Elastic Textile Wristband for Bioimpedance Measurements.

In this paper, wristband electrodes for hand-to-hand bioimpedance measurements are investigated. The proposed electrodes consist of a stretchable conductive knitted fabric. Different implementations have been developed and compared with Ag/AgCl commercial electrodes. Hand-to-hand measurements at 50 kHz on forty healthy subjects have been carried out and the Passing-Bablok regression method has been exploited to compare the proposed textile electrodes with commercial ones. It is demonstrated that the proposed designs guarantee reliable measurements and easy and comfortable use, thus representing an excellent solution for the development of a wearable bioimpedance measurement system.

Molecular cloning of silicatein gene from marine sponge Petrosia ficiformis (Porifera, Demospongiae) and development of primmorphs as a model for biosilicification studies.

In some sponges peculiar proteins called silicateins catalyze silica polymerization in ordered structures, and their study is of high interest for possible biotechnological applications in the nanostructure industry. In this work we describe the isolation and the molecular characterization of silicatein from spicules of Petrosia ficiformis, a common Mediterranean sponge, and the development of a cellular model (primmorphs) suitable for in vitro studies of silicatein gene regulation. The spicule of P. ficiformis contains an axial filament composed of 2 insoluble proteins, of 30 and 23 kDa. The 23-kDa protein was characterized, and the full-length cDNA was cloned. The putative amino acid sequence has high homology with previously described silicateins from other sponge species and also is very similar to cathepsins, a cystein protease family. Finally, P. ficiformis primmorphs express the silicatein gene, suggesting that they should be a good model for biosilicification studies.