RESUMO
Cell surface engineering is an emerging technology to encapsulate cells in order to enhance their functions. However, methods for reversible encapsulation of cells with abiotic functionalities are rare. Herein, we describe a phenylboronic acid based click reaction for encapsulation of single yeast cells using mesoporous silica nanoparticles (MSNs). This encapsulation does not impact natural growth of the cells and leads to a significant enhancement of cell survival in a variety of hostile environments. Owing to the glucose-responsiveness of the boronate ester bond between cell surface polysaccharides and B(OH)2-grafted MSNs, encapsulation was reversible by addition or removal of glucose. This effort offers living cells effective protection under harsh conditions and enables reversible assembling-detaching of abiotic functions.
Assuntos
Células Imobilizadas/citologia , Química Click/métodos , Saccharomyces cerevisiae/citologia , Ácidos Borônicos/química , Viabilidade Microbiana , Nanopartículas/química , Nanopartículas/ultraestrutura , Porosidade , Saccharomyces cerevisiae/ultraestrutura , Dióxido de Silício/químicaRESUMO
This paper reviews the research progress in telemonitoring systems and automatic analysis of ECG signals. The key problems, urgently to be solved, about the automatic analysis in ECG telemonitoring systems are discussed.
Assuntos
Algoritmos , Redes de Comunicação de Computadores , Eletrocardiografia Ambulatorial , Desenho de Equipamento , Processamento de Sinais Assistido por Computador , Telecomunicações , TelemetriaRESUMO
Wearable monitoring technology is a hot spot in biomedical engineering and military medical devices. This paper reviews the research progress about the wearable life-sign monitoring system for a single soldier.