RESUMO
The transport medium Multi-Microbe Media (M4) was evaluated prospectively by culture and direct enzyme-linked immunosorbent assay (ELISA) for detection of herpes simplex virus from 473 specimens. In addition, 377 specimens in Bartels Viral Transport Medium were evaluated. By using culture as a "gold standard," the ELISA sensitivity was approximately 85%, while the specificities exceeded 96% for both media.
Assuntos
Meios de Cultura , Ensaio de Imunoadsorção Enzimática , Simplexvirus/isolamento & purificação , Antígenos Virais/análise , Herpes Simples/diagnóstico , Humanos , Estudos Prospectivos , Simplexvirus/imunologiaRESUMO
The aim of the present work was to determine and analyse the nutritional status and plasma lipid levels in diabetic and control subjects dependent on the type of lipidemia. The investigations involved four groups: controls with normal lipidemia, diabetic (NIDDM) subjects with normal lipidemia, persons with hyperlipidemia type IV, and without diabetes mellitus, and diabetic subjects with hyperlipidemia type IV. The values for the concentrations of plasma triacylglycerol, cholesterol, phospholipids and HDL-phospholipids were significantly higher in both diabetic groups than in controls. The values for the relative amount and concentration of HDL-cholesterol were significantly lower in diabetics than in the control groups.
