RESUMO
ization and unification of diseases of the oral mucosa in patients who underwent Covid-19, in order to increase the effectiveness of qualified dental care for this group of people in a pandemic and create conditions for continuous professional development of dentists. 42 patients were attended, who came through Covid-19, aged 36 to 70 years, who applied independently or were referred by doctors of private clinics in the city of Ternopil. The gender distribution of patients was 19 males and 23 females. Clinical examination of patients with acute respiratory viral infection Сovid-19, at the stage of subjective methods, revealed the following complaints: loss of taste was found in 38 people (90%) patients, salivation disorders (dry mouth or hypersalivation) - in 40 people (95%) patients, burning sensation, discomfort and «creeping ants¼ in the tongue or other anatomical components of the oral mucosa in 29 people (69%) patients. Complaints about the integrity of the oral mucosa and the associated pain were associated with pain in 13 people (31%). Patients diagnosed with Covid-19 were diagnosed with glossodynia 7 individuals, (17%), candidal stomatitis 12 individuals, (28%), herpetic stomatitis 5 individuals, (12%), and chronic recurrent aphthous stomatitis 8 individuals, (19%), desquamative glossitis in 10 people (24%). In some clinical cases, 10 people (24%) observed a combination of nosological units. The obtained results substantiate the development of the necessary algorithm of dental examination of dental data of patients in a pandemic.
Assuntos
COVID-19 , Mucosa Bucal , Odontólogos , Feminino , Humanos , Masculino , Pandemias , SARS-CoV-2 , Organização Mundial da SaúdeRESUMO
The protocol for production of full-size cDNA using SuperScript Full-Length cDNA Library Construction Kit II (Invitrogen) was tested and high quality cDNA library from meristematic tissue of finger millet panicle (Eleusine coracana (L.) Gaertn) was created. The titer of obtained cDNA library comprised 3.01 x 10(5) CFU/ml in avarage. In average the length of cDNA insertion consisted about 1070 base pairs, the effectivity of cDNA fragment insertions--99.5%. The selective sequencing of cDNA clones from created library was performed. The sequences of cDNA clones were identified with usage of BLAST-search. The results of cDNA library analysis and selective sequencing represents prove good functionality and full length character of inserted cDNA clones. Obtained cDNA library from meristematic tissue of finger millet panicle represents good and valuable source for isolation and identification of key genes regulating metabolism and meristematic development and for mining of new molecular markers to conduct out high quality genetic investigations and molecular breeding as well.
Assuntos
DNA Complementar/genética , DNA de Plantas/genética , Eleusine/genética , Biblioteca Gênica , Meristema/genética , Sequência de Bases , Clonagem Molecular , Primers do DNA/genética , DNA de Plantas/isolamento & purificação , Escherichia coli/genética , Dados de Sequência Molecular , RNA de Plantas/genética , RNA de Plantas/isolamento & purificaçãoRESUMO
Agrobacterium tumefaciens was used to transform fiber flax with the pBITUBA8 plasmid carrying the mutant alpha-tubulin gene imparting resistance to dinitroaniline herbicides and the nptII selective marker gene imparting resistance to canamycin. The transformants were selected in parallel on media containing canamycin and trifluralin (a dinitroaniline herbicide). The transgenic nature of the resultant regenerants resistant to dinitroaniline herbicides was confirmed by means of Southern blotting and polymerase chain reaction (PCR) analysis using specific probes for the ntpll gene and the gene of alpha-tubulin.
Assuntos
Agrobacterium tumefaciens , Compostos de Anilina/farmacologia , Linho/metabolismo , Resistência a Herbicidas , Herbicidas/farmacologia , Mutação , Proteínas de Plantas/biossíntese , Transformação Genética , Tubulina (Proteína)/biossíntese , Linho/genética , Linho/crescimento & desenvolvimento , Proteínas de Plantas/genética , Tubulina (Proteína)/genéticaRESUMO
Biolistic transformation of soybean by construction carrying a mutant alpha-tubulin gene as selective marker gene conferring resistance to dinitroaniline herbicides, and by additional construction pAHTUBI carrying a full-length barley beta-tubulin gene for correct co-expression of exogeneous tubulin in cells of transgenic soybean lines has been realized. It was established that 10 microM trifluralin is the most optimal selective concentration to pick up transformed soybean lines. Transgenic nature of offained regenerants was confirmed by Southern blotting hybridization using specific probe to selective alpha-tubulin gene.
