Comparative analysis of main agronomic traits and interrelationships in cultivation provenances of Pesudostellaria heterophylla from Guizhou province / 中国中药杂志

<p><b>OBJECTIVE</b>To provide guidance for selection and breeding of Pesudostellaria heterophylla, agronomic traits of 3 mainly cultivated form of P. heterophylla were observed and compared in Guizhou shibing.</p><p><b>METHOD</b>Thirteen agronomic traits of P. heterophylla from 3 cultivation form were measured and the traits were analyzed using multiple comparison, correlation analysis, multiple stepwise regression analysis and path analysis.</p><p><b>RESULT</b>The results showed that agronomic traits were different in 3 cultivation form, significant(P <0.05)or very significant correlated (P <0.01)among multiple agronomic traits, the width and the number of root, the first branch number, cleistogamous flowers, length and width of leaves, and the aboveground biomass were the main factors that affected the underground biomass, and the number of root, the aboveground biomass had a directly positive effect on the underground biomass. Meanwhile, whole length, length and width of leaves, cleistogamous flowers etc. had direct or indirect effect on the underground biomass.</p><p><b>CONCLUSION</b>Aboveground biomass would be as the best indirect selection traits on breeding high yield of P. heterophylla, the first branch number, width of leaves and cleistogamous flowers world be as a better auxiliary index on breeding high variety of P. heterophylla.</p>

SRAP analysis on genetic diversity for germplasm resources of Dipsacus asper / 中草药

Objective: To study the genetic diversity of Dipsacus asper from different populations and provide a reference for the rational utilization of its germplasm. Methods: The genetic diversity of the 14 populations of D. asper was analyzed by SRAP molecular markers. Results: Ten pairs of primers produced 124 sites, among which 102 were polymorphic sites. The percentage of polymorphic loci (PPL) was 82.26%. The Nei's genetic diversity index (H) and the Shannon's information index (I) were 0.2800 and 0.4353, respectively. At the population level, PPL was 53.92%, H was 0.1212-0.2440, and I was 0.1796-0.3611. The genetic diversity values of the five populations were relatively high, and the populations had the characteristics of high altitude and microhabitat. Genetic differentiation coefficient (Gst) was 0.2930, gene flow (Nm) was 1.2064. Cluster analysis based on genetic similarity indicated that the 14 populations could be divided into three groups. Conclusion: The genetic diversity among the populations of D. asper was at relatively high level. The genetic variance of D. asper mainly existed within the populations. The high genetic diversity could be attributed to the geographical position (altitude) and climate, while geographic isolation (microhabitat) was another important factor for the genetic variance within the populations.

Variation of icariin and total flavonoid of Epimedium acuminatum in different parts and habitats / 中国中药杂志

<p><b>OBJECTIVE</b>Through comparative study on contents of icariin and total flavonoid of Epimedium acuminatum in different habitats and parts, the distribution and correlation of the two components were observed.</p><p><b>METHOD</b>Twenty-four sample spots in four habitats were set up, and the whole plant of these samples was divided as following: leaf, root, stem and rhizome. Total flavonoid and icariin of samples were determined by using the UV method and the HPLC, respectively. The data was analyzed by SPSS 17.0.</p><p><b>RESULT</b>The distribution of icariin in different parts had the pattern: leaf > root > stem > rhizome, the total flavonoid content was higher in leaf, but it showed no difference in other parts. Habitat had certain effect on icariin accumulation in E. acuminatum. The significant differences of total flavonoid content in E. acuminatum from different parts were not observed.</p><p><b>CONCLUSION</b>The distribution of icariin from different parts and habitats has high selectivity. Metabolism and accumulation of flavonoid content in each part have no difference. Flavonoid content is less affected by environment. Considering the distribution of icariin and flavonoid content in every part and the growth strategy in different habitats comprehensively, it is reasonable to harvest the aerial part for the sustainable utilization of E. acuminatum.</p>

Seed dormancy mechanisms of Pseudostellaria heterophylla and its germination characteristics / 中国中药杂志

<p><b>OBJECTIVE</b>Seed dormancy mechanisms, breaking dormancy and the optimal germinating conditions were studied to improve reproductive efficiency and quality standard of Pseudostellaria heterophylla.</p><p><b>METHOD</b>The P. heterophylla seeds were stratified into wettish sand for 65 days under the temperature of -2-3 degrees C, embryo length and germination dynamic change of seeds were measured in prophase every other 10 days and in middle every other 5 days. The influences on seed germination and seedling growth under different temperatures, germination beds were investigated.</p><p><b>RESULT</b>The P. heterophylla seeds embryo morphology was unchanged in stratification. The seeds could not germinate at stratification within the first 35 days. The germination rate and germination energy of seeds increased with the stratification time after 35 days. Germination rate, germination energy, plant height and fresh weight under the 10 degrees C were significantly higher than those under other temperatures. Germination rate, germination energy, plant height, fresh weight and dry weight of plant in sand bed were higher than those of plant in other beds, and seed mildew rate and abnormal seedling proportion of plant in sand bed were less than those of plant in other beds.</p><p><b>CONCLUSION</b>The dormancy of P. heterophylla seeds is mainly the physiological post-maturation. The optimal germination conditions of seeds is 10 degrees C the culture of sand. The dormancy was broke under -2-3 degrees C of wettish sand for 45-50 days in practice.</p>

Variation and regularity of volatile oil constituents in fruits of national medicine Cinnamomum migao / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the constituents of the volatile oil in Cinnamomum migao from different regions of southwest in China in order to evaluate the quality of C. migao.</p><p><b>METHOD</b>GC-MS was employed to analyze the constituents of the volatile oil in C. migao.</p><p><b>RESULT</b>The volatile oil compositions of C. migao collected from 27 of cultivation regions were obviously different. Based on the chemical differences of the volatile oil compositions, C. migao was divided into four chemotype, they were eucalyptol, eucalyptol -cyclohexene, eucalyptol -alpha-terpineol, and eucalyptol -sabinene. The eucalyptol-type was cultivated in Luodian, guizhou province and Funing regions, Yunan province. The eucalyptol-cyclohexene-type was cultivated in Zhengfeng and Wangmo regions, Guizhou province. The type of eucalyptol, eucalyptol -sabinene and eucalyptol -alpha-terpineol were cultivated in Ceheng and Libo regions, Guizhou province.</p><p><b>CONCLUSION</b>Combined with the geographical distribution, It is indicated that the volatile oil compositions in fruit of C. migao may have some relations to the specie itself characteristics and different elevations environment.</p>