LncRNA PVT1 inhibits endothelial cells apoptosis in coronary heart disease through regulating MAPK1 expression via miR-532-3p.

Background: Coronary atherosclerotic heart disease (CAD) is an inflammatory vascular disease caused by atherosclerosis. Long non-coding RNAs are involved in the pathophysiological process of coronary heart disease. Here we investigated the regulatory effects of lncRNA PVT1 (PVT1) in human coronary artery endothelial cells (HCAECs).Methods: qRT-PCR and western blot were performed to detect gene and protein expressions. CCK-8, flow cytometry and wound healing assays were used to determine cell viability, apoptosis and migration of HCAECs. The binding relationship among miR-532-3p, PVT1 and MAPK1 was verified by dual luciferase reporter assay.Results: Overexpression of PVT1 markedly reduced cell apoptosis and increased cell proliferation and migration. However, miR-532-3p upregulation suppressed cell proliferation and migration and promoted apoptosis of HCAECs. PVT1 suppressed the expression of miR-532-3p via directly targeting miR-532-3p. And miR-532-3p overexpression abolished the effect of PVT1 upregulation on proliferation and apoptosis in HCAECs. Furthermore, MAPK1 acted as a target gene of miR-532-3p and miR-532-3p inhibited MAPK1 expression.Conclusion: PVT1 promoted MAPK1 expression by targeting miR-532-3p, thus inhibiting HCAECs apoptosis and promoting cell proliferation, suggesting PVT1 might have great potential as a therapeutic target for CAD.

Comparison of image quality and radiation doses of double lower extremities full-length photography by two X-ray exposure modes / 医疗卫生装备

Objective To compare two exposure modes on image quality and radiation dose when used for double lower extremities full-length photography so as to provide guidance for clinical application. Methods Totally 60 patients undergoing double lower extremities full-length photography were selected with sequential experiment and then divided into two groups randomly and equally,of whom,there were four ones with genu varum.In the two groups,one group applied the exposure mode of AEC,and the other involved in FIXED mode.Then the two groups were compared on image quality and radiation dose. Results The exposure radiation to the patient under FIXED mode was higher than that under AEC mode although the common patients had the image quality with no significant difference in the two groups, and FIXED mode resulted in higher image quality for the patients with genu varum than AEC mode.Conclusion Exposure modes have effects on image quality and exposure dose,and it's suggested that AEC mode be applied for common patients from the aspect of radiation protection while FIXED mode be used for the patients with genu varum. [Chinese Medical Equipment Journal, 2018,39(5):68-70,74]