Efficient degradation of sulfadiazine by UV-triggered electron transfer on oxalic acid-functionalized corn straw biochar for activating peroxyacetic acid: Performance, mechanism, and theoretical calculation.

A novel UV/oxalic acid functionalized corn straw biochar (OCBC)/peroxyacetic acid (PAA) system was built to degrade sulfadiazine from waters. 94.7 % of SDZ was removed within 30 min by UV/OCBC/PAA. The abundant surface functional groups and persistent free radicals (PFRs) on OCBC were responsible for these performances. Cyclic voltammetry (CV) and other characterization analysis revealed, under UV irradiation, the addition of OCBC served as electron donor, which might promote the reaction of electrons with PAA. The quenching and electron paramagnetic resonance (EPR) tests indicated that R-O•, 1O2 and •OH were generated. Theoretical calculations indicated sulfonamide bridge was vulnerable under the attacks of reactive species. In addition, high removal effect achieved by 5 reuse cycles and different real waters also suggested the sustainability of UV/OCBC/PAA. Overall, this study provided a feasible approach to remove SDZ with high mineralization efficiency, in addition to a potential strategy for resource utilization of corn straw.

Genetic characterization of Tibetan pigs adapted to high altitude under natural selection based on a large whole-genome dataset.

The Qinghai-Tibet Plateau is a valuable genetic resource pool, and the high-altitude adaptation of Tibetan pigs is a classic example of the adaptive evolution of domestic animals. Here, we report the presence of Darwinian positive selection signatures in Tibetan pigs (TBPs) using 348 genome-wide datasets (127 whole-genome sequence datasets (WGSs) and 221 whole-genome single-nucleotide polymorphism (SNP) chip datasets). We characterized a high-confidence list of genetic signatures related response to high-altitude adaptation in Tibetan pigs, including 4,598 candidate SNPs and 131 candidate genes. Functional annotation and enrichment analysis revealed that 131 candidate genes are related to multiple systems and organs in Tibetan pigs. Notably, eight of the top ten novel genes, RALB, NBEA, LIFR, CLEC17A, PRIM2, CDH7, GK5 and FAM83B, were highlighted and associated with improved adaptive heart functions in Tibetan pigs high-altitude adaptation. Moreover, genome-wide association analysis revealed that 29 SNPs were involved in 13 candidate genes associated with at least one adaptive trait. In particular, among the top ten candidate genes, CLEC17A is related to a reduction in hemoglobin (HGB) in Tibetan pigs. Overall, our study provides a robust SNP/gene list involving genetic adaptation for Tibetan pig high-altitude adaptation, and it will be a valuable resource for future Tibetan pig studies.

Sex-biased regulatory changes in the placenta of native highlanders contribute to adaptive fetal development.

Compared with lowlander migrants, native Tibetans have a higher reproductive success at high altitude though the underlying mechanism remains unclear. Here, we compared the transcriptome and histology of full-term placentas between native Tibetans and Han migrants. We found that the placental trophoblast shows the largest expression divergence between Tibetans and Han, and Tibetans show decreased immune response and endoplasmic reticulum stress. Remarkably, we detected a sex-biased expression divergence, where the male-infant placentas show a greater between-population difference than the female-infant placentas. The umbilical cord plays a key role in the sex-biased expression divergence, which is associated with the higher birth weight of the male newborns of Tibetans. We also identified adaptive histological changes in the male-infant placentas of Tibetans, including larger umbilical artery wall and umbilical artery intima and media, and fewer syncytial knots. These findings provide valuable insights into the sex-biased adaptation of human populations, with significant implications for medical and genetic studies of human reproduction.

Different responses of abundant and rare bacterial composition to groundwater depth and reduced nitrogen application in summer maize field.

