1.
Evanescence wave-based technology for the rapid and sensitive quantification of biological analytes.
J Allergy Clin Immunol
; 141(2): 817-820.e5, 2018 02.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29074455
2.
Biosens Bioelectron
; 26(4): 1631-7, 2010 Dec 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-20851593
RESUMO
An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.
