Antimicrobial activity of probiotic bacteria-mediated cadmium oxide nanoparticles against fish pathogens.

The current research was designed to investigate the antibacterial activity of probiotic bacteria mediated cadmium oxide nanoparticles (CdO NPs) on common fish pathogenic bacteria like Serratia marcescens, Aeromonas hydrophila, Vibrio harveyi, and V. parahaemolyticus. CdO NPs were synthesized using probiotic bacteria as follows: Lactobacillus species with different precursor of cadmium sulfate concentrations (5, 10, and 20 mM). The average crystalline sizes of the CdO NPs were determined based on the XRD patterns using the Debye-Scherrer equation for different precursor concentrations. Specifically, sizes of 40, 48, and 67 nm were found at concentrations of 5, 10, and 20 mM, respectively. The antibacterial efficacy of CdO NPs was estimated using a well diffusion assay, which demonstrated the best efficacy of 20 mM CdO NPs against all pathogens. AFM analysis of nanoparticle-treated and untreated biofilms was performed to further validate the antibacterial effect. Antibacterial activity of CdO nanoparticles synthesized at varying concentrations (5, 10, and 20 mM) against fish pathogens (S. marcescens, A. hydrophila, V. harveyi, and V. parahaemolyticus). The results indicated the highest inhibitory effect of 20 mM CdO NPs across all concentrations (30, 60, and 90 µg/mL), demonstrating significant inhibition against S. marcescens. These findings will contribute to the development of novel strategies for combating aquatic diseases and advancing aquaculture health management practices.

Preparation of Herbal Nano-Formulation-Assisted Mouth Paint Using Titanium Dioxide Nanoparticles and Its Biomedical Applications.

Background Mouth paint is a liquid oral care solution applied topically to the mouth, formulated to address oral health concerns like bacterial infections, inflammation, and bad breath. To improve the effectiveness of oral healthcare, nanomaterials are utilized in many dental products. Titanium dioxide nanoparticles (TiO2NPs) exert their antimicrobial effects through mechanisms like producing reactive oxygen species and direct interaction with microbial cells. The current study explored the antimicrobial, anti-inflammatory, and cytotoxic effects of the mouth paint prepared using TiO2NPs using an aqueous formulation of lemongrass and dry ginger. Methods Mouthpaint prepared using TiO2NPs synthesized using lemongrass and ginger was prepared and tested for potential applications. The antimicrobial activity of the prepared TiO2NPs-mediated mouth paint at different concentrations (25, 50, and 100 µL) against oral pathogens (Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus, and Candida albicans) was evaluated using the Agar well diffusion method. The anti-inflammatory activity of the produced mouth paint was examined using a bovine serum albumin denaturation assay and an egg albumin denaturation assay. The cytotoxic effect of the produced mouthpaint was analyzed using a brine shrimp lethality assay.  Results Green synthesized TiO2NPs showed potent antimicrobial activity against the tested oral pathogens by exhibiting a zone of inhibition of 11 mm on Petriplate against Staphylococcus aureus and Candida albicans at 100 µL concentration. The prepared nanoparticles-mediated mouth paint possesses significant anti-inflammatory activity by effectively preventing the denaturation of bovine serum albumin, with a 74% inhibition at a concentration of 50 µL. The egg albumin denaturation assay showed a percentage inhibition of 80% at 50 µL. At the lowest concentration of 5 µL of the prepared mouth paint, 90% of the nauplii (egg-to-larvae stage of brine shrimp) remained alive after 48 hours. Conclusion The results showed that mouthpaint prepared using titanium dioxide nanoparticles synthesized using lemongrass and dry ginger formulations possessed significant antimicrobial activity and also displayed potential anti-inflammatory activity. The prepared mouth paint also displayed less toxicity, and hence, it can be used as an alternative to the commercially available synthetic mouth paint, which has more side effects.

Antibacterial, antioxidant and anti-inflammatory activity zinc-titanium dioxide nanocomposite.

