Effect of phosphorus sources on growth and cadmium accumulation in wheat under different soil moisture levels.

Both cadmium (Cd) toxicity and water limited stress in crop plants are serious concerns worldwide while little is known about the impact of various phosphorus (P) sources on Cd accumulation in cereals especially under water limited stress. A study was conducted to explore the efficiency of three frequently available P fertilizers on Cd accumulation in wheat under different soil moisture levels. Three different P sources including diammonium phosphate (DAP), single super phosphate (SSP), and nitrophos (NP) were applied in the soil with three levels (0, 50 and 100 mg/kg). The drought stress was applied to half treatments during the latter growth stages of wheat and plants were harvested at maturity. The results demonstrated that water-limited stress decreased the growth and yield of plants than respective treatments without water stress. P supply increased the growth of wheat irrespective of water-limited stress. The effect on growth and yield varied with the sources and levels of P and maximum effects was observed in DAP treatment (100 mg/kg). The P amendments enhanced the leaf photosynthesis and activities of SOD, POD, CAT and decreased the leaf oxidative burst. Water limited stress enhanced the Cd concentrations in shoots, roots, and grains whereas P amendments minimized the Cd concentrations and enhanced the P concentrations in these parts of plants. The results obtained demonstrated that P supply in the form of DAP might be effective in minimization of Cd in grains and can be used for safe cultivation of metal-contaminated soils.

Plant Growth-Promoting Rhizobacteria Eliminate the Effect of Drought Stress in Plants: A Review.

Plants evolve diverse mechanisms to eliminate the drastic effect of biotic and abiotic stresses. Drought is the most hazardous abiotic stress causing huge losses to crop yield worldwide. Osmotic stress decreases relative water and chlorophyll content and increases the accumulation of osmolytes, epicuticular wax content, antioxidant enzymatic activities, reactive oxygen species, secondary metabolites, membrane lipid peroxidation, and abscisic acid. Plant growth-promoting rhizobacteria (PGPR) eliminate the effect of drought stress by altering root morphology, regulating the stress-responsive genes, producing phytohormones, osmolytes, siderophores, volatile organic compounds, and exopolysaccharides, and improving the 1-aminocyclopropane-1-carboxylate deaminase activities. The use of PGPR is an alternative approach to traditional breeding and biotechnology for enhancing crop productivity. Hence, that can promote drought tolerance in important agricultural crops and could be used to minimize crop losses under limited water conditions. This review deals with recent progress on the use of PGPR to eliminate the harmful effects of drought stress in traditional agriculture crops.

Exogenous application of gibberellic acid and silicon to promote salinity tolerance in pea (Pisum sativum L.) through Na+ exclusion.

Salinity is a worldwide problem limiting the plant growth and risking food security. This study was conducted to examine exogenous application of silicon (Si), gibberellic acid (GA3) upon the ion transport, growth, yield, and antioxidant enzymes activities of pea plant in saline conditions. Two pea varieties Meteor-FSD and Samrina Zard were pre-treated with GA3 (10-4 M) for 12 h. Plants were allowed to grow with or without silicon in washed silica sand. Ten days old seedlings were shifted in pots with 10 kg soil. Twenty-five days old plants were exposed to 0 and 5 dS m-1 sodium stress. Results showed that exogenous application of GA3 + Si was the best treatment for increasing plant biomass and yield in the presence and absence of NaCl. Furthermore, application of Si or GA3 enhanced chlorophyll content in the leaves, thereby increasing the net assimilation rate of pea varieties under NaCl stress by increasing the antioxidant enzyme activity. Treatment of Si alone or in combination with GA3 significantly reduced Na+ movement in both pea varieties. Results showed that Si has more prominent role than GA3 alone to build-up high plant biomass, yield, soluble protein content and reduction of Na+ transport. Samrina Zard variety showed higher yield, shoot and root dry weight as compared to Meteor-FSD variety in presence and absence of salt. It was concluded that Si can be used as a nutrient for pea under saline or non-saline conditions. Moreover, application of GA3 has a potential role for increasing salinity tolerance, mostly in sensitive pea varieties.

