution, and a combination of auxins and Hoagland solution, respectively. Among the innate immunity response elicitors, cellulose (an elicitor of plant origin) stimulated higher sorgoleone production than the others, and it produced 6.2 times more sorgoleone than the control. Combined treatment of sorghum seeds with half strength Hoagland solution and 5 microg ml(-1) of IBA significantly increased both root growth and sorgoleone content in sorghum seedlings.