The current study concentrated on the green synthesis of Zinc-titanium dioxide nano-composite (Zn-TiO2 NC) through the use of lemon extract, optimizing the different experimental factors required for the formation and stability of nanocomposite. The preparation of nanocomposite was confirmed by the observation of the colour change and the surface plasmon resonance band was found at 380 nm, utilizing UV-Visible analysis. The TEM analysis, the morphological features of the prepared nanocomposite was identified to be spherical shape with mean particle size of 25 nm. In addition, the antibacterial, antioxidant, and anti-inflammatory activity of this nano-composite were also investigated. The biosynthesized nanocomposite showed excellent antibacterial activity against S. mitis and S. mutans. The obtained results indicate that the antioxidant and anti-inflammatory activity of this nanocomposite is significant. This bioactive nanocomposite can be used as an effective antibacterial, antioxidant and anti-inflammatory agent in biomedical and pharmacological fields for future applications.

Formulation and evaluation of a novel herbal trio gel containing flax seed extract, carbopol and carboxymethyl cellulose.

It is of interest to formulate and evaluate herbal trio gel containing brown flax seed extract, carbopol, and carboxymethyl cellulose and to assess the antimicrobial, anti-inflammatory activity along with quality analysis using SEM and FTIR. The brown flax seeds were grinded into a fine powder and supercritical fluid was prepared which was mixed with CMC and carbopol. The formulation was checked for antimicrobial, anti-inflammatory activity, surface characteristics with SEM and FTIR. The results revealed that the activity of the trio gel was efficacious in hampering the growth of black pigmented anaerobes. The highest zone of inhibition for novel herbal trio gel was recorded at 100 µL measuring 14mm and for the standard chlorhexidine gel it was recorded at 100 µL measuring 23mm in diameter. The results proved that the zone of inhibition of novel herbal trio gel had a decent difference to that of standard chlorhexidine gel. The anti-inflammatory activity showed significant activity at 20 µL which accounted for 53% for the novel herbal trio gel and for the standard diclofenac gel it showed highest activity at 20 µL which accounted for 60%. However, there was not much difference between the herbal trio gel and standard diclofenac gel. SEM observations revealed that the components used in formulation of this trio gel have been bonded well to each other and exhibited appreciable surface characteristics. The lattice of the trio gel has been very well exhibited in SEM analysis. FTIR revealed high peaks showing the different components present in the trio gel. Within the limitations of the study, the results of our study concluded that novel herbal trio gel containing Linum usitatissimum extract, sorbitol, and carboxymethyl cellulose could be an efficient economic primeval substitute that is non-toxic, natural, and structured for clinical application.

Application of nanoparticles in Dentistry.

Nanoparticles(NPs) are of particle sizes lesser than 100nm and are insoluble the field which deal with the handling of these particles is coined as "Nanotechnology" as their particle size is smaller, they can penetrate easily therefore they are applied in various medical fields, drug delivery and in dentistry as they have antimicrobial property, reduces friction, anti-inflammatory and antioxidant property. Many studies have been done to evaluate its application and its cytotoxicity by varying its concentration and various studies have been done to evaluate its physical property. Therefore, it is of interest to describe concepts of nanoparticles, mode of action, tissue reaction and its application in orthodontics.

Terminalia chebula-Assisted Silver Nanoparticles: Biological Potential, Synthesis, Characterization, and Ecotoxicity.

In the current research, an aqueous extract of Terminalia chebula fruit was used to produce silver nanoparticles (Ag NPs) in a sustainable manner. UV-visible spectrophotometry, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used to characterize the synthesized nanoparticles. Synthesized Ag NPs were detected since their greatest absorption peak was seen at 460 nm. The synthesized Ag NPs were spherical and had an average size of about 50 nm, with agglomerated structures, as shown via SEM and TEM analyses. The biological activities of the synthesized Ag NPs were evaluated in terms of their antibacterial and antioxidant properties, as well as protein leakage and time-kill kinetics assays. The results suggest that the green synthesized Ag NPs possess significant antibacterial and antioxidant activities, making them a promising candidate for therapeutic applications. Furthermore, the study also evaluated the potential toxicological effects of the Ag NPs using zebrafish embryos as a model organism. The findings indicate that the synthesized Ag NPs did not induce any significant toxic effects on zebrafish embryos, further supporting their potential as therapeutic agents. In conclusion, the environmentally friendly production of Ag NPs using the extract from T. chebula is a promising strategy for discovering novel therapeutic agents with prospective uses in biomedicine.

