RESUMO
PURPOSE: To explore the function of DREAM gene mediated by NF-kB signal pathway in the pathogenesis of osteosarcoma. METHODS: This study included 13 Sprague Dawley (SD) rats with osteosarcoma (treatment group) and 13 healthy rats (control group). NF-kB, DREAM and P105 mRNAs expression levels were determined using quantitative PCR (qPCR). The expression levels of NF-kB, DREAM and P105 proteins were evaluated using ELISA and western blot. Also, DREAM protein expression in rats was determined by immunofluorescence. RESULTS: NF-kB and DREAM levels in the treatment group were significantly higher than those in the control group (p<0.05). However, P105 mRNA expression level in the treatment group was significantly lower than in the control group (p<0.05). Results obtained from ELISA and western blot showed that NF-kB and DREAM expression levels in the treatment group were significantly higher than in the control group (p<0.05). NF-kB and DREAM levels in the treatment group were 4.3±0.12 µg/l and 6.8±0.21 µg/l, respectively. These levels in the control group were 0.96±0.11 µg/l and 1.25±0.18 µg/l, respectively. P105 expression level in the treatment group was 0.37±0.11 µg/l which was significantly lower than that in the control group (1.63±0.21 µg/l) (p<0.05). Immunofluorescence results showed that DREAM expression level was significantly higher in the treatment group (p<0.05). CONCLUSION: NF-kB signal pathway promoted the expression of DREAM gene and also promoted the pathogenesis and worsening of osteosarcoma.