Introduction: It is well known that reduced nitrogen application and groundwater depth can change soil microbial communities, but the associated difference in the response of abundant and rare bacterial composition to these local environmental changes remains unclear. Methods: In this study a lysimeter experiment was carried out to examine the impact of reduced nitrogen and groundwater depth on the composition of abundant and rare bacteria. Results and discussion: Our results demonstrated that the summer maize field soil species composition of rare bacterial sub-communities was significantly regulated by reduced nitrogen application, groundwater depth change and their interactions. However, only reduced nitrogen application had a significant influence on the species composition of abundant bacterial sub-communities. The structural equation model (SEM) indicated that reduced nitrogen application and groundwater depth change also could indirectly regulate the species composition of abundant and rare bacteria by altering soil attributes. The changes in soil pH and TSN had the most significant effects on the community composition of abundant and rare bacteria, respectively. More importantly, rare bacterial sub-communities were more sensitive to the changes in nitrogen input, groundwater depth and soil factors. Collectively, our study first demonstrated that abundant and rare microbial sub-communities responded differently to reduced nitrogen application and groundwater depth change. This study highlights that summer maize farmland production management should take nitrogen input and groundwater depth into consideration to maintain the compositional stability of soil rare microbial sub-communities.

Genetic and cultural adaptations underlie the establishment of dairy pastoralism in the Tibetan Plateau.

BACKGROUND: Domestication and introduction of dairy animals facilitated the permanent human occupation of the Tibetan Plateau. Yet the history of dairy pastoralism in the Tibetan Plateau remains poorly understood. Little is known how Tibetans adapted to milk and dairy products. RESULTS: We integrated archeological evidence and genetic analysis to show the picture that the dairy ruminants, together with dogs, were introduced from West Eurasia into the Tibetan Plateau since ~ 3600 years ago. The genetic admixture between the exotic and indigenous dogs enriched the candidate lactase persistence (LP) allele 10974A > G of West Eurasian origin in Tibetan dogs. In vitro experiments demonstrate that - 13838G > A functions as a LP allele in Tibetans. Unlike multiple LP alleles presenting selective signatures in West Eurasians and South Asians, the de novo origin of Tibetan-specific LP allele - 13838G > A with low frequency (~ 6-7%) and absence of selection corresponds - 13910C > T in pastoralists across eastern Eurasia steppe. CONCLUSIONS: Results depict a novel scenario of genetic and cultural adaptations to diet and expand current understanding of the establishment of dairy pastoralism in the Tibetan Plateau.

Differences in Cadmium Uptake and Accumulation in Seedlings of Wheat Varieties with Low- and High-Grain Cadmium Accumulation under Different Drought Stresses.

Cadmium (Cd) and drought, as abiotic stresses, have long been significant challenges for crop growth and agricultural production. However, there have been relatively few studies conducted on the effects of drought stress on Cd uptake, especially regarding the differences in Cd uptake characterization in varieties with varying Cd accumulation under different drought stress. To investigate the effects of drought conditions on Cd uptake by wheat in different genotypes under specific background levels of Cd pollution, we validated the differences in root absorption characteristics of low- (YM) and high-grain Cd accumulating wheat genotypes (XM) using non-invasive micro-test technology, and we conducted a hydroponic experiment on the Cd addition and different drought levels in a climate-controlled chamber. The biomass, root morphology, Cd uptake, and accumulation were determined under Cd (100 µmol L-1) and different drought levels of 0% (0 MPa), 5% (-0.100 Mpa), 10% (-0.200 Mpa), and 15% (-0.388 Mpa) simulated by polyethylene glycol (PEG-6000). We found that the simultaneous exposure to Cd and drought had a suppressive effect on the total root lengths, root surface areas, and root volumes of XM and YM, albeit with distinct patterns of variation. As the concentration of PEG-6000 increased, the Cd concentrations and the amount of Cd accumulated in the roots and shoots of XM and YM decreased. Specifically, the Cd concentration in the roots exhibited a reduction ranging from 12.51% to 66.90%, while the Cd concentration in the shoots experienced an even greater decrease of 50.46% to 80.57%. The PEG-6000 concentration was significantly negatively correlated (p < 0.001) with Cd concentration of roots and shoots and Cd accumulation in roots, shoots, and the whole plants and significantly negatively correlated (p < 0.05) with the total length, surface area, and volume of roots. This study confirms that drought stress (5% PEG-6000) can decrease the uptake and accumulation of Cd in wheat seedlings without significant inhibition of biomass, and the change of root morphology (root length) and the decrease of Cd concentration in roots may be the main direct pathways for achieving these effects under drought stress. This research provides a new perspective and idea for water management in Cd-contaminated farmland.

