Detection of microplastics and phthalic acid esters in sea urchins from Sardinia (Western Mediterranean Sea).

The occurrence of microplastics (MPs) and phthalic acid esters (PAEs) in wild purple sea urchins (Paracentrotus lividus) of Sardinia (Italy, Western Mediterranean Sea) was surveyed. Specifically, MPs were analyzed in the digestive tract by µFTIR and PAEs in the gonads by SPME-LC-MS/MS. 9 out of 22 specimens resulted contaminated with MPs and 20 displayed levels of PAEs over the quantification limit. A total of 23 MPs were detected with a maximum concentration of 4 microplastics/individual in the commercially undersized specimens. PAEs displayed average concentration of 32 ng/g, σ = 5.3 with maximum value of 77 ng/g. The most abundant congeners were DEHP (17 ng/g, σ = 4.3) and DBP (10 ng/g, σ = 2.5). Statistical analysis showed correlation between DEHP and fiber concentrations and among the concentration of MEP, DEP, DBP and BBzP. Due to local use of sea urchin gonads as gourmet delicacy, the potential human exposition to MPs and PAEs by consumption is also discussed.

Microplastics in the first-year sea ice of the Novik Bay, Sea of Japan.

Sea ice is heavily contaminated with microplastics particles (MPs, <5 mm). First-year sea ice cores (38-41 cm thick) were taken in the beginning of spring in a narrow populated bay of the Sea of Japan. Two ice cores were examined (layer-by-layer, excluding surface) for MPs content: one using µ-FTIR for 25-300 µm (SMPs), and another one - with visual+Raman identification for 300-5000 µm particles (LMPs). The integral (25-5000 µm) bulk mean abundance of MPs was found to be 428 items/L of meltwater, with fibers making 19 % in SMPs size range and 59 % in LMPs. Integral mean mass of MPs was estimated in 34.6 mg/L, with 99.6 % contribution from fragments of LMPs. Comparison with simple fragmentation models confirms deficit of SMPs (especially of fibers in size range 150-300 µm), suggested to result from their leakage with brine. Multivariate statistical analysis indicates strong positive correlation of large fiber (>300 µm) counts and ice salinity.

First detection of microplastics in reef-building corals from a Maldivian atoll.

The presence of microplastics in the world's oceans and their effects on marine habitats are highly concerning. As suspension-feeders, corals are very exposed to microplastics, compromising the health of coral reef ecosystems. In this study we surveyed for the first time the presence of microplastics in Maldivian reef-building corals. Aiming to determine the influence of exposure and depth on microplastic distribution, analyses were carried out on 38 individuals belonging to three different species. 58% of the investigated colonies resulted contaminated with particles within the 25-150 µm size range. The maximum concentration was encountered in a Pocillopora verrucosa colony sampled from a shallow inner reef (8.9 particles/g of coral). No significant differences in microplastic concentration were observed between different depths, exposures, sites and species. Overall, this study confirmed microplastic presence in coral reefs of the Maldivian archipelago including foundation species.

Phthalates bioconcentration in the soft corals: Inter- and intra- species differences and ecological aspects.

The bioconcentration of dimethyl phthalate (DMP) diethyl phthalate (DEP) dibutyl phthalate (DBP) butyl benzyl phthalate (BBzP), di-(2-ethy hexyl) phthalates (DEHP), mono-butyl phthalate (MBP), mono-benzyl phthalate (MBzP), mono-(2-ethy hexyl) phthalate (MEHP) in the soft corals Coelogorgia palmosa, Sinularia sp., Sarcophyton glaucum, and Lobophytum sp. was investigated. Specimens were cultured in a microcosm environment built-up at the Genova Aquarium and analyses were carried out by in vivo SPME-LC-MS/MS. The distributions of the phthalates among the four surveyed species resulted significantly different. Calculated bioconcentration factors (BCFs) showed values spanning over two orders of magnitude, from a minimum of log10 BCFDEP = 1.0 in Sarcophyton glaucum to a maximum of log10 BCFDBP = 3,9 calculated for Coelogorgia palmosa. Moreover, the calculated BCFs of the long chain phthalates resulted up to three orders of magnitude lower than theoretically predicted (from logKow), whereas BCF of short chain phthalates resulted higher. This, together with the detection of phthalic acid monoesters, suggests the presence of species-specific different metabolic transformation among the surveyed soft coral species that involve DEHP.

Detection of plastic particles in marine sponges by a combined infrared micro-spectroscopy and pyrolysis-gas chromatography-mass spectrometry approach.

Plastic pollution threatens the marine environment, especially due to the adverse effects caused by micro and nano particles interacting with the marine biota. In order to provide reliable data regarding micro and nanoplastic contamination and the related impacts, efficient analytical solutions are needed. We developed a new analysis workflow that uses marine sponges to monitor plastic pollution by characterizing the plastic particles accumulated in their tissue. Specimens of cf. Haliclona (Haplosclerida) were sampled in the Maldivian archipelago. The aim was to optimize the method and to carry out a pilot study of the contamination of the related reef habitat. Particles were isolated, size fractioned, counted and submitted to morphological and chemical characterization. The constituting polymer was identified by infrared microspectroscopy for particles >25 µm, and by pyrolysis coupled with gas chromatography mass spectrometry for those <25 µm. Method recoveries were between 87 and 83% and limits of quantitation (LOQs) were between 6.6 and 30.2 ng/g. Analyses showed that 70% of the sponges presented plastic contamination, with an average of 1.2 particles/g tissue for the 25-150 µm size range, and a total plastic concentration of up to 4.8 µg/g in the 0.2-25 µm size range, with polyolefin being the most represented polymer in both size ranges. Overall, the study demonstrated the reliability of the proposed analytical workflow and of the use of sponges as biosamplers for plastic particles.

Extraction of microplastic from marine sediments: A comparison between pressurized solvent extraction and density separation.

Due to the ecotoxicological effects, microplastics are considered a threat for the marine environment. Recent reports indicate their presence not only in subsurface water and in coastal beach sediments, but also in the deep-sea. Notwithstanding, there is still not a scientific consensus about the analytical procedure to be applied for their determination. In this work we compared the performance of two extraction methods: pressurized solvent extraction (PSE) and density separation. Sea sand and seafloor sediments were spiked with known amounts of polystyrene (PS), polyethylene (PE) and polypropylene (PP) microplastics and submitted to both the extraction procedures. Results showed that the PSE ensured higher recoveries for the smaller size particle fractions (89,2 ± 1.1% in the 50-150 µm range) whereas the density separation enabled precise recoveries for the larger size particles (SD = 1,5%). No significant differences in terms of blanks control were highlighted.