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OBJECTIVE: To optimize water extraction technology of Feng-Moutan Cortex. METHODS: L9 (34) orthogonal design was used to optimize soaking time, solid-liquid ratio, extraction time and extraction times in water extraction technology of Feng-Moutan Cortex using comprehensive scores for the peak area percentage and extract yield of 8 ingredients in Feng-Moutan Cortex as gallic acid, paeoniflorin, catechin, paeoniflorin, benzoic acid, benzoyloxypaeoniflorin, benzoylpaeoniflorin and paeonol after normalization as indexes. Verification test was conducted. RESULTS: The optimal water extraction technology was that 20-fold water, soaking for 1 h, extracting twice, 2 h each time. Results of verification test showed that comprehensive scores of 3 times of tests were 65. 98, 68. 85 and 69. 25, respectively. RSDs of each index were lower than 5％ (n=3). CONCLUSIONS: Established comprehensive scoring method can reflect the relative contents of effective components in Feng-Moutan Cortex comprehen- sively, so that optimized extraction technology is reasonable and feasible.
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Objective To explore the effects of different processes on the contents of three alkaloid ingredients in Coridalis Rhizoma.Methods The contents of three alkaloid ingredients (protopine, dehydrocorydaline, tetrahydropalmatine) were determined by HPLC. Agilent HC-C18(2) column (4.6 mm × 250 mm, 5μm) was set at 35℃; acetonitrile-0.1% phosphoric acid solution (triethylamine adjusted pH 5.3) (26:74) was set as mobile phase; the flow rate was 1.0 mL/min and detection wavelength was set at 280 nm; the injection volume was 10μL. The three components were separated well under these chromatographic conditions. The linear relationship between the mass of each component and the peak area was good (r2≥0.9996). The average recoveries were 100.31%, 99.34%, and 99.34%, respectively (RSD≤2.70%).Results The contents of protopine, dehydrocorydaline, tetrahydropalmatine and the total contents of three alkaloids were higher than other groups in large size samples (diameter of fresh products was 2.0–3.0 cm) which were boiled 5–6 minutes, the medium size samples (diameter of fresh products was 1.5–1.9 cm) which were boiled 2–4 minutes and the small size samples (diameter of fresh products was 0.7–1.4 cm) which were boiled 1–2 minutes. The contents of alkaloid in boiled process were lower than steamed process.Conclusion The Results of study provide an experimental basis for origin processing of Coridalis Rhizoma.
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Objective To investigate effects of different processing methods on the contents of oleanolic acid and ursolic acid in chaenomeles medicinal materials. Methods The contents of oleanolic acid and ursolic acid in samples processed by different processing methods in producing area were determined by HPLC with Agilent HC-C18 (2) chromatographic column (4.6 mm×250 mm, 5 μm), mobile phase of methanol-water-acetic acid-triethylamine (89∶11∶0.1∶0.05), detection wavelength of 210 nm, column temperature at 25 ℃, the flow rate of 1.0 mL/min. Results The total contents of oleanolic acid and ursolic acid in the samples prepared through 4 different processing methods were 0.86%, 0.93%, 1.29%, and 1.47%, respectively, showing that different processing methods in producing area had a significant effect on contents of oleanolic acid and ursolic acid in chaenomeles medicinal materials. Conclusion This study provides a basis for processing methods of chaenomeles medicinal materials in producing area.
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Objective To research the best processing method of Rhizoma Atractylodis Macrocephalae stir-fried with bran. Methods L9(34) orthogonal table are decided with three factors:The amount of the adjuvant, heating temperature, heating time. According to the amount of water-soluble extractive, alcohol-soluble extractive and volatile oil, orthogonal design is applied to processing. Results The best processing method is:Rhizoma Atractylodis Macrocephalae were processed by stir-frying with 10 percent of bran for 5 min in a heat of 150 ℃. Conclusion The results can provide basis for establishing the quality standard of this crude drug.