Deep phenotyping of 11,880 highlanders reveals novel adaptive traits in native Tibetans.

Tibetans are the ideal population to study genetic adaptation in extreme environments. Here, we performed systematic phenotyping of 11,880 highlanders, covering 133 quantitative traits of 13 organ systems. We provided a comprehensive phenotypic atlas by comparing altitude adaptation and altitude acclimatization. We found the differences between adaptation and acclimatization are quantitative rather than qualitative, with a whole-system "blunted effect" seen in the adapted Tibetans. We characterized twelve different functional changes between adaptation and acclimatization. More importantly, we established a landscape of adaptive phenotypes of indigenous Tibetans, including 45 newly identified Tibetan adaptation-nominated traits, involving specific changes of Tibetans in internal organ state, metabolism, eye morphology, and skin pigmentation. In addition, we observed a sex-biased pattern between altitude acclimatization and adaptation. The generated atlas of phenotypic landscape provides new insights into understanding of human adaptation to high-altitude environments, and it serves as a valuable blueprint for future medical and physiological studies.

Polygenic adaptation leads to a higher reproductive fitness of native Tibetans at high altitude.

The adaptation of Tibetans to high-altitude environments has been studied extensively. However, the direct assessment of evolutionary adaptation, i.e., the reproductive fitness of Tibetans and its genetic basis, remains elusive. Here, we conduct systematic phenotyping and genome-wide association analysis of 2,252 mother-newborn pairs of indigenous Tibetans, covering 12 reproductive traits and 76 maternal physiological traits. Compared with the lowland immigrants living at high altitudes, indigenous Tibetans show better reproductive outcomes, reflected by their lower abortion rate, higher birth weight, and better fetal development. The results of genome-wide association analyses indicate a polygenic adaptation of reproduction in Tibetans, attributed to the genomic backgrounds of both the mothers and the newborns. Furthermore, the EPAS1-edited mice display higher reproductive fitness under chronic hypoxia, mirroring the situation in Tibetans. Collectively, these results shed new light on the phenotypic pattern and the genetic mechanism of human reproductive fitness in extreme environments.

High Arterial Oxygen Saturation in the Acclimatized Lowlanders Living at High Altitude.

Blood oxygen saturation (SpO2) is a key indicator of oxygen availability in the body. It is known that a low SpO2 at high altitude is associated with morbidity and mortality risks due to physiological hypoxemia. Previously, it was proposed that the lowlander immigrants living at high altitude should have a lower SpO2 level compared to the highlander natives, but this proposal has not been rigorously tested due to the lack of data from the lowlander immigrants living at high altitude. In this study, we compared arterial oxygen saturation of 5929 Tibetan natives and 1034 Han Chinese immigrants living at altitudes ranging from 1120 m to 5020 m. Unexpectedly, the Han immigrants had a higher SpO2 than the Tibetan natives at the same high altitudes. At the same time, there is a higher prevalence of chronic mountain sickness in Han than in Tibetans at the same altitude. This result suggests that the relatively higher SpO2 level of the acclimatized Han is associated with a physiological cost, and the SpO2 level of Tibetans tends to be sub-optimal. Consequently, SpO2 alone is not a robust indicator of physiological performance at high altitude. Supplementary Information: The online version contains supplementary material available at 10.1007/s43657-023-00117-x.

One-pot preparation of layered double oxides-engineered biochar for the sustained removal of tetracycline in water.

Tetracycline (TC) and sugarcane bagasse had both exerted enormous strain on environmental security. In this work, new composite adsorbent designed by impregnating bio-waste bagasse with magnesium-aluminum layered double oxides (BC-MA) was innovatively brought forward for TC removal. Benefiting from the abundant adsorption sites supplied by developed pores structure (0.308 cm3·g-1), enlarged surface area (256.8 m2·g-1) and reinforced functional groups, the maximum adsorption amount of BC-MA for TC reached 250.6 mg g-1. Moreover, BC-MA displayed desirable adsorption capacity in diverse water environments coupled with excellent sustainable regeneration ability. The absorption process of TC by BC-MA was spontaneous and endothermic, and the pivotal rate-limiting stage pertained to intraparticle diffusion. The mechanisms proposed here mainly concerned π-π interactions, pore filling, complexation and hydrogen bonding. These findings suggested that the synthesis of modified biochar from bagasse would offer new opportunities for simultaneous waste resource reuse and water pollution control.

