THE IMMUNOHISTOCHEMICAL STUDY OF HUMAN OVARIN TISSUE USING MONOCLONAL ANTIBODIES AGAINST PORCINE ZONA PELLUCIDA(ZP-OVA) / 解剖学报

Three hybridomas producing monoclonal antibodies (Mab) to zona pellucida of porcine ovarian egg(ZP-OVA) were established(LPDg, LPC4, LPD,).Two Mab(LPD_8 LPC_4) were examined against human ovarian tissue by immunohistochemical technique in vitro. We found that the two Mab had cross-reaction with zona pellucida and ovum cytoplasm and no cross-reaction with membrana granulosa and theca folliculi. It showed that there were affinities of LPD_8 and LPC_4 to antigenic determinamts in the ovum cytoplasm and common antigenic components in zona pellucida and ovum cytoplasm. It suggested that the zona pellucida was derived from oocyte.

ROSETTE INHIBITION OF ANTIHUMAN LYMPHOCYTE GLOBULIN(ALG)ON HUMAN PERIPHERAL LYMPHOCYTES IN VITRO / 解剖学报

The inhibition of lymphocyte rosette formation by ALG was performed in vitro.The rosette inhibition titer was 1:1,600.It was considered that complement playedno important role in the inhibiting effect of ALG on the rosette formation but incytotoxin.Since the rosette inhibition titer of ALG was much lower than that of thecytotoxin,the rosette inhibition of ALG mainly affected the cell surface.The effectwas blind rather than cytotoxic in nature.