A Bi-Directional Acoustic Micropump Driven by Oscillating Sharp-Edge Structures.

This paper proposes a bi-directional acoustic micropump driven by two groups of oscillating sharp-edge structures: one group of sharp-edge structures with inclined angles of 60° and a width of 40 µm, and another group with inclined angles of 45° and a width of 25 µm. One of the groups of sharp-edge structures will vibrate under the excitation of the acoustic wave generated with a piezoelectric transducer at its corresponding resonant frequency. When one group of sharp-edge structures vibrates, the microfluid flows from left to right. When the other group of sharp-edge structures vibrates, the microfluid flows in the opposite direction. Some gaps are designed between the sharp-edge structures and the upper surface and the bottom surface of the microchannels, which can reduce the damping between the sharp-edge structures and the microchannels. Actuated with an acoustic wave of a different frequency, the microfluid in the microchannel can be driven bidirectionally by the inclined sharp-edge structures. The experiments show that the acoustic micropump, driven by oscillating sharp-edge structures, can produce a stable flow rate of up to 125 µm/s from left to right, when the transducer was activated at 20.0 kHz. When the transducer was activated at 12.8 kHz, the acoustic micropump can produce a stable flow rate of up to 85 µm/s from right to left. This bi-directional acoustic micropump, driven by oscillating sharp-edge structures, is easy to operate and shows great potential in various applications.

Identification of LCA-binding Glycans as a Novel Biomarker for Esophageal Cancer Metastasis using a Lectin Array-based Strategy.

Esophageal cancer (EC) is a unique and heterogeneous disease diagnosed mostly at advanced stages. Altered glycans presented on cell surfaces are involved in the occurrence and development of malignancy. However, the effects of glycans on EC progression are largely unexplored. Here, a lectin array was utilized to detect the glycan profiling of the normal esophageal mucosal epithelial cell line and two EC cell lines. The binding of Lens culinaris lectin (LCA) to EC cells was found to be stronger than that of the normal cells. Lectin immunohistochemical staining revealed that LCA-binding glycans were markedly elevated in EC tissues compared to adjacent non-cancerous tissues. LCA staining was significantly associated with lymph node metastasis, depth of invasion, TNM stage and poor overall survival of EC patients. Added LCA to block LCA recognized glycans could inhibit the migration and invasion of EC cells. Further analysis revealed that blocking the biosynthesis of LCA-binding glycans by tunicamycin attenuated cellular migratory and invasive abilities. Additionally, a membrane glycoprotein CD147 was recognized as a binder of LCA. There was a positive correlation between LCA-binding glycans and CD147 expression in clinical samples. Interestingly, CD147 inhibition also reduced cell migration and invasion. These findings indicated that LCA-binding glycans may function as a novel indicator to predict metastasis for patients with EC.