Application and recent progress of inland water monitoring using remote sensing techniques.

Hyperspectral remote sensing, which retrieves the water quality parameters by direct high-resolution analysis of the electromagnetic spectrum reflected from the water surface, has been widely applied for inland water quality detection. Such a new approach provides an opportunity to generate real-time data from water with the noncontact method, largely improving working efficiency. By summarizing the development and current applications of hyperspectral remote sensing, we compare the relative merits of varying remote sensing platforms, popular inversion models, and the application of hyperspectral monitoring of chlorophyll-a (Chl-a), transparency, total suspended solids (TSS), colored dissolved organic matter (CDOM), phycocyanin (PC), total phosphorus (TP), and total nitrogen (TN) water quality parameters. Most studies have focused on spaceborne remote sensing, which is usually used to monitor large waterbodies for Chl-a and other water quality parameters with optical properties; semiempirical, bio-optical, and semianalytical models are frequently used. With the rapid development of aerospace technology and near-surface remote sensing, the spectral resolution of remote sensing imaging technology has been dramatically improved and has begun to be applied to small waterbodies. In the future, the multiplatform linkage monitoring approach may become a new research direction. Advanced computer technology has also enabled machine learning models to be applied to water quality parameter inversion, and machine learning models have higher robustness than the three commonly used models mentioned above. Although nitrogen and phosphorus, with nonoptical properties, have also received attention and research from some scholars in recent years, the uncertainty of their mechanisms makes it necessary to maintain a cautious attitude when treating such research.

Genome Evolution of Filamentous Cyanobacterium Nostoc Species: From Facultative Symbiosis to Free Living.

In contrast to obligate bacteria, facultative symbiotic bacteria are mainly characterized by genome enlargement. However, the underlying relationship of this feature with adaptations to various habitats remains unclear. In this study, we used the global genome data of Nostoc strains, including 10 novel genomes sequenced in this study and 26 genomes available from public databases, and analyzed their evolutionary history. The evolutionary boundary of the real clade of Nostoc species was identified and was found to be consistent with the results of polyphasic taxonomy. The initial ancestral species of Nostoc was demonstrated to be consistent with a facultative symbiotic population. Further analyses revealed that Nostoc strains tended to shift from facultative symbiosis to a free-living one, along with an increase in genome sizes during the dispersal of each exterior branch. Intracellular symbiosis was proved to be essentially related to Nostoc evolution, and the adaptation of its members to free-living environments was coupled with a large preference for gene acquisition involved in gene repair and recombination. These findings provided unique evidence of genomic mechanisms by which homologous microbes adapt to distinct life manners and external environments.

Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing.

Microcystis is one of the most common and dominant bloom-forming cyanobacteria in freshwater worldwide. The method for genotype detection based on traditional molecular cloning is expensive and time consuming and generates a limited number of sequences. In this study, a high-throughput sequencing (HTS) method was developed to detect the internal transcribed spacer (ITS) regions between 16S and 23S rRNA region of Microcystis populations along a typical water system in Yuqiao Reservoir-Haihe River in Tianjin, northern China. A total of 629,341 reads were obtained and clustered into 2005 operational taxonomic units (OTUs). Analysis of alpha diversity indices showed that the Haihe River is more diverse than Yuqiao Reservoir. In general, the two water areas exhibit a clear differentiation pattern in OTU abundance, sharing genotypes from a small part of Yuqiao Reservoir with those in the Haihe River. Phylogenetic analysis further indicated the possible flexible evolution of Microcystis genotypes occurring in the research areas. This study provides the first exhaustive description of HTS method for detection of ITS region to evaluate Microcystis intra-species diversity and relationship.