RESUMO
Avian encephalomyelitis virus (AEV) can be transmitted both horizontally and vertically. In the present study, we report a typical case of AEV infection in broiler breeder chickens and their progeny identified by clinical survey of the disease, antibody detection, and reverse transcription (RT)-polymerase chain reaction (PCR) assay.
Assuntos
Anticorpos Antivirais/sangue , Galinhas/virologia , Vírus da Encefalomielite Aviária/imunologia , Transmissão Vertical de Doenças Infecciosas , Infecções por Picornaviridae/veterinária , Doenças das Aves Domésticas/transmissão , Animais , Anticorpos Antivirais/biossíntese , Antígenos Virais/imunologia , Encéfalo/virologia , Proteínas do Capsídeo/imunologia , Galinhas/imunologia , China/epidemiologia , Surtos de Doenças , Vírus da Encefalomielite Aviária/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Feminino , Infecções por Picornaviridae/sangue , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/transmissão , Doenças das Aves Domésticas/sangue , Doenças das Aves Domésticas/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
The open reading frame of peacock and parakeet prion protein (PrP) genes was cloned and sequenced. The peacock and parakeet PrP genes consisted of 833 and 866 nucleotides encoding 266 and 277 amino acids, respectively (GenBank Accession numbers AY365065 and AY365066). Sequence analysis showed that the peacock and parakeet PrP genes had 93.67% homology to each other, 94.04% and 99.64% homology to the chicken PrP gene and 46.0% and 42.1% similarity to the mammalian PrP genes, respectively. The structural features of all known mammalian and avian PrPs, including N-terminal signal peptides, tandem repeats, conserved hydrophobic region, disulfide bridges and glycoinositol phospholipid anchor, were also found in peacock and parakeet PrPs. The parakeet and peacock PrPs, however, differed in the hexarepeat region, with the peacock having six and the parakeet having seven hexarepeats. The phylogenetic analysis showed that the PrP genes in 52 species were clustered into 2 distinct lineages, the avian and the mammalian. The peacock and parakeet PrP genes belonged to the same lineage but the peacock PrP was sub-classed with the pigeon PrP and the parakeet PrP was sub-classed with the duck and chicken PrPs. The present work added two more species data to the collection of the PrP genes and supported the previous findings that the PrP genes are highly conserved across species.