Large-scale genome sequencing redefines the genetic footprints of high-altitude adaptation in Tibetans.

BACKGROUND: Tibetans are genetically adapted to high-altitude environments. Though many studies have been conducted, the genetic basis of the adaptation remains elusive due to the poor reproducibility for detecting selective signatures in the Tibetan genomes. RESULTS: Here, we present whole-genome sequencing (WGS) data of 1001 indigenous Tibetans, covering the major populated areas of the Qinghai-Tibetan Plateau in China. We identify 35 million variants, and more than one-third of them are novel variants. Utilizing the large-scale WGS data, we construct a comprehensive map of allele frequency and linkage disequilibrium and provide a population-specific genome reference panel, referred to as 1KTGP. Moreover, with the use of a combined approach, we redefine the signatures of Darwinian-positive selection in the Tibetan genomes, and we characterize a high-confidence list of 4320 variants and 192 genes that have undergone selection in Tibetans. In particular, we discover four new genes, TMEM132C, ATP13A3, SANBR, and KHDRBS2, with strong signals of selection, and they may account for the adaptation of cardio-pulmonary functions in Tibetans. Functional annotation and enrichment analysis indicate that the 192 genes with selective signatures are likely involved in multiple organs and physiological systems, suggesting polygenic and pleiotropic effects. CONCLUSIONS: Overall, the large-scale Tibetan WGS data and the identified adaptive variants/genes can serve as a valuable resource for future genetic and medical studies of high-altitude populations.

Lightweight SM-YOLOv5 Tomato Fruit Detection Algorithm for Plant Factory.

Due to their rapid development and wide application in modern agriculture, robots, mobile terminals, and intelligent devices have become vital technologies and fundamental research topics for the development of intelligent and precision agriculture. Accurate and efficient target detection technology is required for mobile inspection terminals, picking robots, and intelligent sorting equipment in tomato production and management in plant factories. However, due to the limitations of computer power, storage capacity, and the complexity of the plant factory (PF) environment, the precision of small-target detection for tomatoes in real-world applications is inadequate. Therefore, we propose an improved Small MobileNet YOLOv5 (SM-YOLOv5) detection algorithm and model based on YOLOv5 for target detection by tomato-picking robots in plant factories. Firstly, MobileNetV3-Large was used as the backbone network to make the model structure lightweight and improve its running performance. Secondly, a small-target detection layer was added to improve the accuracy of small-target detection for tomatoes. The constructed PF tomato dataset was used for training. Compared with the YOLOv5 baseline model, the mAP of the improved SM-YOLOv5 model was increased by 1.4%, reaching 98.8%. The model size was only 6.33 MB, which was 42.48% that of YOLOv5, and it required only 7.6 GFLOPs, which was half that required by YOLOv5. The experiment showed that the improved SM-YOLOv5 model had a precision of 97.8% and a recall rate of 96.7%. The model is lightweight and has excellent detection performance, and so it can meet the real-time detection requirements of tomato-picking robots in plant factories.

Effects of nitrogen application on winter wheat growth, water use, and yield under different shallow groundwater depths.

