RESUMO
One new lignan (7S,8R,7'R,8'R)-7-(3,4-methylenedioxyphenyl)-8,8'-dimethyl-8'-hydroxyl-7'-methoxyl-7'-(3',4'-methylenedioxyphenyl)-tetrahydrofuran (1), one new sesquiterpene 2-hydroxy-11,12-dehydrocalamenene (2), one new natural product erythro-1-(3,4-dimethoxyphenyl)-4-(3,4-methylenedioxyphenyl)-2,3-dimethyl-butane (3), and two known lignans (+)-anwulignan(erythro-1-(4-hydroxy-3-methoxyphenyl)-4-(3,4-methylenedioxyphenyl)-2,3-dimethyl-butane) (4) and ( - )-zuonin-A (5) were isolated from the stems of Schisandra glaucescens Diels. Their structures were elucidated by spectroscopic methods. The cytotoxicity of compounds 1 and 2 was assayed.
Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Lignanas/isolamento & purificação , Schisandra/química , Sesquiterpenos/isolamento & purificação , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Furanos , Células HCT116 , Humanos , Lignanas/química , Lignanas/farmacologia , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Caules de Planta/química , Sesquiterpenos/química , Sesquiterpenos/farmacologia , EstereoisomerismoRESUMO
protein was found in E.coli which can specifically bind to NF-IL6 mRNA 3'UTR. After a series of purification steps, the RNA-specific binding protein was directly sequenced on the Porton LF3200 Protein Sequencer. A sequence of 10 amino acids of the purified NF-IL6 3'UTR binding protein was obtained, namely, Ala-Thr-Arg-Ile-Glu-Phe-His-Gly-Cyss(?)-Gly. A BLAST search with the 10 amino acids against NCBI database failed to identify any protein with identical sequence. The significance of identifying an eukaryotic mRNA binding protein in E.coli is discussed.
