RESUMO
The results on in vitro culture establishment, plantlet regeneration and rooting of Camelina sativa cultivar sample Peremozhets and cultivar Mirazh are presented. Effective concentrations of sterilizing agents and duration of plant material treatment were estimated. Phytohormone ratio, sucrose concentration in nutrient medium that induce effective formation of C. sativa shoots and NAA concentration for plantlet rooting have been established. The method of Agrobacterium-mediated transformation of Camelina by using binary vector pGH217 carrying reporter beta-glucoronidase (gus) gene driven under 35S CaMV promoter and nos-terminator, and selective marker hpt gene conferring hygromycin-resistance in transgenic plant was elaborated.
Assuntos
Brassicaceae/crescimento & desenvolvimento , Brotos de Planta/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Regeneração/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Transformação Genética/efeitos dos fármacos , Agrobacterium/genética , Compostos de Benzil/farmacologia , Brassicaceae/genética , Brassicaceae/fisiologia , Cinamatos/farmacologia , Técnicas de Cocultura , Glucuronidase/genética , Higromicina B/análogos & derivados , Higromicina B/farmacologia , Ácidos Naftalenoacéticos/farmacologia , Reguladores de Crescimento de Plantas/farmacologia , Brotos de Planta/genética , Brotos de Planta/fisiologia , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/fisiologia , Purinas/farmacologia , Sementes/genética , Sementes/fisiologiaRESUMO
References are analyzed concerning production and studying of transgenic plants tolerant to various kinds of abiotic stresses; mechanisms of plant protection against stresses; the genes encoding the great spectrum of compounds giving to plants ability to survive in conditions of the stresses considerably repressing development and even lethal for control plants.
Assuntos
Adaptação Fisiológica/genética , Plantas Geneticamente Modificadas , Estresse Fisiológico/genética , Transgenes , Fenômenos Fisiológicos Vegetais/genética , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/enzimologia , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/fisiologiaRESUMO
Data on selection and characterizing of finger millet Eleusine coracana (L.) Gaertn. somaclonal variants regenerated from callus culture are presented and described. Among the tested genetically stable lines the somaclonal variant SE-7 was the most interesting due to its acquired important agricultural traits, such as a higher seed and biomass yield, rapid seed germination at lower temperatures, shortening of the main plant development stages. Data analysis shows that somaclonal variability can be a source to obtain initial material for further selection of new finger millet varieties.
