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1.
Sci Rep ; 7: 44613, 2017 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-28300220

RESUMO

Dietary gluten causes severe disorders like celiac disease in gluten-intolerant humans. However, currently understanding of its impact in tolerant individuals is limited. Our objective was to test whether gliadin, one of the detrimental parts of gluten, would impact the metabolic effects of an obesogenic diet. Mice were fed either a defined high-fat diet (HFD) containing 4% gliadin (n = 20), or a gliadin-free, isocaloric HFD (n = 20) for 23 weeks. Combined analysis of several parameters including insulin resistance, histology of liver and adipose tissue, intestinal microbiota in three gut compartments, gut barrier function, gene expression, urinary metabolites and immune profiles in intestinal, lymphoid, liver and adipose tissues was performed. Mice fed the gliadin-containing HFD displayed higher glycated hemoglobin and higher insulin resistance as evaluated by the homeostasis model assessment, more hepatic lipid accumulation and smaller adipocytes than mice fed the gliadin-free HFD. This was accompanied by alterations in the composition and activity of the gut microbiota, gut barrier function, urine metabolome, and immune phenotypes within liver and adipose tissue. Our results reveal that gliadin disturbs the intestinal environment and affects metabolic homeostasis in obese mice, suggesting a detrimental effect of gluten intake in gluten-tolerant subjects consuming a high-fat diet.


Assuntos
Microbioma Gastrointestinal , Gliadina/administração & dosagem , Homeostase , Adipócitos/patologia , Tecido Adiposo/metabolismo , Tecido Adiposo/patologia , Animais , Tamanho Celular , Dieta Hiperlipídica , Regulação da Expressão Gênica , Glucose/metabolismo , Inflamação/patologia , Mediadores da Inflamação/metabolismo , Intestinos/microbiologia , Intestinos/patologia , Metabolismo dos Lipídeos , Fígado/metabolismo , Masculino , Metaboloma , Camundongos Endogâmicos C57BL , Modelos Biológicos , Fenótipo , Urina
2.
Mol Nutr Food Res ; 61(5)2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28012235

RESUMO

SCOPE: Omega-6 (n-6) PUFA-rich diets are generally considered obesogenic in rodents. Here, we examined how long-term intake of a high-fat/high-sucrose (HF/HS) diet based on safflower oil affected metabolism, inflammation, and gut microbiota composition. METHODS AND RESULTS: We fed male C57BL/6J mice a HF/HS diet based on safflower oil-rich in n-6 PUFAs-or a low-fat/low-sucrose diet for 40 wk. Compared to the low-fat/low-sucrose diet, intake of the safflower-based HF/HS diet only led to moderate weight gain, while glucose intolerance developed at week 5 prior to signs of inflammation, but concurrent with increased levels of linoleic acid and arachidonic acid in hepatic phospholipids. Intake of the HF/HS diet resulted in early changes in the gut microbiota, including an increased abundance of Blautia, while late changes coincided with altered inflammatory profiles and increased fasting plasma insulin. Analysis of immune cells in visceral fat and liver revealed no differences between diets before week 40, where the number of immune cells decreased in the liver of HF/HS-fed mice. CONCLUSION: We suggest that a diet-dependent increase in the n-6 to omega-3 (n-3) PUFA ratio in hepatic phospholipids together with gut microbiota changes contributed to early development of glucose intolerance without signs of inflammation.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Sacarose Alimentar/efeitos adversos , Microbioma Gastrointestinal , Intolerância à Glucose/sangue , Óleo de Cártamo/administração & dosagem , Animais , Gorduras na Dieta/administração & dosagem , Sacarose Alimentar/administração & dosagem , Ácidos Graxos Ômega-3/administração & dosagem , Ácidos Graxos Ômega-3/sangue , Ácidos Graxos Ômega-6/administração & dosagem , Ácidos Graxos Ômega-6/sangue , Trato Gastrointestinal/microbiologia , Intolerância à Glucose/etiologia , Inflamação/sangue , Inflamação/etiologia , Fígado/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL
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