RESUMO
OBJECTIVE: To abtain the genetic polymorphisms data of eleven Y-chromosome STR loci in Yunnan Han population, and to evaluate its forensic application values, which will provide the base data for forensic application. METHODS: DYS391, DYS389 I, DYS439, DYS389 II, DYS438, DYS437, DYS19, DYS392, DYS393, DYS390 and DYS385a/b loci were amplified by using Powerplex Y PCR amplification Kit and were analyzed by 310 genetic analyzer. 201 unrelated male individuals of Han population in Yunnan were investigated to determine the distributions of allele frequencies and haplotypes. RESULTS: 3, 5, 6, 8, 5, 4, 5, 8, 4, 7 alleles were found across the Y-STR loci except DYS38556 haplotypes were found across DYS385a/b locus. The minimum GD value was 0.4273 (DYS438), and the maximum was 0.9747 (DYS385a/b). 175 haplotypes were found, of which 154 were unique, 16 were found in 2 individuals, 5 were found in 3 individuals, and the observed haplotypes diversity value was 0.9984. CONCLUSION: The 11 Y-STR loci are highly polymorphic and are suitable for forensic personal identification and paternity testing in Yunnan Han population.
Assuntos
Povo Asiático/genética , Cromossomos Humanos Y/genética , Ciências Forenses/métodos , Polimorfismo Genético , Sequências de Repetição em Tandem , Alelos , China/etnologia , DNA/análise , Frequência do Gene , Genética Populacional , Haplótipos , Humanos , Masculino , Reação em Cadeia da Polimerase/métodos , Espermatozoides/citologiaRESUMO
Eleven Y-STRs loci including minimal haplotypes (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, and DYS385a,b) and two additional loci, namely DYS438 and DYS439 have been co-amplified in 100 healthy unrelated males of Chinese Yi minority ethnic group using the Y-PLEX 5 and Y-PLEX 6 kit, in order to investigate allele and haplotype frequencies of Yi population, evaluate their usefulness in forensic paternity testing and human identification, and enrich Chinese population genetic informational resources. Out of a total of 100 individuals 85 showed different haplotypes, while 8 haplotypes occurred more than once. The overall haplotype diversity for 11 Y-STRs loci was 0.9945.