RESUMO
The method of high-performance liquid chromatography (HPLC) with UV-vis detection was used and validated for the simultaneous determination of six flavonoids (puerarin, rutin, morin, luteolin, quercetin, kaempferol) and troxerutin in rat urine and chicken plasma. Chromatographic separation was performed using a VP-ODS column (150 mm x 4.6 mm, 5.0 microm) maintained at 35.0 degrees C. The mobile phase was a mixture of water, methanol and acetic acid (57:43:1, v/v/v, pH 3.0) at the flow rate of 0.8 mL/min. Six flavonoids and troxerutin were analyzed simultaneously with good separation. On optimum conditions, calibration curves were found to be linear with the ranges of 0.10-70.00 microg/mL (puerarin, rutin, morin, luteolin, quercetin, kaempferol) and 0.50-350.00 microg/mL (troxerutin). The detection limits were 0.010-0.050 microg/mL. The method was validated for accuracy and precision, and it was successfully applied to determine drug concentrations in rat urine and chicken plasma samples from rat and chicken that had been orally administered with six flavonoids and troxerutin.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/isolamento & purificação , Hidroxietilrutosídeo/análogos & derivados , Espectrofotometria Ultravioleta/métodos , Animais , Galinhas , Flavonoides/sangue , Flavonoides/urina , Hidroxietilrutosídeo/sangue , Hidroxietilrutosídeo/isolamento & purificação , Hidroxietilrutosídeo/urina , Ratos , Sensibilidade e EspecificidadeRESUMO
It was found that in buffer solution of pH 7.0, the addition of sodium dodecyl sulfate (SDS) to the solution of phenothiazine drugs, such as chlorpromazine, promethazine and trifluoperazine, showed a remarkable enhancement of their fluorescence intensity. A further study proved that the phenothiazine drugs can be determined by fluorophotometric method in micellar system. Under optimal conditions, there was a good linear relationship between fluorescence intensity and phenothiazine compounds concentration, and the detection limit of 3.0 x 10(-8) M chlorpromazine, 3.0 x 10(-8) M promethazine and 1.5 x 10(-8) M trifluoperazine (S/N=3) were also obtained. This method has been used to determine phenothiazine drugs in tablets with satisfactory results.