Investigating the degradation behavior of Cypermethrin (CYP) and Chlorpyrifos (CPP) in peach orchard soils using organic/inorganic amendments.

Reducing toxic effects of pesticide residues in agricultural soils through organic amendments is an eco-friendly technique. Cypermethrin (CYP) and Chlorpyrifos (CPP) are widely used pesticides in peach growing orchards in Swat valley of Pakistan. The aim of the current study was to investigate the degradation behavior of CYP and CPP in soil by the application of different combination of organic/inorganic amendments. A total of 36 soil samples were used in the current incubation study which was collected from 4 peach orchards in district Swat, Khyber Pakhtunkhwa (KPK), Pakistan. Different amendments including urea, farm yard manure (FYM) and saprofil were applied alone and in various combinations. The initial concentrations of CYP and CPP in the tested soil was range from 0.94 to 4.8 mg kg-1 and 0.024 to 4.12 to mg kg-1. Soil samples were taken at 5, 15, 30 and 45 days after exposure to different treatments. The extraction of pesticides from soils was done through quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method. Soils amended with urea, FYM and saprofil individually and in combinations significantly reduced the concentrations of CYP and CPP. However, the concentration of CYP (24.6) and CPP (27.0) in soil showed higher reduction through the application of FYM. While the concentrations of CYP and CPP were declined with the 5, 15, 30 and 45 days intervals, however, reduction at day 30 and 45 was faster for CYP (16.7 to 8.46) than CPP (20.2 to 12.3). At day 5 and 15, the CYP (42.5 to 30.7) was slightly lower than CPP (42.9 to 32.7).The highest half-life value (t ½) of CYP was in control treatment (32 days) and the shortest was soil amended with FYM (18.6 days). While the longest half-life value (t ½) of CPP was maximum in control treatment (42 days) and the minimum was in FYM (22 days). Based on our findings, it was concluded that soil application of FYM is recommended for the degradation of CYP and CPP.

Mechanistic elucidation of germination potential and growth of Sesbania sesban seedlings with Bacillus anthracis PM21 under heavy metals stress: An in vitro study.

Soils contaminated with heavy metals such as Chromium (Cr) and Cadmium (Cd) severely impede plant growth. Several rhizospheric microorganisms support plant growth under heavy metal stress. In this study, Cr and Cd stress was applied to in vitro germinating seedlings of a Legume plant species, Sesbania sesban, and investigated the plant growth potential in presence and absence of Bacillus anthracis PM21 bacterial strain under heavy metal stress. The seedlings were exposed to different concentrations of Cr (25-75 mg/L) and Cd (100-200 mg/L) in Petri plates. Growth curve analysis of B. anthracis PM21 revealed its potential to adapt Cr and Cd stress. The bacteria supported plant growth by exhibiting ACC-deaminase activity (1.57-1.75 µM of α-ketobutyrate/h/mg protein), producing Indole-3-acetic acid (99-119 µM/mL) and exopolysaccharides (2.74-2.98 mg/mL), under heavy metal stress condition. Analysis of variance revealed significant differences in growth parameters between the seedlings with and without bacterial inoculation in metal stress condition. The combined Cr+Cd stress (75 + 200 mg/L) significantly reduced root length (70%), shoot length (24%), dry weight (54%) and fresh weight (57%) as compared to control. Conversely, B. anthracis PM21 inoculation to seedlings significantly increased (p ≤ 0.05) seed germination percentage (5%), root length (31%), shoot length (23%) and photosynthetic pigments (Chlorophyll a: 20%; Chlorophyll b: 16% and total chlorophyll: 18%), as compared to control seedlings without B. anthracis PM21 inoculation. The B. anthracis PM21 inoculation also enhanced activities of antioxidant enzymes, including superoxide dismutase (52%), peroxidase (66%), and catalase (21%), and decreased proline content (56%), electrolyte leakage (50%), and malondialdehyde concentration (46%) in seedlings. The B. anthracis PM21 inoculated seedlings of S. sesban exhibited significantly high (p ≤ 0.05) tissue deposition of Cr (17%) and Cd (16%) as compared to their control counterparts. Findings of the study suggested that B. anthracis PM21 endured metal stress through homeostasis of antioxidant activities, and positively impacted S. sesban growth and biomass. Further experiments in controlled conditions are necessary for investigating phytoremediation potential of S. sesban in metal-contaminated soils in presence of B. anthracis PM21 bacterial strain.

