Assuntos
Glicina , Cardiopatias/prevenção & controle , Proteínas de Vegetais Comestíveis/genética , Plantas Geneticamente Modificadas , Valina , Glicina/efeitos adversos , Cardiopatias/diagnóstico , Cardiopatias/epidemiologia , Humanos , Ligação de Hidrogênio , Proteínas de Vegetais Comestíveis/efeitos adversos , Plantas Geneticamente Modificadas/efeitos adversos , Medição de Risco , Fatores de Risco , Valina/efeitos adversosAssuntos
Doenças Cardiovasculares/prevenção & controle , Dieta Saudável , Exercício Físico , Doenças Neurodegenerativas/prevenção & controle , Prevenção Primária/métodos , Comportamento de Redução do Risco , Doenças Cardiovasculares/diagnóstico , Doenças Cardiovasculares/epidemiologia , Humanos , Doenças Neurodegenerativas/diagnóstico , Doenças Neurodegenerativas/epidemiologia , Fatores de RiscoRESUMO
Gene synthesis is an emerging field which has widespread implications in synthetic biology and molecular biology. The field is constantly evolving which has led to key advances in oligonucleotide synthesis and gene synthesis technologies, with simplicity, cost effectiveness and high throughput. The miniaturization, multiplexing, microfluidic processing and the integrated microchip engineering will drive down cost and increase productivity without compromising DNA synthesis fidelity, whereas the gigantic amount of genome information provides infinite source of DNA elements and genes as raw material for synthetic biology. This article describes some of the recent patents on oligonucleotide synthesis and gene synthesis.
Assuntos
Genes Sintéticos/genética , Oligonucleotídeos/síntese química , Patentes como Assunto , Técnicas Analíticas Microfluídicas , Oligonucleotídeos/química , Compostos Organofosforados/química , Reação em Cadeia da Polimerase , Enxofre/químicaRESUMO
SNX-2112 is an Hsp90 inhibitor which is currently undergoing multiple phase 1 clinical trials; however, its mechanism of action needs to be further elaborated. Here we investigated the effects of SNX-2112 in A-375 cells. SNX-2112 induced the degradation of multiple Hsp90 client proteins, activated both the mitochondrial-mediated and death receptor-mediated apoptotic pathways, downregulated Bcl-2 and Bcl-xL, upregulated Bid, cleaved caspase-9, caspase-7, caspase-3 and PARP, and activated caspase-8. The general caspase inhibitor, z-VAD-fmk, did not completely abolish SNX-2112-induced cell death. SNX-2112 induced autophagy in a time- and dose-dependent manner via Akt/mTOR/p70S6K inhibition. SNX-2112 induces significant apoptosis and autophagy in human melanoma A-375 cells, and may be an effective targeted therapy agent.
