Nanoparticles-EPS corona increases the accumulation of heavy metals and biotoxicity of nanoparticles.

Microbial extracellular polymeric substances (EPS) coating nanoparticles (NPs) surface can form NPs-EPS corona, which significantly affect the adsorption of NPs to toxic substances and alter the ecotoxicological effect of NPs. In this work, the EPS coronas on TiO2 NPs (TNPs) and CeO2 NPs (CNPs) were characterized and the adsorption characteristics of NPs with and without EPS corona to five heavy metals were investigated in single-metal and multiple-metal systems. The results of spectral analysis showed that NPs-EPS corona exhibited new crystalline phases and abundant functional groups. Moreover, 42 and 13 proteins were identified in the TNPs-EPS and CNPs-EPS coronas, respectively. The rates of Cd2+, Pb2+, Cu2+, Ni2+ and Ag+ adsorption by NPs-EPS corona increased to values that were 6.7-7.6, 4.4-5.1, 4.2-5.5, 3.9-4.9 and 8.5-8.8 times those of NPs without EPS corona, respectively, in single-metal system. NPs-EPS coronas are effective in absorbing Ag+, Pb2+ and Cu2+ compared with Cd2+and Ni2+ in multiple metal adsorption. These results indicated that NPs-EPS corona effectively adsorb and remove heavy metals by forming NPs-EPS-metal complexes and inducing precipitation. However, NPs-EPS corona can enhance the toxicity of NPs by accumulating highly-toxic heavy metals in aquatic environments.

Identification and function of extracellular protein in wastewater treatment using proteomic approaches: A minireview.

Microbial extracellular proteins serve as important functions in wastewater treatment process. Analysis of their compositions and properties is crucial to probe their specific functions. However, conventional analytical techniques cannot obtain interest protein information from complex proteins. Recently, the extracellular proteomics method has been applied to resolve the composition of extracellular proteins. In order to better understand the roles of extracellular protein in wastewater treatment process, this review provides the information on the proteomics methods and their application in investigating extracellular proteins involved in microbial attachment/aggregation, biodegradation of pollutants, and response to environmental stresses. Future work needs to exploit the full capability of the proteome.