Biological characteristics and construction of stable ALDH-1 knock-down Hela cell lines.

OBJECTIVES: To explore the relationship between aldehye dehydrogenase-1 (ALDH-1) and biological characteristics of the stable ALDH-1 knock-down Hela cell lines. MATERIALS AND METHODS: Transfected Hela cells with lentiviral vector were utilized and puromycin was used to screen and achieve the stable cell lines. The interference efficiency was calculated by performing qRT-PCR to detect the expression of ALDH-1 of three groups including the interfering group, the negative control (NC) group, and the normal Hela group. CCK-8 assays were used to detect the OD value of each group. The coloning formation rate of each group was detected by colony formation assay. cell cycle distribution and apoptosis of each group were also detected by employing cell cycle and apoptosis experiments. Results: The stable ALDH-1 knock-down Hela cell lines were successfully obtained after two weeks' screening; compared with the NC and Hela group, the ALDH-1 expression level of interfering group was 1.05 ± 0.10 (both p < 0.05), whose silencing efficiency was 80.59%. CCK-8 assays verified that the mean OD value of interfering group was lower than that of NC and Hela group. Additionally, colony formation assays showed that the coloning efficiency of interfering group was lower than that of NC and Hela group. Cell cycle experiments proved that the proportion of G0/G1 phase of interfering group was higher than that of the other two groups, while the proportion of S phase was lower. Cell apoptosis assays indicated that the apoptosis rate of interfering group was the highest. CONCLUSIONS: Constructing stable interfering Hela cell lines with lentiviral vectors was successful and worthy of promotion. ALDH-1 plays an important role in promoting cell growth and proliferation, maintaining cell cycle and inhibiting Hela cell apoptosis.

APOBEC3A possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer.

Cervical cancer is the second most common cancer among women worldwide and is the leading cause of deaths in developing countries. Persistent infections with a subset of HPVs, called "high-risk HPVs", including HPV16 and HPV18, are the primary cause of cervical cancer. The apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) family of proteins is a group of cellular enzymes that catalyze the deamination of cytidine (C) to uracil (U) in single-stranded DNA/RNA, and functions as antiviral factors in the innate immune system of the host. Recent studies have shown that APOBEC3A could restrict certain DNA viruses, including HPVs. In this study, we confirmed that the expression of APOBEC3A was decreased in cervical cancer tissues. Furthermore, APOBEC3A inhibited the cervical cells proliferation, migration as well as invasion, and promoted apoptosis depend on cytidine deaminase. In addition, APOBEC3A decreased HPV16-E6, HPV16-E7 and HPV18-E6 depend on cytidine deaminase, but no effect on HPV18-E7. Therefore, we believe that, in cervical cancer cells, the expression of APOBEC3A possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression depend on cytidine deaminase.

[Evaluation of the effect of post-abortion counseling and education among unmarried abortion adolescents].

OBJECTIVE: To find out the requirement and to evaluate the effect of post-abortion counseling and education (PACE) among unmarried abortion adolescents. METHODS: The subjects of the study were unmarried adolescents from 10 to 24 years of age who wanted induced abortion in the Second Affiliated Hospital of Sun Yat-sen University from December 2007 to April 2008. Totally 122 subjects received the intervention of PACE were considered as intervention group. Meanwhile, 67 subjects refused the intervention of PACE were considered as no intervention group. Two groups were both investigated the requirements of PACE before abortion and were followed-up at one year after abortion. RESULTS: 97.4% (184/189) of 189 unmarried abortion adolescents were willing to receive PACE, 48.1% (91/189) of them hoped to receive PACE when saw the doctor, 72.0% (136/189) of them required face-to-face counseling during PACE. During the year after abortion, 74% (57/77) cases in intervention group and 24% (10/41) cases in no intervention group took effective contraception (P < 0.01), while 1% (1/77) of intervention group and 10% (4/41) of no intervention group had unwanted pregnancy. There were significant different for the rate of unwanted pregnancy between two groups (P = 0.034). CONCLUSION: For unmarried abortion adolescents, the intervention of PACE may markedly increase the rate of effective contraception used and decrease the rate of another unwanted pregnancy.