Characteristics and abortion cytological pathways of male sterility of Bupleurum chinense / 中国中药杂志

<p><b>OBJECTIVE</b>To study the characteristics of male sterility of Bupleurum chinense and further explore the developmental period and reason of abortion for the male sterile plants.</p><p><b>METHOD</b>The morphological characteristics of B. chinense male sterile and normal plants were investigated and compared. The anther development process and pollen viability of two types of plants were examined by microscopic assay.</p><p><b>RESULT AND CONCLUSION</b>The shapes and sizes of anther and filament were different between the male sterile and the normal plants. For the male sterile plant's, the filament size was no more than 1/2 of that of normal plants and the anthers were shriveled, failed to dehisce and pollinate naturally, and the pollen grains in the anthers had no vitality. Other morphological characteristics were similar between two types of plants. The main reason leading to male sterility of B. chinense was the abnormal development of tapetum cells with two circumstances. The one is that the tapetum cells degraded early during the period of microsporocyte phase to tetrad phase and the other is that the tapetum cells proliferated with delayed degradation in the tetrad to uninucleate phase.</p>

Breeding of new varieties "zhongchai no. 2" and "zhongchai no. 3" of Bupleurum chinense / 中国中药杂志

<p><b>OBJECTIVE</b>To breed new varieties with better uniformity and commercial quality as well as higher saikosaponin contents.</p><p><b>METHOD</b>The excellent germplasm resources were selected from "zhongchai no. 1" population. Single plant method was applied to get better varieties. All the breeding material was investigated according to morphological characters, agronomic characters and the contents of saikosaponin a and saikosaponin d. The experiments of comparative test and varieties regional test were carried out.</p><p><b>RESULT</b>The bred new varieties of "zhongchai No. 2" and "zhongchai No. 3" had better uniformity. The dark brown roots ratios of the two varieties were 83.2%, 89.9%, respectively. The contents of saikosaponins (a + d) of the two varieties reached 1.31%, 1.02%, respectively.</p><p><b>CONCLUSION</b>"zhongchai No. 2" and " zhongchai No. 3" both had the advantages of better uniformity, darker brown roots and higher saikosaponin contents.</p>

Comparison on agronomy and quality characters of selective strain of Schizonepeta tenuifolia / 中国中药杂志

<p><b>OBJECTIVE</b>With the purpose of selecting adequate quality and high production of Schizonepeta tenuifolia, the comparative experiments were carried out on different strain of S. tenuifolia in 2007.</p><p><b>METHOD</b>The test fields were divided into blocks randomly, and the agronomy characters were investigated in harvest time; the content of volatile oil was measured by steam distillation and the pulegone were determined by HPLC.</p><p><b>RESULT</b>The yield of S4 was 18.63% and 29.99% higher than that of CK1 and CK2, respectively. The contents of volatile oil and pulegone were also higher than those of CK and other strains in this test.</p><p><b>CONCLUSION</b>S4 shows the advantages of high production, strong disease resistance and high active components. S4 would be extended as the good breed in production.</p>