Shallow groundwater plays a vital role in physiology morphological attributes, water use, and yield production of winter wheat, but little is known of its interaction with nitrogen (N) application. We aimed to explore the effects of N fertilization rate and shallow groundwater table depth (WTD) on winter wheat growth attributes, yield, and water use. Experiments were carried out in micro-lysimeters at WTD of 0.6, 0.9, 1.2, and 1.5 m with 0, 150, 240, and 300 kg/ha N application levels for the winter wheat (Triticum aestivum L.). The results showed that there was an optimum groundwater table depth (Op-wtd), in which the growth attributes, groundwater consumption (GC), yield, and water use efficiency (WUE) under each N application rate were maximum, and the Op-wtd decreased with the increase in N application. The Op-wtd corresponding to the higher velocity of groundwater consumption (Gv) appeared at the late jointing stage, which was significantly higher than other WTD treatments under the same N fertilization. WTD significantly affected the Gv during the seeding to the regreening stage and maturity stage; the interaction of N application, WTD, and N application was significant from the jointing to the filling stage. The GC, leaf area index (LAI), and yield increased with an increase of N application at 0.6-0.9-m depth-for example, the yield and the WUE of the NF300 treatment with 0.6-m depth were significantly higher than those of the NF150-NF240 treatment at 20.51%, and 14.81%, respectively. At 1.2-1.5-m depth, the N application amount exceeding 150-240 kg/ha was not conducive to wheat growth, groundwater use, grain yield, and WUE. The yield and the WUE of 150-kg/ha treatment were 15.02% and 10.67% higher than those of 240-300-kg/ha treatment at 1.2-m depth significantly. The optimum N application rate corresponding to yield indicated a tendency to decrease with the WTD increase. Considering the winter wheat growth attributes, GC, yield, and WUE, application of 150-240 kg/ha N was recommended in our experiment.

Spatiotemporal distribution, sources apportionment and ecological risks of PAHs: a study in the Wuhan section of the Yangtze River.

This study investigated the sources, contamination and ecological risks of polycyclic aromatic hydrocarbons (PAHs) based on their spatiotemporal distribution in aquatic environment in the Wuhan section of the Yangtze River (WYR). The fugacity ratio evaluation indicated that sediment was secondary release sources of two- and three-ring PAHs and sinks of four- and five-ring PAHs. The total concentrations of PAHs (Σ16PAHs) ranged from 2.51 to 102.5 ng/L in water with the dominant contribution of 47.8% by two-ring PAHs. Σ16PAHs in sediments varied from 5.90 to 2926 ng/g with the contribution of 35.4% by four-ring PAHs. The higher levels of PAHs occurred around developed industrial areas during the wet season, which was related to local industrial emissions and influenced by rainfall/runoff. Annual flux of Σ16PAHs was estimated of 28.77 t. The PMF model analysis revealed that petroleum and industrial emissions were the dominant sources in water accounting for 58.5% of the total pollution, although traffic emission was the main source for sediment accounting for 44.6%. Risk assessments showed that PAHs in water were at low risks, whereas about 44% of the sediments were identified as medium risks. Therefore, energy structure adjustment and further implement of regulation and monitoring are necessary to reduce PAH emissions.

Estimating Sources, Fluxes, and Ecological Risks of Antibiotics in the Wuhan Section of the Yangtze River, China: A Year-Long Investigation.

To our knowledge, ours is the first study to investigate the annual fluxes, environmental fate, and ecological risks of five categories of antibiotics from the Wuhan section of the Yangtze River (China). All the 24 antibiotics we tested for were detected in water, with total concentrations of 17.11-867.2 ng/L (mean: 63.69 ng/L), and 19 antibiotics were detected in sediment, at 0.02-287.7 ng/g (mean: 16.54 ng/g). Sulfonamides, amphenicols, and macrolides were the three most prominent antibiotic classes in water, and fluoroquinolones were the most prominent in sediment. Farming activities (animal husbandry and aquaculture) are proposed as the largest contributors to antibiotic pollution in the Wuhan section of the Yangtze River according to the Unmix model, followed by municipal wastewater and mixed sources. Higher pollution levels were observed downstream (combined discharge of these sources). Monthly monitoring data (12 months) were used to estimate antibiotic annual fluxes, with 101.5 t (uncertainty: 5.6%) in the Wuhan section of the Yangtze River. Risk assessments showed that erythromycin, clarithromycin, and azithromycin posed medium and high ecological risks and were found in 9%-35% and 1.8%-3.7% of all water samples, respectively; enrofloxacin, clarithromycin, azithromycin, florfenicol, and thiamphenicol posed medium resistance risks in 1.9%-16.7% of waters in the Wuhan section of the Yangtze River. Our results have filled data gaps on antibiotic sources, annual fluxes, and resistance risk in the Wuhan section of the Yangtze River and demonstrated the importance of further management of antibiotic use in the studied areas. Environ Toxicol Chem 2023;42:605-619. © 2022 SETAC.

