Evaluation of work place pesticide concentration and health complaints among women workers in tea plantation, Southern India.

BACKGROUND: Women contribute fifty percent of the total work force in tea plantation sector. The continuous use of chemical pesticides in plantation may increase the risk of exposure and can cause detrimental health effects. OBJECTIVES: To assess the health status of women worker exposed to pesticides in tea plantation and to evaluate the pesticide levels in work environment. METHODS: A cross sectional study was performed (n = 204) to assess the health complaints among exposed women workers (n = 116) and non-exposed women (n = 88) using a predesigned questionnaire. Work environmental air samples collected using PUF sampler and personal samplers were analyzed for various pesticides. RESULTS: Musculoskeletal disorder, skin color changes, allergies and asthma were the most commonly reported health complaints. Notably, only 16% of the women used personal protective equipment (PPE) while 68% reported to have experienced tea plantation work up to 6 month of their pregnancy period. Chlorpyrifos methyl (8.3%), carbendazim (6.7%), thiamethoxam (4.2%), ethion (4.2%) and thiophanate methyl (2.1%) were detected in work place air samples. SIGNIFICANCE: This study is the first to characterize pesticide levels in work place of women workers in India. Women are continuously exposed to pesticides through their work and environmental exposure. Preventive measures are essential to mitigate the adverse health effects of pesticides among women workers.

Assessment of genotoxicity and cholinesterase activity among women workers occupationally exposed to pesticides in tea garden.

To assess the effects of exposure to pesticides in tea garden women workers, the present study evaluated the activities of erythrocyte acetylcholinesterase (AChE) and plasma butyrylcholinesterase (BChE) as biomarkers of pesticide exposure and DNA damage by comet assay as a biomarker of genotoxicity in peripheral blood lymphocytes. We examined 143 women subjects including 77 women workers exposed to pesticide in tea garden (exposed) and 66 women subject with no history of occupational exposure to pesticides (non-exposed). The two groups had similar mean ages, BMI and personal habits. The results revealed that exposed group had lower activity of AChE (2.86 ± 0.75 vs. 3.93 ± 0.87 U/ml) BChE (2.02 ± 0.74 vs. 2.60 ± 0.74 U/ml, p < 0.001) than those of non-exposed group. The mean comet parameters such as tail length (TL), % DNA in tail, tail moment (TM) and olive tail moment (OTM) used to measure DNA damage were significantly elevated in exposed workers compared to non-exposed (p < 0.001). There was no variation observed in Cholinesterase (ChEs) activity and Comet assay parameter based on age, personal habits, years of exposure and use of personal protective equipments (PPEs) except BChE activity. The multiple regression analysis showed no association of confounding factors on end points measured except slight negative association of BMI with tail length. Therefore this study suggests that the workers in tea garden might have exposed to pesticide through their occupation on a regular interval. Although this study could able to associate it with their occupational exposure, the role of other DNA damaging factors cannot be ruled out. Our study finding indicate the necessity of periodic monitoring of these biomarkers along with imparting education and training to minimise the risk.