Green Synthesis, Characterization and Bioactivity of Mangifera indica Seed-Wrapped Zinc Oxide Nanoparticles.

In the realm of nanoparticles, metal-based nanoparticles have traditionally been regarded as the pioneering category. Compared to other nanoparticles, zinc oxide nanoparticles have several advantages, including optical and biological properties, which provide them a significant competitive advantage in clinical and biological applications. In the current investigation, we used an aqueous Mangifera indica seed extract to synthesize nanoparticles of zinc oxide (ZnO NPs). UV-Vis spectroscopy, Fourier transform infrared spectroscopy analysis, atomic force spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy were used to characterize the synthesized ZnO NPs. The nanoparticles were assessed for their potential to inhibit bacterial growth and protect cells from free radical damage. According to the current study's findings, zinc oxide nanoparticles that had been modified with the aid of mango seeds were very efficient in preventing the development of the tested bacteria and were also powerful antioxidants.

Cytotoxicity, Antimicrobial, Anti-inflammatory and Antioxidant Activity of Camellia Sinensis and Citrus Mediated Copper Oxide Nanoparticle-An In vitro Study.

Aim: Several applications of copper oxide nanoparticles (CuONPs) have been documented in various fields, including healthcare, dentistry, medication delivery, tissue and cancer imaging, biolabeling, and biosensing. Therefore, this study aimed to synthesize CuONPs using the plant extracts of Camellia Sinesis (CS) and citrus limon (CL). The nanoparticles were then evaluated for their cytotoxicity, antibacterial, anti-inflammatory, and antioxidant activities. Materials and Methods: CuONPs were prepared using CS and CL through the green synthesis method. The Zone of Inhibition (ZOI) test was used to assess the antibacterial activity against strains of Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, and Candida albicans. The albumin denaturation assay was used to assess the substances' anti-inflammatory activity. The cytotoxicity was determined by conducting the brine shrimp lethality test. Additionally, the antioxidant nature was tested using the 1,1-diphenyl-2-picryl hydrazyl method. Results: CuONPs mediated by CS and CL were successfully synthesized. The nanoparticles demonstrated significant antimicrobial activity against the bacteria being studied, specifically S. aureus. The cytotoxic effect was observed to be the least when the concentrations were below 20 µL. A potent antioxidant effect, characterized by its maximum absorbance at 517 nm, was observed at a concentration of 50 µL. A significant anti-inflammatory effect was noted for all tested concentrations. Conclusion: The use of CS- and CL-mediated CuONPs demonstrates a favorable antimicrobial effect with reduced cytotoxicity, as well as improved anti-inflammatory and antioxidant effects at higher concentrations.

Mini-Implants in Dentistry - A Review.

Mini-implants were introduced as an alternative to standard implants for use in narrow alveolar ridges due to their smaller length and size. They are also used to provide anchorage in orthodontic treatments. Mini-implants offer simplified treatment procedures with a flat learning curve, low cost, and a possible flapless surgical procedure that can decrease post-surgical morbidity. Before being implemented into clinical practice, they were subjected to in vivo testing using animal models as is with all implant research. The biomechanical properties of mini-implants have earned them a high rate of acceptance in treatment among patients. In this review, we focus on mini-implants, extrapolation from mini-implant testing on animal models, and their latest use in dentistry.

Degradation of Toxic Dye and Antimicrobial and Free Radical Potential of Environmental Benign Zinc Oxide Nanoparticles.

Several industries have become major contributors to waterbody contamination due to the improper removal of dyes and effluents into water bodies. Due to their carcinogenic properties and low biodegradability, dye degradation is a considerable danger to people, animals, and the oceanic environment. As part of this study, Andrographis paniculata leaf extract was used as a reducing and stabilizing agent to synthesize zinc nanoparticles and degrade dyes such as methyl red and eosin. Zinc oxide nanoparticles (ZnONPs) showed a surface plasmon resonance peak at 430 nm in the UV spectrum. The FTIR result showed a band at 597.93 cm-1 that confirmed the formation of zinc nanoparticles. AFM results revealed spherical ZnONPs. The SEM results predicted an average particle size of 60 nm for crystalline particles. Biologically synthesized zinc nanoparticles exhibited greater antibacterial activity against Pseudomonas spp. and Proteus spp. but lesser activity against Klebsiella spp. and S. aureus. At 1000 µg/ml concentration, ZnONPs had the highest antioxidant activity of 45.34%. An ultraviolet-visible spectrophotometer measured dye degradation progress between 300 and 800 nm. For methyl red, the maximum absorption peak was measured at 415 nm, and for eosin, the maximum peak value was measured between 500 and 515 nm.

