RESUMO
In this work, the biocompatibility and antibacterial activities of novel SnO2 nanowire coatings prepared by electron-beam (E-Beam) evaporation process at low temperatures were studied. The nanowire coatings were characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and X-ray diffraction (XRD) methods. The results of in vitro cytotoxicity and cell proliferation assays suggested that the SnO2 nanowire coatings were nontoxic and promoted the proliferation of C2C12 and L929 cells (> 90% viability). Cellular activities, cell adhesion, and lactate dehydrogenase activities were consistent with the superior biocompatibility of the nanowire materials. Notably, the nanowire coating showed potent antibacterial activity against six different bacterial strains. The antibacterial activity of the SnO2 material was attributed to the photocatalytic nature of SnO2. The antibacterial activity and biocompatibility of the newly developed SnO2 nanowire coatings may enable their use as coating materials for biomedical implants.
Assuntos
Antibacterianos/síntese química , Materiais Biocompatíveis/síntese química , Nanotecnologia/métodos , Nanofios/química , Compostos de Estanho/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Apoptose/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Lasers , Teste de Materiais , Camundongos , Testes de Sensibilidade Microbiana , Propriedades de Superfície , Compostos de Estanho/síntese química , Compostos de Estanho/farmacologia , Titânio/química , Volatilização , Difração de Raios XRESUMO
Enrofloxacin is a fluoroquinolone derivative used for treating urinary tract, respiratory and skin infections in animals. However, low solubility and low bioavailability prevented it from using on humans. Polyvinylpyrrolidone (PVP) is an inert, non toxic polymer with excellent hydrophilic properties, besides it can enhance bioavailability by forming drug polymer conjugates. With the aim of increasing solubility and bioavailability, enrofloxacin thin films were prepared using PVP as a polymer matrix. The obtained oral thin films exhibited excellent uniformity and mechanical properties. Swelling properties of the oral thin films revealed that the water uptake was enhanced by 21%. The surface pH has been found to be 6.8±0.1 indicating that these films will not cause any irritation to oral mucosa. FTIR data of the oral thin films indicated physical interaction between drug and polymer. SEM analysis revealed uniform distribution of drug in polymer matrix. In vitro drug release profiles showed enhanced release profiles (which are also pH dependant) for thin films compared to pure drug. Antibacterial activity was found to be dose dependent and maximum susceptibility was found on Klebsiella pneumonia making this preparation more suitable for respiratory infections.
Assuntos
Antibacterianos/administração & dosagem , Portadores de Fármacos/química , Fluoroquinolonas/administração & dosagem , Povidona/química , Administração Oral , Antibacterianos/química , Antibacterianos/farmacologia , Varredura Diferencial de Calorimetria , Relação Dose-Resposta a Droga , Liberação Controlada de Fármacos , Enrofloxacina , Fluoroquinolonas/química , Fluoroquinolonas/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Microscopia Eletrônica de Varredura , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de SuperfícieRESUMO
The ethanolic extracts of the dry fruits of Caesalpinia pulcherrima, aerial parts of Euphorbia hirta and flowers of Asystasia gangeticum were tested for antimicrobial activity. The three plants exhibited a broad spectrum of antimicrobial activity, particularly against Escherichia coli (enteropathogen), Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus.
Assuntos
Acanthaceae/química , Antibacterianos/análise , Antifúngicos/análise , Caesalpinia/química , Euphorbia/química , Testes de Sensibilidade MicrobianaRESUMO
The ethanolic extracts of the leaves and flowers of Cleome viscosa and roots of Gmelina asiatica were tested for antimicrobial activity. The two plants exhibited a broad spectrum of antimicrobial activity, particularly significative against Escherichia coli , Proteus vulgaris and Pseudomonas aeruginosa. The leaf extract of C. viscosa showed moderate activity against pathogenic fungi.
Assuntos
Anti-Infecciosos/farmacologia , Cleome/química , Lamiaceae/química , Extratos Vegetais/farmacologia , Aspergillus niger/efeitos dos fármacos , Bacillus subtilis/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Enterococcus faecalis/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Flores/química , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Raízes de Plantas/química , Plantas Medicinais , Proteus vulgaris/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Rhizopus/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
The liquid membrane phenomenon in the actions of digitalis glycosides (digitoxin, digoxin and ouabain) has been studied. Formation of liquid membranes, in series with a supporting membrane, by digitalis alone and by digitalis in association with lecithin and cholesterol has been demonstrated. The results obtained on the transport of relevant permeants, viz. sodium, potassium and calcium ions and dopamine, adrenaline, noradrenaline and serotonin, in the presence of the liquid membrane generated by digitalis in association with lecithin and cholesterol indicate that the liquid membrane barrier to transport may have a relevance with the biological actions of digitalis.
Assuntos
Digitoxina/química , Digoxina/química , Ouabaína/química , Colesterol/química , Digitoxina/farmacologia , Digoxina/farmacologia , Membranas/metabolismo , Micelas , Ouabaína/farmacologia , Permeabilidade , Fosfatidilcolinas/química , Potássio/metabolismo , Sódio/metabolismo , Propriedades de SuperfícieRESUMO
Modifications in the transport of relevant permeants to their respective sites of action due to the liquid membranes formed by prostaglandins in association with lecithin and cholesterol have been discussed in the light of biological actions of prostaglandins. It has been shown that the phenomenon of liquid membrane formation is likely to make a significant contribution to the biological actions.
Assuntos
Alprostadil/fisiologia , Dinoprosta/fisiologia , Fluidez de Membrana/fisiologia , Membranas Artificiais , PermeabilidadeRESUMO
The liquid membrane phenomenon in benzodiazepines has been studied. Transport of glycine, GA-BA, noradrenaline, dopamine and serotonin in the presence of the liquid membranes generated by the benzodiazepines in association with lecithin and cholesterol has been studied. The data indicate that modification in permeabilities in the presence of the liquid membranes is likely to make a significant contribution to several biological actions of the benzodiazepines.