Thermopriming Induces Time-Limited Tolerance to Salt Stress.

Implementing sustainable crop protection practices is crucial to protect global harvests and ensure high-quality food supplies. While priming is an established method in seed production for the fortification of plants against various stresses, it is not yet a standard practice in transplant cultivation. Thus, we evaluated the long-term effects of thermopriming-a heat-based priming technique-on the growth, development, and fruit yield of tomato plants. Following a recovery period of about six weeks for thermoprimed plants without stress inducers, we subjected them to subsequent salt stress to ascertain the persistence of the priming effects. Additionally, we compared the efficacy of thermopriming with benzothiadiazole (BTH), a chemical elicitor, in enhancing plant resilience to abiotic stress. While BTH application negatively impacted both plant growth and fruit health, thermopriming showed no such adverse effects on these parameters. Instead, thermopriming initially enhanced the plant defense mechanisms by increasing the accumulation of protective phenols and flavonoids in the leaves. Interestingly, while thermopriming did not alter the response to salt stress, it notably strengthened the overall resilience of the plants. Our findings underscore both the potential and temporal constraints of thermopriming memory. Nonetheless, primed plants exhibited temporarily increased stress tolerance, offering a means to safeguard the offspring.

Heat-Induced Cross-Tolerance to Salinity Due to Thermopriming in Tomatoes.

Global plant production is challenged by unpredictable (a)biotic stresses that occur individually, simultaneously or staggered. Due to an increasing demand for environmentally friendly plant production, new sustainable, universal, and preventive measures in crop protection are needed. We postulate thermopriming as a suitable procedure that fulfills these requirements. Therefore, we performed thermopriming as a pre-conditioning on tomato transplants in combination with two subsequent salt stress treatments to evaluate their single and combined physiological effects on leaves and fruits with regard to plant performance, fruit yield and quality. We identified a cross-tolerance to salinity that was triggered by the preceding thermopriming treatment and resulted in an accumulation of phenols and flavonols in the leaves. Plant growth and fruit yield were initially delayed after the stress treatments but recovered later. In regard to fruit quality, we found an increase in carotenoid and starch contents in fruits due to thermopriming, while sugars and titratable acidity were not affected. Our results indicate that thermopriming can mitigate the impact of subsequent and recurrent stress events on plant performance and yield under production-like conditions.

Boosting leaf contents of rutin and solanesol in bio-waste of Solanum lycopersicum.

In tomato production, the accruing green biomass shows promising potential as source of health-promoting compounds, such as rutin and solanesol, that are of high interest due to their medicinal properties. Naturally, they accumulate in plants growing in suboptimal growing conditions, e.g. influenced by biotic and abiotic stressors. With the aim to evaluate the potential use of tomato residues as source, we analyzed both leaf metabolites during a complete cultivation cycle, while applying single and combined stresses practically realized in greenhouse production. In the late season, contents of both metabolites were significantly enhanced by nutrient deficit in combination with 2 °C colder nights for 4 weeks and prolonged for in total 9 weeks. Particularly, higher solanesol contents were achieved by salt stress and elevated temperature after one week, even stronger when combined with drought. At harvest, stressed plants consist of less green biomass reducing the overall economic potential. However, practicable abiotic stresses should be considered as potential tool to induce the accumulation of beneficial compounds. Extracting profitable metabolites from the green biomass of the model crop tomato supports the overall goal to promote sustainable approaches in horticultural production.

Phenotyping in Arabidopsis and Crops-Are We Addressing the Same Traits? A Case Study in Tomato.

The convenient model Arabidopsis thaliana has allowed tremendous advances in plant genetics and physiology, in spite of only being a weed. It has also unveiled the main molecular networks governing, among others, abiotic stress responses. Through the use of the latest genomic tools, Arabidopsis research is nowadays being translated to agronomically interesting crop models such as tomato, but at a lagging pace. Knowledge transfer has been hindered by invariable differences in plant architecture and behaviour, as well as the divergent direct objectives of research in Arabidopsis versus crops compromise transferability. In this sense, phenotype translation is still a very complex matter. Here, we point out the challenges of "translational phenotyping" in the case study of drought stress phenotyping in Arabidopsis and tomato. After briefly defining and describing drought stress and survival strategies, we compare drought stress protocols and phenotyping techniques most commonly used in the two species, and discuss their potential to gain insights, which are truly transferable between species. This review is intended to be a starting point for discussion about translational phenotyping approaches among plant scientists, and provides a useful compendium of methods and techniques used in modern phenotyping for this specific plant pair as a case study.