Responses of the maize rhizosphere soil environment to drought-flood abrupt alternation stress.

Changes in the soil environment in the root zone will affect the growth, development and resistance of plants. The mechanism underlying the effect of drought and flood stress on rhizosphere bacterial diversity, soil metabolites and soil enzyme activity is not clear and needs further study. To analyze the dynamic changes in bacteria, metabolites and enzyme activities in the rhizosphere soil of maize under different drought-flood abrupt alternation (DFAA) stresses, the barrel test method was used to set up the 'sporadic light rain' to flooding (referring to trace rainfall to heavy rain) (DFAA1) group, 'continuous drought' to flooding (DFAA2) group and normal irrigation (CK) group from the jointing to the tassel flowering stage of maize. The results showed that Actinobacteria was the most dominant phylum in the two DFAA groups during the drought period and the rewatering period, and Proteobacteria was the most dominant phylum during the flooding period and the harvest period. The alpha diversity index of rhizosphere bacteria in the DFAA2 group during the flooding period was significantly lower than that in other stages, and the relative abundance of Chloroflexi was higher. The correlation analysis between the differential genera and soil metabolites of the two DFAA groups showed that the relative abundance of Paenibacillus in the DFAA1 group was higher during the drought period, and it was significantly positively correlated with the bioactive lipid metabolites. The differential SJA-15 bacterium was enriched in the DFAA2 group during the flooding period and were strongly correlated with biogenic amine metabolites. The relative abundances of Arthrobacter, Alphaproteobacteria and Brevibacillus in the DFAA2 group were higher compared with DFAA1 group from rewatering to harvest and were significantly positively correlated with hydrocarbon compounds and steroid hormone metabolites. The acid phosphatase activity of the DFAA1 group was significantly higher than that of the DFAA2 group during the flooding period. The study suggests that there is a yield compensation phenomenon in the conversion of 'continuous drought' to flooding compared with 'sporadic light rain', which is related to the improvement in the flooding tolerance of maize by the dominant bacteria Chloroflexi, bacterium SJA-15 and biogenic amine metabolites. These rhizosphere bacteria and soil metabolites may have the potential function of helping plants adapt to the DFAA environment. The study revealed the response of the maize rhizosphere soil environment to DFAA stress and provided new ideas for exploring the potential mechanism of crop yield compensation under DFAA.

Genetic adaptation of skin pigmentation in highland Tibetans.

Strong ultraviolet (UV) radiation at high altitude imposes a serious selective pressure, which may induce skin pigmentation adaptation of indigenous populations. We conducted skin pigmentation phenotyping and genome-wide analysis of Tibetans in order to understand the underlying mechanism of adaptation to UV radiation. We observe that Tibetans have darker baseline skin color compared with lowland Han Chinese, as well as an improved tanning ability, suggesting a two-level adaptation to boost their melanin production. A genome-wide search for the responsible genes identifies GNPAT showing strong signals of positive selection in Tibetans. An enhancer mutation (rs75356281) located in GNPAT intron 2 is enriched in Tibetans (58%) but rare in other world populations (0 to 18%). The adaptive allele of rs75356281 is associated with darker skin in Tibetans and, under UVB treatment, it displays higher enhancer activities compared with the wild-type allele in in vitro luciferase assays. Transcriptome analyses of gene-edited cells clearly show that with UVB treatment, the adaptive variant of GNPAT promotes melanin synthesis, likely through the interactions of CAT and ACAA1 in peroxisomes with other pigmentation genes, and they act synergistically, leading to an improved tanning ability in Tibetans for UV protection.

Effect of Organic Amendments in Soil on Physiological and Biochemical Attributes of Vachellia nilotica and Dalbergia sissoo under Saline Stress.