Unlocking the potential of plant growth-promoting rhizobacteria on soil health and the sustainability of agricultural systems.

The concept of soil health refers to specific soil properties and the ability to support and sustain crop growth and productivity, while maintaining long-term environmental quality. The key components of healthy soil are high populations of organisms that promote plant growth, such as the plant growth promoting rhizobacteria (PGPR). PGPR plays multiple beneficial and ecological roles in the rhizosphere soil. Among the roles of PGPR in agroecosystems are the nutrient cycling and uptake, inhibition of potential phytopathogens growth, stimulation of plant innate immunity, and direct enhancement of plant growth by producing phytohormones or other metabolites. Other important roles of PGPR are their environmental cleanup capacities (soil bioremediation). In this work, we review recent literature concerning the diverse mechanisms of PGPR in maintaining healthy conditions of agricultural soils, thus reducing (or eliminating) the toxic agrochemicals dependence. In conclusion, this review provides comprehensive knowledge on the current PGPR basic mechanisms and applications as biocontrol agents, plant growth stimulators and soil rhizoremediators, with the final goal of having more agroecological practices for sustainable agriculture.

Residual effects of biochar and phosphorus on growth and nutrient accumulation by maize (Zea mays L.) amended with microbes in texturally different soils.

The purpose of study was to examine the residual effects of two types of biochar amendments, two phosphorus (P) fertilizer levels, phosphorus solubilizing bacteria (PSB) and arbuscular mycorrhizal fungs (AMF) on plant growth, nutrients absorption and root architecture of Zea mays L. in texturally different soils. Biochar signficantly increased nutrients absorption and plant biomass production with P-fertilization and microbial inoculantion. Texturally different soils enhanced the plant biomass and nutrients absorption in their independent capacity on addition of biochar, microbial inoculants and P-fertilization. It was shown that mycorrhizal inoculation had positive influence on plant root and shoot biomass in both soils irrespective to the biochar type used. Root colonization was notably increased in biochar + mycorrhizae (B + M) inocultaed plants. It was shown that mycorrhizal inoculation had positive influence on nutrients absorption by plant roots and it had high content of P, potassium, calcium and magnesium in plants at all biochar and P levels. Without P fertilization, biochar amendments significantly promoted shoot P content and root colonization. The P application significantly influenced soil microbial activity in terms of nutrient concentration and plant growth. Root attributes were significantly inclined by microbial inoculation. Residual effects of biochar and P significantly enhanced the nutreints absorption and maize plant growth. Thus, we concluded that residual biochar and P fertilizer showed positive effects on nutrients absorption and maize plant growth promotion in differently textured soils. Microbial inoculants further stimulated the plant biomass production and nutrients absorption due to effective root colonization.

Assisted phytoremediation of chromium spiked soils by Sesbania Sesban in association with Bacillus xiamenensis PM14: A biochemical analysis.

