Multi-wavelength analyses of second-derivative spectra for rapid determination of acetaminophen in serum.

Measurement of acetaminophen by analysis of the second derivative of its spectrum is specific and sensitive. The method of extraction and the use of just one phosphate buffer as reagent makes this method very convenient. Readings are reliable from 10 to 1500 mg/L. A turnaround time of 20 min makes this method well suited for emergency cases. Precision and accuracy of the method are presented. Results are not biased by interferences, not even from N-acetylcysteine.

[Determination of urinary free cortisol by HPLC (high performance liquid chromatography) and UV detection]. / Dosage du cortisol libre urinaire par HPLC (High Performance Liquid Chromatography) et détection UV.

Using basal isocratic equipment of HPLC and only one step extraction the determination of urinary free cortisol is available in non specialized laboratory. Results are displayed about reliability and specificity of the method. An extraction coefficient of 97.8 per cent, a good practicability in connection with the use of C18 column chromatography and a reagent flow of 0.5 ml/min. enable a low cost of this determination. Reference values are established for a working adult population.