RESUMO
The aim of the present study was to investigate the antioxidant response mechanism of epigallocatechin3gallate (EGCG) in H2O2induced mouse renal tubular epithelial cells (MRTECs). The cultured MRTECs were divided into normal, H2O2 (control) and EGCG treatment groups. The MTT assay was used to assess cell viability, and reverse transcriptionquantitative polymerase chain reaction (RTqPCR), immunocytochemical and western blot analyses were performed to detect the expression of nuclear factor erythroid 2related factor 2 (Nrf2) and γglutamyl cysteine synthetase (γGCS). EGCG was able to mitigate H2O2mediated cell damage. The RTqPCR results demonstrated that EGCG was able to upregulate the gene expression of Nrf2 and γGCS in MRTECs in a dosedependent manner. The immunocytochemistry and western blot analyses demonstrated that EGCG was able to increase the protein expression of Nrf2 and γGCS in MRTECs in a dosedependent manner. Oxidative stress may lead to a decrease in the viability of MRTECs, while EGCG was able to promote the expression of Nrf2 and γGCS in MRTECs, thereby improving the antioxidant capacity of the cells and promoting the repair of oxidative stress injury.