[Risks to predict blood loss and cranial nerve injury in carotid body paraganglioma resection].

Objective: To investigate clinical and imaging parameters to predict blood loss and cranial nerve injury (CNI) following carotid body paraganglioma (CBP) resection. Methods: A retrospective examination of clinical and imaging data was conducted on 63 patients who underwent CBP resection at Xiangya Hospital of Central South University from January 2016 to December 2022, including 23 males and 40 females, aged 26-87 years old. Three imaging parameters including tumor volume, the angle of contact with the internal carotid artery (ICA), and the distance to the base of skull (DTBOS) were gauged using the IMEDPACS software on CTA and MR imaging. The predictive efficacies of age, gender, Shamblin classification, and three imaging parameters for blood loss and CNI following surgery were analysed. Logistic composite parameter models were constructed and their predictive validity was assessed. Results: Multivariate logistic regression analysis underscored that only tumor volume (OR=1.381,95%CI:1.167-1.507,P=0.001) showed significant statistical correlations with blood loss following surgery. Area under curve (AUC) values of 0.910 for receiver operating characteristic (ROC) curves showed a sensitivity of 1.000 and a specificity of 0.694. Tumor volume (OR=1.126,95%CI:1.030-1.231, P=0.002) and DTBOS (OR=0.225,95%CI:0.081-0.630,P=0.005) were significantly associated with postoperative CNI. The analysis of logistic composite model showed AUC values for tumor volume, DTBOS and combination of the two parameters were 0.858, 0.788, and 0.872, respectively. The model for combination of tumor volume and DTBOS also proved superior in predicting postoperative CNI (Z=3.106, P<0.001), with a sensitivity of 0.833 and a specificity of 0.769. Conclusions: Tumor volume and DTBOS emerged as effective predictors for blood loss and/or CNI in patients with CBP resection. Moreover, the logistic composite parameter model outclassed single-parameter models in terms of their predictive clinical value.

Surface modification using photocrosslinkable chitosan for improving hemocompatibility.

Immobilization of the anticoagulative or antithrombogenic biomolecule has been considered as one of the important methods to improve the blood compatibility of artificial biomaterials. In this study, a novel immobilization reaction scheme was utilized to incorporate O-butyrylchitosan (OBCS) onto the activated glass surface with an aim to develop an anticoagulative substrate. Activation of the glass surface was carried out by silanization and then OBCS was grafted to the silanized surface via a radiation grafting technique. The OBCS-grafted glass surfaces were characterized by electron spectroscopy for chemical analysis (ESCA) and atomic force microscopy (AFM). The blood compatibility of the OBCS-grafted glass was evaluated by platelet rich plasma (PRP) contacting experiments and protein adsorption experiments in vitro. These results have demonstrated that the surface with immobilized OBCS shows much less platelet adhesive and fibrinogen adsorption compared to the control surface. Therefore, the novel reaction scheme proposed here is very promising for future development of an anticoagulative glass substrate.

[A nasopharyngeal carcinoma negatively related EST on 7q32].

To isolate and clone the tumor suppressor gene on chromosomal region 7q32 that corelated with the occurrence of human NPC, we detected the genotype of polymorphic microsatellite markers on 7q32 in 24 nasopharyngeal carcinoma biopsies and matched normal lymphocyte DNA. LOH was found in 30% biopsies. Using differential RT-PCR and Northern hybridization we compared the expression level of 20 EST on 7q32 between NPC cell line HNE1 and primary culture of normal nasopharyngeal epithelial cell, and found AA070437 EST expressed high in primary culture of normal nasopharyngeal epithelial cell, but very low in HNE1. Differential RT-PCR (dRT-PCR) analysis showed that the expression level of AA070437 was lower in 30.7% NPC biopses than in normal cell. Differential PCR (dPCR) showed that allelic loss of AA070437 was observed in 29.1% NPC biopses. This EST is a part of sequence of a new gene compared with GeneBank database. Our results showed that AA070437 EST negatively related with the occurrence of human NPC is a candidate of tumor suppressor gene of NPC on 7q32.