Due to anthropogenic activities, chromium (Cr) contamination is ubiquitous with deleterious effects on plant and soil microbiota. Present study was designed to address beneficial effects of Bacillus xiamenensis PM14 on Sesbania sesban. Its physiological and biochemical attributes along with enhanced antioxidant enzyme activities under different levels of Cr toxicity (50, 100 and 200 mg kg-1) were evaluated. After harvesting at 50 days of sowing, plant growth attributes (root and shoot length, fresh and dry weight), physiological parameters (chlorophyll a, b and carotenoid content), antioxidant activities (superoxide dismutase, peroxidase and catalase), malondialdehyde content, electrolyte leakage, proline, relative water content and total Cr uptake in S. sesban were recorded. Experiment was statistically managed as complete randomized design (CRD). Results revealed that Cr stress reduced plant growth, relative water content at all levels of Cr contamination. However, inoculation of B. xiamenensis PM14 positively influence all parameters of S. sesban both under normal and stressed conditions. Inoculation of B. xiamenensis PM14 promoted plant growth (root length 17.08%, shoot length 28.36%) physiological attributes (chlorophyll a 55.26%, chlorophyll b 59.13%), antioxidant activities (superoxide dismutase 30.09%, peroxidase 6.96% and catalase 0.89%), relative water content 25.79%, enhanced total Cr uptake 47.33% and reduced proline 12.33%, malondialdehyde content 27.53% and electrolyte leakage 2.73% in S. sesban at 200 mg kg-1 Cr stress as compared to uninoculated plants grown under the same level of Cr. Our findings revealed first report of B. xiamenensis as phytoremediator and its inoculation on Sesbania plant. It also exposed dual effects of B. xiamenensis to ameliorate Cr stress along with improved plant growth and induced heavy metal stress tolerance in spiked soils.

Potential impact of biochar types and microbial inoculants on growth of onion plant in differently textured and phosphorus limited soils.

Non-renewable phosphorus (P) resources are intensively declining and recyclable P is high in demand for agricultural sector. Biochar as a renewable source of P and its physicochemical properties may improve the nutrients condition in the soil for plant availability. This study was designed to evaluate the interaction of biochar with soil microbes in differently textured and P-limited soils for P availability, root colonization and nutrient uptake by plants. Onion plants were grown in two differently textured soils with two types of biochar, with or without P application, three microbially inoculated treatments and uninoculated control. Plants were grown for 65 days and root-shoot biomass, nutrient concentration and mycorrhizal root colonization were analyzed. The WinRhizo was used to evaluate root attributes such as length, surface area and volume of roots. Biochar addition enhanced the nutrient uptake and plant biomass in the presence of P and microbial inoculants. Root colonization was notably increased in biochar + mycorrhizal inoculated plants. Biochar and soil type interactions may develop a unique behavior of nutrient uptake, root colonization, plant growth and root attributes. Biochar in combination with microbial inoculants could be considered a potentially renewable source of P fertilizer.

Effects of Rhizophagus clarus and biochar on growth, photosynthesis, nutrients, and cadmium (Cd) concentration of maize (Zea mays) grown in Cd-spiked soil.

