Case report: Quadruple primary malignant neoplasms including esophageal, ureteral, and lung in an elderly male.

Multiple primary malignant neoplasms (MPMNs) are defined as multiple tumors with different pathogenic origins. MPMNs are rare, but the morbidity rate is on the rise. With the development of anti-tumor treatments, such as targeted therapy and immunotherapy, the overall survival of cancer patients has been significantly prolonged, leading to an increased number of patients with MPMNs. A crucial aspect of MPMNs management is deciding how to schedule further treatments according to individual tumor risk. This process involves a multidisciplinary physician team to ensure favorable outcomes. Herein we report a 60-year-old male who developed four different malignancies, including esophageal squamous cell carcinoma, upper urinary tract urothelial carcinoma, mediastinal small cell lung cancer, and left lung squamous cell carcinoma over 20 years and received appropriate treatment of each cancer with long survival.

ITGBL1 promotes gastric cancer cell proliferation and invasion via Akt signal pathway.

Integrin beta- like 1 (ITGBL1), an extracellular matrix protein, plays an oncogenic role in diverse forms of cancers. To this end, we examined the importance of ITGBL1 in gastric cancer (GC). The upregulated expression of ITGBL1 in GC was associated with a poor prognosis. Moreover, upregulation of ITGBL1 enhanced cell mobility while silencing it exerted an opposite effect. Up-regulation of ITGBL1 significantly promoted phosphorylation of Akt, decreased the ratio of phosphorylated Akt in AGS/ITGBL1-shRNA and N87/ITGBL1-shRNA cells, enhanced cell mobility and proliferation. Silencing ITGBL1 had an opposite effect on Akt phosphorylation, cell mobility, and proliferation. These findings show that ITGBL1 regulates mobility and proliferation of GC likely through activation of Akt signaling.

Effect of contrast enhancement in delineating GTV and constructing IGTV of thoracic oesophageal cancer based on 4D-CT scans.

OBJECTIVE: To investigate the effect of contrast enhancement on delineating the gross tumour volumes (GTVs) of different respiratory phases and constructing the corresponding internal GTVs (IGTVs) of primary thoracic oesophageal cancer based on four-dimensional computed tomography (4D-CT) scans. METHODS: Forty-five patients with upper (14 cases), middle (16 cases), or lower (15 cases) thoracic oesophageal cancer sequentially underwent conventional plain and contrast-enhanced 4D-CT scans during free breathing. First, the GTVs were delineated on plain 4D-CT, and the corresponding IGTVs were constructed by a physician. Then the GTVs were delineated on contrast-enhanced 4D-CT images, and the corresponding IGTVs were constructed by the same physician using the same standards. RESULTS: The coefficient of variation for the target volume delineated on contrast-enhanced 4D-CT images was constantly smaller than that for plain 4D-CT images. The length of the GTVs along the z axis, as well as the volumes of the GTVs that were delineated and the IGTVs that were constructed, did not change between contrast-enhanced and plain 4D-CT images in patients with upper or lower thoracic oesophageal cancer (P>0.05), but showed significant differences in patients with middle thoracic oesophageal cancer (P<0.05). CONCLUSIONS: Contrast-enhanced 4D-CT scans can reduce the error of target volume delineation and be used to construct a more accurate internal target volume in patients with middle thoracic oesophageal cancer, however, whether GTV delineation or IGTV construction for patients with upper or lower thoracic oesophageal cancer, no significant benefit was found from contrast-enhanced 4D-CT scan.

Changes in tumour volume and motion during radiotherapy for thoracic oesophageal cancer.

BACKGROUND AND PURPOSE: Variations of target volume and position were important factors in correction of radiotherapy planning. The purpose was to investigate the changes in volume and motion of oesophageal cancer during radiotherapy using four-dimensional computed tomography (4D-CT). METHODS AND MATERIALS: In total, 109 enhanced 4D-CT data sets were acquired for 38 patients throughout treatment. Gross tumour volumes (GTVs) were outlined on each data set. Variations in volume, motion, and position were calculated for GTV and internal GTV (IGTV) during treatment. RESULTS: GTV (25%, P<0.01) and IGTV (27%, P<0.01) had decreased significantly when measured at the twentieth fraction. Larger intrafractional GTV centre shifts (P<0.01) were observed in the superior-inferior direction (median value of 3.1mm) compared with the right-left and anterior-posterior directions (1.6mm and 1.4mm, respectively). The interfractional shift of the IGTV centre was not significant during radiotherapy. The overlap ratios of the targets decreased for both GTV and IGTV during treatment. CONCLUSIONS: Variations in GTV and IGTV centre shifts were not significant throughout treatment. However, tumour volume decreased significantly by the twentieth fraction. Finally, changes in oesophageal tumour volume and motion may decrease the overlap ratio for GTV and IGTV during radiotherapy.

[Comparison of the displacements of peripheral lung cancer based on 4D CT scan and 3D CT scan assisted with active breathing control].

