RESUMO
Polyspora tiantangensis (Theaceae) is an endangered woody plant in southwestern China. In this study, we assembled complete chloroplast (cp) genome of P. tiantangensis based on the Illumina reads. The cp genome of P. tiantangensis is 157,057 bp in length, including a large single-copy (LSC) region of 86,593 bp and a small single-copy (SSC) region of 18,284 bp, separated by two inverted repeat (IR) regions of 26,090 bp each. The cp genome encoded 132 genes including 87 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The GC content of cp genome of P. tiantangensis is 37.3%. A total of 68 SSRs were discovered. Phylogenetic analysis of cp genomes from 26 species of Theaceae revealed that all species of Polyspora formed one monophyletic clade and P. tiantangensis was closely related with its congeneric species P. longicarpa with 100% bootstrap value.
RESUMO
The first complete chloroplast genome of Wikstroemia chamaedaphne, a poisonous shrub with important medicinal value, is reported in this study. The plastome is a quadripartite circular shape with 173,042 bp in length. It consists of a large single-copy (LSC) region of 86,330 bp and a small single-copy (SSC) region of 2868 bp, separated by two inverted repeat (IR) regions of 41,922 bp each. The chloroplast genome contains 137 genes, including 91 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The GC content values in the whole cp genome, LSC region, SSC region, and IR region are 36.6%, 34.6%, 28.3%, and 38.9%, respectively. The corresponding numbers of mono-, di-, tri-, tetra- and penta-nucleotides SSRs were 73, 13, 9, 13, and 1. Phylogenetic study revealed that W. chamaedaphne and W. indica formed a monophyletic branch and having a close relationship with Stellera chamaejasme.
RESUMO
Lindera pulcherrima var. hemsleyana is an evergreen tree of the genus Lindera with important medicinal value. The complete chloroplast genome of L. pulcherrima var. hemsleyana is 152,710 bp in length, containing a LSC region of 93,751 bp, a SSC region of 18,813 bp, and a pair of inverted repeats (IRA and IRB) of 20,073 bp each. The genome encoded 128 genes, including 83 protein-coding genes, 8 ribosomal RNA genes, 36 transfer RNA genes, and one pseudogene (ycf1). Most of these genes occurred in a single copy, whereas 15 genes occurred in double copies, including all rRNA, 6 tRNA, and 5 protein-coding genes. The GC content of the whole genome, LSC, SSC, and IR regions is 39.2%, 38.0%, 33.9%, and 44.4%, respectively. A total of 90 SSRs were discovered, the numbers of mono-, di-, tri-, tetra-, penta-, and hexa-nucleotides SSRs were 66, 10, 4, 8, 1, and 1, respectively. Phylogenetic analysis of cp genomes from 24 species of Lauraceae revealed that cp genomes of L. pulcherrima var. hemsleyana, L. pulcherrima var. attenuata, and L. pulcherrima formed a monophyletic clade with 100% bootstrap value.
RESUMO
The complete chloroplast genome of Wikstroemia indica, a medicinal plant with a variety of medicinal functions in treatment for arthritis, whooping cough, cancer, and bacillosis, was generated by de novo assembly using whole genome next-generation sequencing. The plastome was a quadripartite circular with 151,731 bp in length; containing a large single-copy (LSC) region of 86,523 bp and a small single-copy (SSC) region of 12,384 bp; separated by 2 inverted repeat (IR) regions of 26,403 bp each. The chloroplast genome contained 124 genes, including 79 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The GC content in the whole cp genome, LSC region, SSC region, and IR region were 37.4, 34.9, 32.4, and 42.6%, respectively. The phylogenetic tree indicated that W. indica has a close relationship with Stellera chamaejasme with 100% support.