Cadmium (Cd) toxicity in agricultural crops is a widespread problem. Little is known about biochar and arbuscular mycorrhizal fungi (AMF) effect on Cd concentration in maize plant either applied separately or in combination. Current study was performed to demonstrate effects of biochar and Rhizophagus clarus on plant growth, photosynthesis activity, nutrients (P, Ca, Mg, Fe, Cu, and Mn), and Cd concentration in maize grown in Cd-spiked soil. The alkaline soil was spiked by Cd factor at three levels: 0 (Cd 0), 5 (Cd 5), and 10 (Cd 10) mg/kg; biochar factor at two levels: 0 and 1%; and mycorrhizal inoculum factor at two levels: MF0 and MF1 (R. clraus). Plants were harvested after 70 days of seed germination, and various morphological and physiological parameters, as well as elemental concentration and root colonization, were recorded. Addition of biochar increased plant biomass by 21% (Cd 5) and 93% (Cd 10), MF1 enhanced by 53% (Cd 0) and 69% (Cd 10), while biochar + MF1 enhanced dry plant biomass by 70% (Cd 0) and 94% (Cd 10). Results showed maximum increase of 94% (Cd 10) in plant biomass was observed in Cd-spiked soil. Root colonization decreased proportionally by increasing Cd concentration and at Cd 10, colonization was 36.7% and 31.7% for MF1 and biochar + MF1 treatments, respectively. Besides that, addition of biochar enhanced root attributes (root length, volume, and surface area) by 34-58% compared to control in Cd 10. The MF1 increased these attributes by 11-78% while biochar + MF1 enhanced by 32-61% in Cd-spiked soil. However, biochar + MF1 neutralized Cd stress in maize plant for gaseous attributes (assimilation rate, transpiration rate, intercellular CO2, and stomatal conductance). The MF1 enhanced Cd concentration in plant as it was 3.32 mg/kg in Cd 5 and 6.73 mg/kg in Cd 10 treatments while addition of biochar phytostabilized Cd and reduced its concentration in plants by 2.0 mg/kg in Cd 5 and 4.27 mg/kg in Cd 10. The biochar + MF1 had 2.9 mg/kg and 4.8 mg/kg Cd concentration in Cd 5 and Cd 10 plants, respectively. Phosphorus concentration was augmented in shoots (up to 26%) and roots (up to 20%) of maize plant in biochar-amended soil than control plants. In biochar + MF1, concentration of P was 1.01% and 0.73% in Cd 5 and Cd 10, respectively. It is concluded that biochar + MF1 treatment enhances plant biomass while addition of sole biochar reduced Cd uptake, slightly indifferent to earlier treatment.

Biodegradation of chlorpyrifos by bacterial genus Pseudomonas.

Chlorpyrifos is an organophosphorus pesticide commonly used in agriculture. It is noxious to a variety of organisms that include living soil biota along with beneficial arthropods, fish, birds, humans, animals, and plants. Exposure to chlorpyrifos may cause detrimental effects as delayed seedling emergence, fruit deformities, and abnormal cell division. Contamination of chlorpyrifos has been found about 24 km from the site of its application. There are many physico-chemical and biological approaches to remove organophosphorus pesticides from the ecosystem, among them most promising is biodegradation. The 3,5,6-trichloro-2-pyridinol (TCP) and diethylthiophosphate (DETP) as primary products are made when chlorpyrifos is degraded by soil microorganisms which further break into nontoxic metabolites as CO(2), H(2)O, and NH(3). Pseudomonas is a diversified genus possessing a series of catabolic pathways and enzymes involved in pesticide degradation. Pseudomonas putida MAS-1 is reported to be more efficient in chlorpyrifos degradation by a rate of 90% in 24 h among Pseudomonas genus. The current review analyzed the comparative potential of bacterial species in Pseudomonas genus for degradation of chlorpyrifos thus, expressing an ecofriendly approach for the treatment of environmental contaminants like pesticides.

Differential effects of cadmium and chromium on growth, photosynthetic activity, and metal uptake of Linum usitatissimum in association with Glomus intraradices.

The current study was aimed at analyzing the differential effects of heavy metals (cadmium and chromium) and mycorrhizal fungus; Glomus intraradices on growth, chlorophyll content, proline production, and metal accumulation in flax plant (Linum usitatissimum L.). Heavy metal accumulation rate in flax varied from 90 to 95 % for Cd and 61-84 % for Cr at a concentration range of 250 to 500 ppm for both metals in 24 days of experiment. Growth and photosynthetic activity of flax reduced to an average of 21 and 45 %, respectively. However, inoculation of G. intraradices significantly increased the plant biomass even under metal stressed conditions. Additionally, mycorrhizal association also assists the Cd and Cr increased uptake by 23 and 33 %, respectively. Due to metal stress, chlorophyll contents were decreased by 27 and 45 %, while 84 and 71 % increased proline content was observed under Cd and Cr stress, respectively. The present results clearly signify the differential response and potential of flax plant towards heavy metal tolerance and accumulation that can further increase with mycorrhizal fungus.