Vachellia nilotica (L.) P.J.H. Hurther & Mabb. and Dalbergia sissoo Roxb. are two of the most important multipurpose agroforestry tree species of the Indian sub-continent, but their growth in saline soils is greatly reduced. Recently, organic amendments have showed the potential to increase plant growth in salt-affected soils; however, the influence of using these amendments for growing the above-mentioned tree species under saline conditions is not yet quantified. Therefore, an experiment was devised to analyze the interactive effects of organic amendments in saline soils on the growth of V. nilotica and D. sissoo. Under controlled conditions, a pot experiment was conducted in sandy loam saline soils (EC = 20.5 dSm-1). Organic amendments from four diverse sources: farmyard manure (FYM), poultry manure (PM), slurry (SL), and farmyard manure biochar (FYMB) were employed in this study. At the harvesting time, data regarding morphological, physiological, ionic, and biochemical parameters were obtained. The current study results indicated that both tree species reacted differently, but positively, to diverse applied amendments. The maximum increment in total above-ground biomass, total below-ground biomass, and shoot length for V. nilotica (163.8%, 116.3%, and 68.2%, respectively) was observed in FYM amended soils, while the maximum increment for D. sissoo (128%, 86%, and 107%, respectively) was observed in FYMB amended soils, as compared to control. Minimum plant growth of both species was observed in untreated soils (saline soils). Likewise, the maximum potassium ion and minimum sodium ion concentrations were present in the root and shoots of plants (both species) treated with FYMB. The use of organic amendments resulted in decreased concentrations of malondialdehyde and hydrogen peroxide, and increased concentrations of antioxidant enzymes such as SOD, POD, and CAT. Moreover, higher photosynthetic rates and stomatal conductance were observed in the plants grown in amended soils. The findings of this study can be used to include the above-mentioned high-value tree species for future afforestation programs under saline conditions.

Efficient adsorptive removal of fluoroquinolone antibiotics from water by alkali and bimetallic salts co-hydrothermally modified sludge biochar.

Fluoroquinolones are one of most commonly used antibiotics for preventing and treating bacterial infections and their unsatisfactory removal by conventional wastewater treatment technology have aroused widespread attention. A novel adsorbent of KMSBC was the first time synthesized and tested to adsorb three typical fluoroquinolone antibiotics of CIP, NOR and OFL from water. The characterization analysis showed that KMSBC possessed the superior porous structure, abundant functional groups and greater graphitic degree. Together with kinetics, isotherms, thermodynamics and critical factors (e.g., biochar dose, reaction time/temperature, fluoroquinolone antibiotics concentration, pH, co-existing ionic strength and HA concentration) analysis suggested that pore filling, π-π conjugation, H-bonding and electrostatic interaction were the key mechanisms for fluoroquinolone antibiotics adsorption by KMSBC. KMSBC exhibited the optimum adsorption performance at pH = 5 despite the adsorbates. The maximum adsorption capacity of KMSBC for CIP, NOR and OFL were 49.9, 55.7 and 47.4 mg/g at 25 °C, respectively. Also, KMSBC exhibited the good magnetic sensitivity and stability with the leaching concentrations of Fe were far below than environmental limit (GB5749-2006) at various pH (from 3 to 12), ionic strength and HA concentrations. Additionally, KMSBC performed a stable sustainable adsorption performance in recycles by NaOH regeneration. Thus, KMSBC had the potential to be a promising adsorbent for fluoroquinolone antibiotics removal with favorable adsorption capacity, environmental security and easy regeneration performance.

Characterization and phylogenetic analysis of the mitochondrial genome of Holotrichia parallela (Coleoptera: Scarabaeidae: Melolonthinae).

Holotrichia parallela (Motschulsky, 1854) is an important pest for peanut, potato, and soybean in China, and it causes great economic losses. In this study, we sequenced and analyzed the complete mitochondrial genome (mitogenome) of H. parallela. This mitogenome was 16,975 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and two ribosomal RNA genes (rRNAs). Gene order was conserved and identical to most other previously sequenced Scarabaeidae. Most PCGs of H. parallela have the conventional start codons ATN, with the exception of cox1 (AAC). Except for three genes (cox1, cox2, and cox3) end with the incomplete stop codon T-, all other PCGs terminated with the stop codon TAA or TAG. Phylogenetic analysis positioned H. parallela in a well-supported clade with Rhopaea magnicornis, Polyphylla gracilicornis, and Melolontha hippocastani. The relationships (Dynastinae+(Cetoniinae+(Melolonthinae+(Rutelinae + Scarabaeinae)))) were supported in Scarabaeidae.