Syringic acid and silymarin concurrent administration inhibits sodium valproate-induced liver injury in rats.

Sodium valproate (SV) is a well-known anti-epileptic drug, also used to control convulsions, bipolar disorders and migraines. SV has been shown to induce liver toxicity in clinical subjects. Syringic acid (SA), a natural polyphenolic compound has potential antioxidant, anti-inflammatory and several beneficial effects. Therefore, in this study, we evaluated hepatoprotective effect of SA against SV-induced liver injury in rats. Wistar rats were treated with SV orally at a dose of 500 mg/kg, once daily, for 14 days. Another three groups of rats were administered with SV and concurrently treated with SA (40 and 80 mg/kg) and silymarin (SIL) (100 mg/kg) for 14 days. SV administration for 14 days caused significant (p < .001) elevation of liver transaminases and ALP in serum. Liver MDA level was significantly (p < .001) increased with a concomitant decrease (p < .001) in enzymic antioxidants activities in SV administered rats. SV administration also caused the upregulation of proinflammatory markers such as tumor necrosis factor α, c-Jun N-terminal kinase, nuclear factor kappa B, cyclooxygenase-2 and Interleukin 6 expressions in liver tissue. Histopathological studies also revealed the presence of inflammatory cell infiltration and hepatocellular necrosis upon SV administration. At both doses, concurrent administration of SA and SIL significantly (p < .001) inhibited the liver transaminase activities in serum, oxidative stress, and proinflammatory markers expression in liver tissue. Our current results suggest that SA can be a promising herbal drug that can inhibit SV-induced hepatotoxicity when administered together due its potential anti-inflammatory effects.

Preparation of a Novel Nanocomposite and Its Antibacterial Effectiveness against Enterococcus faecalis-An In Vitro Evaluation.

The interest in the use of green-mediated synthesis of nanoparticles (NPs) is shown to have increased due to their biocompatibility and reduction of overall production costs. The current study aimed to evaluate a novel nanocomposite (NC) prepared by using a combination of zinc oxide, silver and chitosan with lemon extract as a cross-linking agent and assessed its antimicrobial effectiveness against Enterococcus faecalis (E. faecalis). The NPs and NC were prepared individually using a modification of previously established methods. Ananalys is of the physiochemical properties of the NC was conducted using ultraviolet-visible spectroscopy (UV-Vis) (Shimadzu Corporation, Kyoto, Japan). and transmission electron microscopy (TEM) imaging(HR-TEM; JEOL Ltd., Akishima-shi, Japan. The microbial reduction with this novel NC was evaluated by measuring the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a tube assay analytic technique. A time-kill assay analysis was conducted to evaluate the kinetic potential against E. faecalis at different time intervals. The novel NC showed a homogenous nanoparticle size under TEM imaging and under UV-Vis established an absorption range of 350−420 nm making it similar to its individual counterparts. The MIC and MIB were measured at 62.5 ± 20 mg/L (p < 0.05) and 250 ± 72 mg/L (p < 0.05), respectively. A time-kill assay analysis for the NC showed 5 h was required to eradicate E. faecalis. Based on the achieved results, it was seen that the novel NC using a combination of silver, zinc oxide and chitosan showed improved antimicrobial action against E. faecalis compared with its individual components under laboratory conditions. A complete eradication of 108 log units of E. faecalis at 250 mg/L occurred after a total of 5 h. These preliminary results establish the use of lemon extract-mediated silver, zinc and chitosan-based NC had an antibacterial effectiveness against E. faecalis similar to the individual counterparts used for its production under laboratory conditions.