Assuntos
Glycine max/genética , Herbicidas/farmacologia , Plantas Geneticamente Modificadas , Transformação Genética/genética , Trifluralina/farmacologia , Tubulina (Proteína)/genética , Genes de Plantas , Marcadores Genéticos/genética , Resistência a Herbicidas/genética , Glycine max/crescimento & desenvolvimentoRESUMO
Plants of the Brassicaceae family are important oil, vegetable and feed crops. The review is devoted to the latest achievements in genetic engineering of plants from this family. Results concerning development of effective methods both of Agrobacteium-mediated transformation and of direct gene uptake are considered. Particularly, possibilities of plant genetic modification with the aim to improve agronomically and commercially important traits are stressed. Problems of biologically safe introduction of transgenic plants into agricultural production are discussed.
Assuntos
Biotecnologia/tendências , Brassicaceae/crescimento & desenvolvimento , Engenharia Genética/tendências , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Transformação Genética , Brassicaceae/genética , Engenharia Genética/métodos , Plantas Geneticamente Modificadas/genéticaRESUMO
The control of epidermal cell fate is a complex molecular process and requires the regulatory activity of different transcription factors. Here, we describe the isolation of a member of the Arabidopsis MYB transcription factor family, AtMYB23, that is involved in trichome development. Expression of the AtMYB23 gene under the control of the viral CaMV 35S promoter causes the development of ectopic trichomes. The formation of ectopic trichomes depends on TRANSPARENT TESTA GLABRA1 but not on GLABRA1. The absence of the negative regulator TRIPTYCHON leads to branching of the ectopic trichomes on cotyledons and the formation of ectopic trichomes in the leaf subepidermal cell layer. The CaMV 35S promoter-controlled expression of AtMYB23 can partially rescue the glabra1 mutant phenotype. Together, the presented data indicate that the AtMYB23 gene has partially overlapping functions with GLABRA1 in controlling the initiation of trichome development.
Assuntos
Proteínas de Arabidopsis , Arabidopsis/genética , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Genes myb/genética , Proteínas de Plantas/biossíntese , Proteínas de Plantas/genética , Proteínas Proto-Oncogênicas c-myb , Arabidopsis/ultraestrutura , Diferenciação Celular , DNA Complementar/metabolismo , Vetores Genéticos , Microscopia Eletrônica de Varredura , Fenótipo , Proteínas de Plantas/metabolismo , Regiões Promotoras Genéticas , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transformação GenéticaRESUMO
The procedure for genetic transformation of two spring and one winter rapeseed cultivars was developed. No-paline strains of Agrobacterium tumefaciens GV3101 and EHA105 were shown to be preferable for gene transfer, as compared to the octopine strain GV2260. With two types of plant explants, the segments of hypocotyls and cotyledons, transformation was successful; however, its efficiency was somewhat higher with the fragments of hypocotyls. Analysis of regenerated plants by PCR and Southern blotting confirmed the presence of the nptII and nisA genes in transformants. RNA analysis by Northern blotting showed expression of the nisA gene in transformed shoots. The transgenes were inherited in T2 as Mendelian traits. The effect of biotic and abiotic factors on the efficiency of genetic transformation in rapeseed is discussed.
Assuntos
Agrobacterium tumefaciens/genética , Brassica/genética , Plantas Geneticamente Modificadas/genética , Transformação Genética , Sequência de Bases , Northern Blotting , Southern Blotting , Primers do DNA , Genes de PlantasRESUMO
Intercomparison of EPR-dosimetric techniques using tooth enamel had been performed in order to check whether the results produced by different laboratories are consistent and accurate. Participants were supposed to evaluate doses applied to pulverized enamel samples, using routine techniques from their laboratories. The intercomparison has demonstrated a great variety of methods used for dose reconstruction. Peculiarities of experimental approaches are discussed systematically in terms of procedure for recording the EPR-spectra, determination of the amplitude of the radiation induced signal, determination of the dose, and error propagation.
Assuntos
Esmalte Dentário/efeitos da radiação , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Radiometria/métodos , Esmalte Dentário/química , Espectroscopia de Ressonância de Spin Eletrônica/estatística & dados numéricos , Estudos de Avaliação como Assunto , Radicais Livres/análise , Radicais Livres/efeitos da radiação , Humanos , Laboratórios , Tolerância a Radiação , Radiometria/estatística & dados numéricosRESUMO
It is shown that metamorphic modifications in tooth enamel have an essential influence on the results of EPR dosimetry. The metamorphic modifications in minerals of biological origin proceed more quickly than in usual natural minerals. The approaches which at present are applied for reconstruction of doses connected with Chernobyl accident need additional investigation.
