Therapeutic effect of mesenchymal stem cell therapy in the LVEF, LVEDV, and LVESV after myocardial infarction.

OBJECTIVES: The present study was aimed to investigate the therapeutic effect of mesenchymal Stem Cell Therapy in the left ventricular ejection fraction (LVEF), left ventricular enddiastolic volume (LVEDV), and  left ventricular endsystolic volume (LVESV) after myocardial infarction (MI). BACKGROUND: Previous investigations propose that stem cell therapy may help treat myocardial infarction (MI). However, there are controversial data from different studies. METHODS: We studied the relevant scientific literature available up to 2020. Comprehensive Meta-Analysis Software (CMAS) Version 2.0 were used for statistical analyses. Fixed or random-effect model was used to identify the weighted mean difference (WMD) with 95% confidence intervals (CI). The statistically significant level used for interpreting publication bias was less than 0.05. RESULTS: We identified 30 studies that met the inclusion criteria. In the overall pooled estimate, cell therapy had an effect on the LVEF change from baseline to follow-up (WMD: 2.98 mL, 95% CI: 1.66 to 4.29). The pooled WMD was found to be -4.16 (95% CI: -7.91 to -0.40) and -5.62 (95% CI: -9.20 to -2.00), for LVEDV, and LVESV, respectively. Thus, reduction in LVEDV and LVESV were significant in the treatment group participants. CONCLUSIONS: The present systematic review indicated that cell therapy in patients, who have MI could be effective and applicable clinically (Tab. 3, Fig. 7, Ref. 48). Text in PDF www.elis.sk Keywords: myocardial infarction, stem cell, systematic review, randomized clinical trials.

Uropathogenic Escherichia coli in the high vaginal swab samples of fertile and infertile women: virulence factors, O-serogroups, and phenotyping and genotyping characterization of antibiotic resistance.

Transmission of urinary tract infections into the reproductive system is unavoidable. The present research was performed to assess the distribution of virulence genes, O-serogroups and antibiotic resistance properties of uropathogenic Escherichia coli (UPEC) strains isolated from the high vaginal swab samples of fertile and infertile women. A total of 460 high vaginal swab samples were taken from fertile and infertile women. Distribution of virulence factors and serogroups and antibiotic resistance properties of the E. coli isolates were assessed. Sixty-five out of 460 (14.13%) swab samples were positive for E. coli. Prevalences of E. coli in samples taken from fertile and infertile women were 13.63% and 14.58%, respectively. O1 (7.69%), O2 (6.15%) and O6 (6.15%) were the most frequently detected serogroups. The most frequently detected virulence genes were sfa (72.72%), afa (72.72%), cnf1 (72.72%) and fim (72.72%). The most commonly detected antibiotic-resistance genes were tetA (95.45%), CITM (88.63%), aac(3)-IV (86.36%) and sul1 (72.72%). UPEC strains harboured the highest prevalence of resistance against tetracycline (88.63%), ampicillin (79.54%), gentamicin (77.27%) and enrofloxacin (52.27%). Seventeen out of 26 (65.38%) UPEC strains isolated from infertile women were resistant toward more than ten antibiotic agents. Infertile women with a history of urinary tract infections had the higher prevalence of UPEC strains and also the other characters. High prevalence of the virulent and resistant UPEC strains in the high vaginal part of the infertile women with a history of urinary tract infections may show an important role of these pathogens as causes of female infertility. However, further research is required to confirm this hypothesis.

Full-Genome Sequences of Two Newcastle Disease Virus Strains Isolated in West Java, Indonesia.

The full-genome sequences of strains chicken/Indonesia/Cilebut/010WJ/2015 and chicken/Indonesia/ITA/012WJ/1951, isolated in West Java, Indonesia, in 2015 and 1951, respectively, were examined. Chicken/Indonesia/Cilebut/010WJ/2015 (genotype VII) caused a 2015 disease outbreak in Indonesia, and chicken/Indonesia/ITA/012WJ/1951 (genotype VI) is used as a standard strain for challenge in Newcastle disease virus (NDV) vaccine trials.

The synthesis and characterization of benzotriazole-based cationic surfactants and the evaluation of their corrosion inhibition efficiency on copper in seawater.

This study aims at preparing three cationic surfactants based on benzotriazole and evaluating their efficiencies as corrosion inhibitors for copper electrodes in seawater using different electrochemical techniques (potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM)). FTIR and 1H NMR spectroscopic techniques confirmed the chemical structures of the as-prepared cationic compounds. The inhibition efficiency increased with the increase the concentration of the as-prepared compounds in the solution. The curves of the potentiodynamic polarization and the plots of EIS techniques show that the performance of all investigated compounds as mixed type. The standard free energy values imply that the three as-prepared compounds show physicochemical adsorption and obey the Langmuir adsorption model. AFM technique observed the reduction in the surface roughness due to the protective film formed on the copper surface. Finally, computational calculations show a great correlation with the experimental results due to the electron-donating effect.

Understanding the adsorption performance of two glycine derivatives as novel and environmentally safe anti-corrosion agents for copper in chloride solutions: experimental, DFT, and MC studies.

The inhibition impacts of two non-toxic glycine derivatives, namely, bicine (N,N-bis(2-hydroxyethyl)glycine) and tricine (N-(tri(hydroxymethyl)methyl) glycine) on copper corrosion were investigated in 3.5% NaCl solutions. Surprisingly, there is no report on using bicine and/or tricine as corrosion inhibitors for Cu and its alloys in a seawater-like environment. The effects of bicine and tricine on the corrosion behavior of Cu in 3.5% NaCl were examined using the open circuit potential, Tafel polarization, and AC spectroscopy (EIS) techniques. The corrosion rate decreased as a function of the inhibitor dose. The Tafel and EIS parameters showed that the inhibitors decreased both the anodic and cathodic corrosion currents and inhibited the charge transfer process by adsorption on the Cu surface. The inhibition property was attributed to the adsorption of inhibitor molecules with the Langmuir model. Tricine showed a superior inhibition performance of more than 98% at a concentration of ∼5 mmol L-1. The free energy of adsorption data revealed physical adsorption. The outcomes of Monte Carlo simulations and theoretical studies well supported the experimental data.

Free amino acids in stimulated and unstimulated whole saliva: advantages or disadvantages.

This study determines the mean concentrations of free amino acids in stimulated and unstimulated whole saliva in healthy young adults. Standardised salivary amino acids as a substitute for their counterpart in blood, searched for the source of free amino acids in saliva, the probable correlation between particular amino acids with caries experience. Stimulated and unstimulated whole saliva were collected by the draining method in 31 dental students. Saliva was purified, and amino acids were separated by high-performance liquid chromatography. DMFT scores were recorded, and the relation of amino acids to caries experience was explored by generalised linear model. Almost all amino acids had higher concentration in unstimulated whole saliva than in stimulated saliva. The normal range of amino acids (95% CI) and their natural logarithm were defined. There was a significant relationship between caries experience and threonine (P < 0·008), citrulline (P < 0·023) and ornithine (P < 0·001) as a detrimental factor, whereas serin (P < 0·026), glutamine (P < 0·015) and phenylalanine (P < 0·014) had an inhibiting effect on caries. However, in comparison, salivary flow rate (P < 0·013) was a more preventive factor than amino acids. Amino acids in saliva contribute as a marker, instead of their counterpart in blood. Unstimulated saliva had higher concentration of amino acids. Amino acids have different impact on caries and may be one of underlying risk factors for caries experience.