OBJECTIVE: To compare the position, displacement, degree of inclusion (DI) and matching index (MI) of the gross tumor volume (GTV) for peripheral lung cancer based on 4-dimensional CT (4D CT) and 3-dimensional CT (3D CT) assisted with active breathing control (ABC). METHODS: Eighteen patients with peripheral lung cancer underwent 4D CT simulation scan during free breathing and 3D CT simulation scans in end-inspiratory hold (CTEIH) and end-expiratory hold (CTEEH) in turn. The 4D CT images from each respiratory cycle were sorted into 10 phases. phase 0 was defined as end-inspiratory phase (CT0), and phase 50 was defined as end-expiratory phase (CT50). The GTVs were delineated separately on CT0, CT50, CTEIH and CTEEH images, and then GTV0, GTV50, GTVEIH and GTVEEH were constructed, respectively. RESULTS: The median distances between the centroids of GTV0 and GTVEIH, GTV50 and GTVEEH were 3.9 mm and 3.4 mm in all patients, 3.2 mm and 3.1 mm in the upper lobe group, and 5.0 mm and 4.7 mm in the lower lobe group, respectively. In the upper lobe group, the GTV0 and GTVEIH were 65.9% and 63.1%, and the median mutual DIs of GTV50 and GTVEEH were 67.5%, 63.1%, respectively. In the lower lobe group, the GTV0 and GTVEIH were 35.3% and 21.4%, and the median mutual DIs of GTV50 and GTVEEH were 27.8% and 24.8%, respectively. In the upper lobe group, the median MI of GTV0 and GTVEIH was 0.5, and the median MI of GTV50 and GTVEEH was 0.6. In the lower lobe group, the median MI of GTV0 and GTVEIH was 0.2, and the median MI of GTV50 and GTVEEH was 0.3. Whether in the upper or lower lobe groups, all the differences between displacements of centroid positions of GTVEIH and GTVEEH (ABC displacement) and GTV0 and GTV50 (4D displacement ) were <1 mm in three dimensional directions (all P>0.05). CONCLUSIONS: The target displacement of tumors based on 3D CT scanning in end-inspiratory hold and end-expiration hold can be used to construct internal target volume instead of that based on 4D CT scanning in extreme phase for peripheral lung cancers, but spatital mismatches of GTVs are obvious between extreme phases in 4D CT and corresponding phases in 3D CT assisted with ABC, especially for tumors of smaller volume and with larger motion amplitude.

Detection of interfraction displacement and volume variance during radiotherapy of primary thoracic esophageal cancer based on repeated four-dimensional CT scans.

BACKGROUND: To investigate the interfraction displacement and volume variation of primary thoracic esophagus carcinoma with enhanced four-dimensional computed tomography (4DCT) scanning during fractionated radiotherapy. METHODS: 4DCT data sets were acquired at the time of treatment simulation and every ten fraction for each of 32 patients throughout treatment. Scans were registered to baseline (simulation) 4DCT scans by using bony landmarks. The gross tumor volumes (GTVs) were delineated on each data set. Coordinates of the GTV centroids were acquired on each respiration phase. Distance between center of the GTV contour on the simulation scan and the centers on subsequent scans were used to assess interfraction displacement between fractions. Volumes were constructed using three approaches: The GTV delineated from the maximum intensity projection (MIP) was defined IGTVMIP, all 10 GTVs were combined to form IGTV10, GTVmean was the average of all 10 phases of each GTV. RESULTS: Interfraction displacement in left-right (LR), anterior-posterior (AP), superior-inferior (SI) directions and 3D vector were 0.13 ± 0.09 cm, 0.16 ± 0.12 cm, 0.34 ± 0.26 cm and 0.43 ± 0.24 cm, respectively between the tenth fraction and simulation 4DCT scan. 0.14 ± 0.09 cm, 0.19 ± 0.16 cm, 0.45 ± 0.43 cm and 0.56 ± 0.40 cm in LR, AP, SI and 3D vector respectively between the twentieth fraction and simulation 4DCT scan. Displacement in SI direction was larger than LR and AP directions during treatment. For distal esophageal cancer, increased interfraction displacements were observed in SI direction and 3D vector (P = 0.002 and P = 0.001, respectively) during radiotherapy. The volume of GTVmean, IGTVMIP, and IGTV10 decreased significantly at the twentieth fraction for middle (median: 34.01%, 33.09% and 28.71%, respectively) and distal (median: 22.76%, 25.27% and 23.96%, respectively) esophageal cancer, but for the upper third, no significant variation were observed during radiotherapy. CONCLUSIONS: Interfractional displacements in SI direction were larger than LR and AP directions. For distal location, significant changes were observed in SI direction and 3D vector during radiotherapy. For middle and distal locations, the best time to reset position should be selected at the twentieth fraction when the primary tumor target volume changed significantly, and it was preferable to guide target correction and planning modification.