In vitro anti-inflammatory activity of silymarin/hydroxyapatite/chitosan nanocomposites and its cytotoxic effect using brine shrimp lethality assay.

Silymarin, a bioactive compound, is one of the most prominent drugs used in liver diseases. Chitosan and hydroxyapatite (HAP) are the major materials used employed in many biomedical processes like drug delivery, osteointegration, etc. The nanoparticles and nanocomposites are advanced materials with many biomedical applications in diagnosis and therapeutics. In this study, HAP and chitosan were used as a polymeric material, silymarin as a bioactive compound, and other nanoparticle based combinations. The prepared individual materials and nanocomposites were used for the anti-inflammatory activity and brine shrimp lethality assay. The results clearly show that the nanocomposites are good anti-inflammatory agents with lower toxicity.

The predominance of Shiga toxin-producing E. coli in the Southeast Coast of India.

In this study, we reported Shiga toxin-producing Escherichia coli (STEC) in 847 samples, including those in coastal waters, sediments, and fish samples in the Southeast Coast of India. A total of 3742 E. coli strains were identified using conventional and molecular identification methods. Of these, 1518 isolates expressed virulent genes Stx1, Stx2, and Eae; effects on these genes on toxicity were examined. Furthermore, 2224 non-STEC isolates caused hemolytic uremic syndrome and played a key role in the persistence of STEC contamination. We conclude that toxin production is not adequate to cause disease, and the pathogenic mechanism of STEC remains poorly defined. Therefore, the present study indicates the status of pollution, highlighting the need for sanitation in public health.

ß-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells.

BACKGROUND: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using ß-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. METHODS: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. RESULTS: ß-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. CONCLUSION: Our present result suggests that ß-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo.

An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis in HepG2 cells.

Microbes have long been used for the synthesis of a variety of nanoparticles. Hepatocellular carcinoma (HCC) is the primary liver cancer and it is the second leading cause of cancer-related mortality worldwide. In this study, we have synthesized Enterococcus mediated gold nanoparticles (AuNPs) and investigated their cytotoxic potential against human hepatocellular cancer cell line (HepG2). AuNPs were synthesized using Enterococcus sp. RMAA. HepG2 cells were treated with different concentrations of AuNPs for 24 hours and cytotoxicity was analyzed by MTT ((4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. AuNPs induced reactive oxygen species expression was analyzed by 2',7'-dichlorodihydrofluorescein diacetate staining. Morphological changes related to apoptosis was analyzed by annexin V/propidium iodide staining. Protein expression of proliferating cell nuclear antigen (PCNA) was done by western blotting analysis. Bacterial-mediated AuNPs caused significant cytotoxicity in HepG2 cells. AuNPs treatment also caused the significant expression of ROS and morphological damage related to apoptosis. AuNPs treatments were responsible for the dislocation of cytochrome c from mitochondria to cytosol. The protein expression of PCNA was significantly decreased upon AuNPs treatment. These findings suggest that Enterococcus-mediated AuNPs can inhibit the proliferation of HepG2 cells via intracellular ROS mediated apoptosis, decreased PCNA expressions, and it may have the potential to treat HCC.

Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens.

The green approachable of metal nanoparticles is treated to be an eco-friendly path and cost-effectiveness. In this present study, nano copper was synthesized profitably by Cissus vitiginea. The synthesized nano copper was used to evaluate the antioxidant and antibacterial activity against urinary tract infections pathogens. The resulting constructed nanoparticles were characterized by using ultraviolet spectroscopy absorbance around 370 nm. Scanning electron microscopy results showed the distribution of nanoparticles and particles sizes are found to be in the range of 5-20 nm. X-ray diffraction spectrum characteristic diffraction peaks for copper nanoparticles were observed at 2θ ranges 35.5 and 43.2° correspond to lattice planes (1 1 1) and (2 0 2), respectively. X-ray photoelectron spectroscopy shows that two distinct peaks at binding energy resulted that the chemical states of copper. The results serve the evidence that the green mediated nano copper might indeed be the potential source to treat urinary tract infections caused by E. coli, Enterococcus sp., Proteus sp. and Klebsiella sp. This fact-finding conclusion is that C. vitiginea leaf extract based green synthesis nano copper particles proved to effectively kill it or significantly inhibit activity contra to urinary tract infection pathogens and exhibit excellent antioxidant activity.

Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells.

It is of interest to study the cytotoxicity of silibinin assisted silver nanoparticles in human colorectal (HT-29) cancer cells. Silver nanoparticles were synthesized using silibinin as a reducing agent. The synthesized silibinin assisted silver nanoparticles ( SSNPs) were characterized and analyzed using a transmission electron microscope and spectrophotometer. The SSNPs synthesized in this study are spherical and their size ranges from 10 to 80 nm. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/mL) of SSNPs and cytotoxicity was evaluated. The apoptosis was using flow cytometry. p53 protein expression using western blot. SSNPs are induced a decrease in viability and increased concentration-dependent cytotoxicity in HT-29 cells. SSNPs treatment also caused apoptosis-related morphological changes. SSNPs treatments at 8 and 16 ng/ml showed a prominent apoptotic change i.e., 70.3% and 83.6% respectively, and decreased viability of HT-29 cells 20% and 11.2% respectively as compared to control cells. SSNPs treatments induced p53 expression in HT-29 cells. Data shows that SSNPs have the potential to induce apoptosis in colorectal cancer cells. This provides insights for the further evaluation of SSNPs in fighting colon cancer.

In Vivo Type 2 Diabetes and Wound-Healing Effects of Antioxidant Gold Nanoparticles Synthesized Using the Insulin Plant Chamaecostus cuspidatus in Albino Rats.

BACKGROUND: Gold nanoparticles are known for their many applications in the fields of therapeutics and diagnosis. METHODS: This article focuses mainly on the green method of synthesizing gold nanoparticles by using the leaf powder extract of the insulin plant Chamaecostus cuspidatus and on the characterization of developed plant-mediated synthesis of gold nanoparticles. Furthermore, we investigated the free-radical scavenging activity of green-synthesized gold nanoparticles. RESULTS: The free radicals were exhibited in a dose-dependent manner. The 50% inhibition of free radicals by gold nanoparticles showed that it was similar to that of the standard inhibition. Toxicity studies generally examine changes in blood serum chemistry and cell populations in tissue morphology through histologic analysis without inducing any lethal effects in the mouse model, thereby accomplishing sustained control over the progression of diabetes mellitus, which plays a leading role in vascular complications in patients. The treatment by gold nanoparticles of the mice with diabetes for a period of 21 days restored their blood glucose, glycogen and insulin levels. CONCLUSIONS: The use of gold nanoparticles as antidiabetes materials has been achieved. Further studies are required before gold nanoparticle-based drugs are more widely used.

Seed and bark extracts of Acacia catechu protects liver from acetaminophen induced hepatotoxicity by modulating oxidative stress, antioxidant enzymes and liver function enzymes in Wistar rat model.

In this study we investigated the hepatoprotective effects and possible mechanism of Acacia catechu in acetaminophen (APAP) induced hepatotoxicity using female Wistar rat model. Hepatotoxicity was induced by oral administration of acetaminophen (750 mg/kg body weight) for 24 h. The seed (400 mg/kg body weight) and bark (400 mg/kg body weight) extract's treated groups exhibited hepatoprotective effects and was compared with well-known clinical anti-dote N-acetylcysteine (NAC). When groups treated with acetaminophen, significant increase of liver weight/body weight ratio, liver function enzymes such as alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) and decrease of antioxidant enzymes such as glutathione (GSH) and superoxide dismutase (SOD) were observed. The histopathology of APAP treated groups also showed moderate degree of sinusoidal congestion, centrilobular necrosis with polymorph nuclear cells infiltration, marked vacuolations and congestion. However, pretreatment with seed or bark extract groups decreased LPO accumulation, reduced the liver function enzymes and increased antioxidant defense enzymes. Moreover, histopathology of seed extract treated groups showed normal architecture whereas bark extract treated groups exhibited mild degree of vacuolations in the hepatocytes with minimal sinusoidal congestion. Taken together, our study concludes that A. catechu seed extract to be a more promising agent for protecting liver from APAP induced hepatotoxicity.