Survivin antisense oligonucleotide induces apoptosis of SGC7901 cells and enhances sensitivity to taxol / 临床与实验病理学杂志

Purpose To investigate the anti-tumor effect of survivin antisense oligonucleotide (Asodn),and whether it can strengthen the sensitivity of chemical therapy with taxol.Methods The experiment was devided into four groups:control group,liposome group,sense oligonucleotide group,and antisense oligonucleotide group.Survivin antisense oligonucleotide was synthesized,and transfected into gastric cancer cells with liposome.The inhibitory rate of proliferation was tested with MTT,and the expression of survivin protein with Western blot;the morphological changes of apoptosis through Hoechst staining were observed, and the apoptosis rate with flow cytometer.Results As observed through Hoechst staining, the cancer cells had normal blue nucleus in the control group, Lip group, and Sodn group, while in Asodn transfection group the nucleus became condensed, with karyorrhexis. The cell inhibitoty rate in Asodn group increased, presenting in a time-dosage dependence manner; survivin protein expression reduced and apoptotic rate increased; there were differences in statistical significance (P<0.05), as compared with the control group, Lip group and Sodn group. The proliferation-inhibitory rate in Asodn plus taxol group [(78.1±0.8) %] was obviously higher than that in Asodn group [(54.9±1.6)%] and taxol group [(56.7%±0.7)%] (P<0.05).Conclusion Survivin antisense oligonucleotide can inhibit the proliferation of gastric cancer cells, induce apoptosis, and strengthen the effect of taxol on the inhibition of tumor growth.

Strengthening and improving the ideological and political work of students in independent colleges / 中华医学教育探索杂志

Independent Institute is a model of exploration and innovation under a new situation of higher education mechanism and model and its students ideological and political work has its own particularity.This paper analyses the particularity of the ideological and political work of independent institute students,and discusses how to strengthen and improve the ideological and political work of independent institute students.

Invasive ductal carcinoma of the breast is prevalence of loss of heterozygosity at 8p22,11p15 and 17p13 / 医学研究生学报

Objective:To investigate the LOH at regions on chromosomal arm 8p22,11p15,17p13 and its relationship with clinicopathological parameters. Methods:Thirty-four paraffin-embedded tumor and corresponding noncancerous tissues were analysed. PCR was used to amplified three microsatellite markers D8S136,D11S988 and TP53 located at these chromosomal regions. PCR products were electrophoresed on 6%polyacrylamide gel and detected using silver staining. The P53, c-erBb-2,PR,ER status were determined by immunohistochemistry. Results:Of the three markers we studied, D8S136 was detected LOH at a frequency of 12 in 34 tumors(35.29%). D11S988 and TP53 were detected LOH at a frequency of 5 in 34 tumors(14.71%). There were no obvious associations between LOH at D11S988、TP53 and clinicopathological parameters, but the tumors with LOH at D8S136 were significant larger than that without LOH(P=0.0049). Conclusion: Invasive ductal carcinoma of the breast has a frequent LOH on chromosome 8p22. The loss or inactivation of putative tumor suppressor genes on 8p22 may contribute to the excessive growth of the tumors.

Roles of cyclin D1 and cyclin E in non-small-cell lung cancer and the relationship between cyclin D1 and cyclin E / 中国癌症杂志

Purpose:To study the roles of cyclin D1 and cyclin E in non small cell lung cancer(NSCLC) and the relationship between cyclin D1 and cyclin E.Methods:The expression of cyclin D1 and cyclin E in 87 NSCLC were studied immunohistochemically, PCNA was detected immunohistochemically to estimate the proliferative index(PI), the relationship between cyclin D1, cyclin E, PI, clinicopathological and prognostic factors were analysed.Results:Of the 87 NSCLC, 44 8%(39/87) were positive for cyclin D1 and 48.3%(42/87) for cyclin E, the PI of the cyclin D1 positive group was significantly higher than the cyclin D1 negative group( P 〈0.05).Significant differences were shown in the diameter of tumor, lymph node metastasis rate and survival rate between the positive and negative group of cyclin D1( P 〈0.01,0.05,0.01), and there were significant differences in lymph node metastasis, stages and survival rate between the positive and negative group of cyclin E( P 〈0.05,0.05,0.01). The expression of cyclin E in the cyclin D1 positive group was significantly higher than that of the cyclin D1 negative group( P 〈0.05), and the PI, diameter of tumor, lymph node metastasis rate in the coexpression group of cyclin D1 and cyclin E were significantly higher than that of the non coexpression group( P 〈0.05), while the coexpression group had significantly lower survival rate than that of the non coexpression group( P 〈0.05).Conclusions:Cyclin D1 and cyclin E are involved in NSCLC, but their effects are different: cyclin D1 is an important factor in the regulation of proliferation, cyclin E is related to the progression of NSCLC, cyclin D1 can promote the expression of cyclin E, while cyclin E can perhaps intensify the effect of cyclin D1, thus there could exist synergism between cyclin D1 and cyclin E.

Expression of cyclin D1 and E in lung cancer / 第二军医大学学报

Objective: To study the expression of cyclin D1 and E in lung cancer. Methods: The immunohistochemical technique was used to detect the expression of cyclin D1 and E in 68 cases of non small cell lung cancer(NSCLC) and 21 cases of small cell lung cancer(SCLC). Results: The rate of cyclin D1 protein positive expression was 48.5% in NSCLC, 4.8% in SCLC,showing significant difference( P