Chemical profile, antituberculosis, DSC, and molecular docking studies of Mentha longifolia essential oil.

The volatile profile of Mentha longifolia was observed using GC-MS, GC-FID, FT-IR and 13CNMR. Twenty-two constituents were found to be present in this essential oil, accounting 96.04 (%) of total essential oil and oxegenated monoterpenes were major class of compounds. The key constituents of this essential oil were pieritenone oxide (45.9%), piperitone (17.5%), beta caryophyllene (10.2%), and Germacrene D (5.0%). FT-IR showed peak at 1669 and 1707 cm-1 which may be due to the presence of carbonyl groups. Among the tested compounds, Germacrene D showed highest binding affinity value of -6.8 kcal mol-1 and a pKi value of 6.01. The dsc studies revealed that boiling point of this EO is above 200 °C. Microplate Alamar Blue assay (MABA) was carried out for the assessment of antimycobacterial activity using isoniazid and nicotinic as reference compound and oil was found to be active within conc. range of 0.8-1.6 µg/mL against mycobacterium tuberculosis, hence can act as a potential candidate against antituberculosis.

Isolation, characterisation, antifungal activity and validated UPLC/MS/MS method for quantification of novel compound from Artemisia tournefortiana Reichb.

Investigation into the chemical diversity of Artemisia tournefortiana resulted in isolation of one novel compound named tournefortin A and two known artemetin and tournefortin B bioactive compounds. Tournefortin B is first time obtained from natural source. The structure of all the isolated compounds were elucidated by detailed 1D and 2D NMR including HSQC, HMBC, 1H-1HOSY and NOESY spectroscopic techniques. Minimum inhibitory concentration (MIC) of all the tested compounds against tested fungal strains lies between 0.4 and 6.4 µg/mL and lowest MIC of 0.4 µg/mL of compound tournefortin A was found against Alternaria alternate. All the isolated compounds were quantified through UPLC/MS/MS and the developed method will serve as a first fingerprint method for the rapid determination of these phytomolecules in various plant extracts. Tournefortin B was found to be present in higher concentration. The higher antifungal effect of the isolated compounds suggests that this plant could act as potential source of antimicrobial agents.[Formula: see text].

Volatile composition, antibacterial and antioxidant activities of artemisia tournefortiana Reichb. from Kashmir, India.

The importance of the present study is to find out the phytochemical profile of essential oil (EO) of Artemisia tournefortiana Reichb. and its biological evaluation. Nineteen volatile constituents were identified from EO representing 93.47% of total oil composition. Oxygenated monoterpenes (54.46%) were found to be dominant over other class of compounds. cis-spiroether (47.66%), Z-ß-farnesene (22.83%), trans-nerolidol (3.89%) and camphor (3.80%) were found to be the major constituents. cis-spiroether is first time identified in this region. Antibacterial effects were observed against both gram-positive and gram-negative bacteria with maximum zone of inhibition (32 mm) against Staphylococcus aureus and minimum inhibitory concentration (MIC) of all tested strains were found in the range of 1.6-3.4 µg/mL. Hence the antimicrobial effect of this plant EO obtained from the natural source could be utilised in order to overcome the problem of microbial drug resistance. The EO showed moderate antioxidant effect through DPPH assay with IC50 value of 56.2 µg/mL. The significant antioxidant activity can be attributed to the presence of various conjugated secondary metabolites, phenolics and hydroxyl group bearing constituents present in the essential oil.[Figure: see text].

Chemical composition, antioxidant and cytotoxic activity of Artemisia gmelinii essential oil growing wild in Kashmir valley.

The present study was carried to observe the phytochemical profile of aromatic constituents of Artemisia gmelinni essential oil using GC-FID, GC-MS and 13C NMR and to evalute anticancer and antioxidant activities. Twenty chemical constituents were detected from EO accounting 92.05% of total oil composition. Oxygenated monoterpenes (73.64%) were dominant class of compounds. The major constituents are isoascaridol (29.70%), alpha-terpinolene (25.37%), phellandrene (9.26%) and ascaridole (4.17%). Ascaridole and isoascaridole are first time identified to be the constituents of this essential oil. The essential oil effectively inhibit the growth of cancer cells and showed maximum anti-proliferative activity at 125µg/mL concentration, but highest inhibition in cell growth was found in A-549 cell line. Our study revealed that EO was effective in restricting the migration of A-549 cells up to 15% than control at 125 µg/mL concentration. The essential oil also showed moderate antioxidant activity.