RESUMO
A survey was conducted from 1999 to 2003 as part of a microbial biodiversity study on geophilic and keratinophilic fungi in central India. Among the keratinophilic fungi recovered were 82 isolates belonging to the dermatophyte genus Microsporum. Species were provisionally identified by morphology and confirmed by PCR-RFLP and sequencing of the ITS regions of rDNA. Microsporum persicolor appeared to be preponderant in central Indian soils, outnumbering the common geophilic species of Microsporum filvum and Microsporum gypseum. Three dinucleotide microsatellite markers were developed and their use revealed immense intraspecific variation among Indian populations of M. persicolor which would indicate that this species was not recently introduced into India. No correlation was established between the genotypes and the geographical location or the habitat of the isolates.
Assuntos
Microsporum/genética , Microsporum/isolamento & purificação , Microbiologia do Solo , DNA Fúngico/genética , DNA Ribossômico/genética , DNA Espaçador Ribossômico/genética , Ecossistema , Índia , Repetições de Microssatélites , Microsporum/classificação , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNARESUMO
Recently a new taxon of geophilic dermatophytes was established as Microsporum appendiculatum Bhat and Mariam, based on the presence of appendaged macroconidia. However, such appendages are already known in the related species Microsporum gypseum. We conducted a survey of soil in central India as a part of a microbial biodiversity project and obtained two strains of M. gypseum with appendaged macroconidia. Using phenotypical characterization in combination with sequencing and restriction fragment length polymorphism (RFLP) of the Internal Transcribed Spacer (ITS) region of rDNA, we found that all strains of appendaged species are identical. Therefore M. appendiculatum is regarded as a synonym of M. gypseum.
