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1.
Malays Orthop J ; 16(1): 91-96, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35519523

RESUMO

Introduction: Lateral epicondylitis is a common condition causing severe incapacitating pain. Several methods of treatment have been approached for its management. In our study we aim to compare the results of injecting steroid and lignocaine mixture via single injection and peppered injection technique and analyse the outcome in each category. Materials and methods: A prospective randomised study comprising of 25 patients in each group (single vs peppered group) were included in the study after satisfying inclusion and exclusion criteria. Outcome of the treatment was measured in the form of Patient Related Tennis Elbow Evaluation (PRTEE) Questionnaire, Visual analogue score (VAS) and tenderness grading at two weeks, six weeks and six months after injection. Results: Results of our study showed that the mean PRTEE score was 22.36, 18.40 and 14.16 at 2 weeks, 6 weeks and 6 months following peppered injection as compared to 28.96, 21.84 and 25.32 in the single injection group (p value <0.05). VAS score at 2 weeks, 6 weeks and 6 months after the peppered injection was found to be 2.72, 1.72 and 1.36 and in the single injection group was 2.96, 1.92 and 2.72 at 2weeks, 6 weeks and 6 months, respectively (p value <0.05). On comparison of the 2 groups, there was a significant reduction of VAS scores at 6 months post-injection (p value <0.05) and PRTEE score at 6 weeks, 6 months in peppered injection group. Conclusion: The effects of peppered injection technique is seen to be advantageous over the single injection technique in the management of chronic lateral epicondylitis.

2.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-934980

RESUMO

@#Introduction: Lateral epicondylitis is a common condition causing severe incapacitating pain. Several methods of treatment have been approached for its management. In our study we aim to compare the results of injecting steroid and lignocaine mixture via single injection and peppered injection technique and analyse the outcome in each category. Materials and methods: A prospective randomised study comprising of 25 patients in each group (single vs peppered group) were included in the study after satisfying inclusion and exclusion criteria. Outcome of the treatment was measured in the form of Patient Related Tennis Elbow Evaluation (PRTEE) Questionnaire, Visual analogue score (VAS) and tenderness grading at two weeks, six weeks and six months after injection. Results: Results of our study showed that the mean PRTEE score was 22.36, 18.40 and 14.16 at 2 weeks, 6 weeks and 6 months following peppered injection as compared to 28.96, 21.84 and 25.32 in the single injection group (p value <0.05). VAS score at 2 weeks, 6 weeks and 6 months after the peppered injection was found to be 2.72, 1.72 and 1.36 and in the single injection group was 2.96, 1.92 and 2.72 at 2weeks, 6 weeks and 6 months, respectively (p value <0.05). On comparison of the 2 groups, there was a significant reduction of VAS scores at 6 months post-injection (p value <0.05) and PRTEE score at 6 weeks, 6 months in peppered injection group. Conclusion: The effects of peppered injection technique is seen to be advantageous over the single injection technique in the management of chronic lateral epicondylitis.

3.
Sci Rep ; 11(1): 23084, 2021 11 29.
Artigo em Inglês | MEDLINE | ID: mdl-34845252

RESUMO

Dynamic traffic of multicast communication in the Software Defined Network environment focused less though it is more natural and practical. In multicast communication, the traffic is dynamic due to the dynamic group memberships (i.e., participants join and leave the group anytime), which are not explored much in the previous research works. The multicast in dynamic traffic requires a method to handle dynamic group membership and minimum tree alteration for every join and leave of participants from the multicast group. This paper proposes a multicast tree construction algorithm, which considers receiving devices and network capability as base parameters to construct the multicast path. The proposed routing method uses Dijkstra's Shortest Path algorithm for initial tree formation, identifies a multicast path, and processes the Shortest Path Tree to reduce the overall hop count and path cost. The multicast tree generated by the proposed enables the dynamic join and leaves of participating devices with reduced tree alteration using more common paths to reach the devices. The implementation and results show that the proposed method works efficiently in resource utilization with a reduced hop count and quality for multicast communication in static and dynamic scenarios. Also, the results demonstrate that the proposed method generates a stable common path for multicast communication.

4.
Indian J Pathol Microbiol ; 63(4): 538-543, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33154302

RESUMO

BACKGROUND AND OBJECTIVES: Cisplatin is one of the major drugs that used in the treatment of oral cancer.Excision repair cross-complementation group 1 (ERCC1) is a key DNA repair gene in the nucleotide excision repair pathway which is activated in the repair of intra- and interstrand DNA crosslink caused by platinum-based treatment. The aim of this study was to investigate the association between polymorphisms in ERCC1 (C118T & C8092A) genes and the response to cisplatin-based chemotherapy. METHODS: ERCC1polymorphisms (C118T & C8092A) were studied using PCR-RFLP method from 150 OSCC patients as cases as well as 150 normal tissues from the same patients were collected as controls for this study. Results: Frequencies of ERCC1 C118C, C118T and T118T genotypes were 60%, 28% and 12% in OSCC patients and 78%, 19% and 3% in the controls, respectively. The C118T & T118T genotype had a 1.69 and 4.97 -folds increased risk for OSCC. Frequencies of ERCC1 C8092C, C8092A and A8092A were 78%, 18% and 4% in the OSCC patients and 89%, 10%, amd 1% in the controls, respectively. The C8092A genotype showed a 1.97-fold increased risk for OSCC. INTERPRETATION & CONCLUSIONS: In conclusion, this study highlights the DNA repair gene polymorphisms that might play a role in mediating susceptibility to oral squamous cell carcinoma and cisplatin therapy. Our data suggest that the ERCC1 C118T, T118T and ERCC1 C8092A genotypes are genetic risk factors for Oral squamous cell carcinoma and ERCC1 118 C/T and C8092A polymorphisms have significant influence on clinical outcome.


Assuntos
Antineoplásicos/uso terapêutico , Carcinoma de Células Escamosas/tratamento farmacológico , Cisplatino/uso terapêutico , Proteínas de Ligação a DNA/genética , Endonucleases/genética , Neoplasias Bucais/tratamento farmacológico , Polimorfismo Genético , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/genética , Intervalo Livre de Doença , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/genética
5.
Bioorg Med Chem Lett ; 30(4): 126928, 2020 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-31889664

RESUMO

One of the most commonly used strategies to reduce hERG (human ether-a-go-go) activity in the drug candidates is introduction of a carboxylic acid group. During the optimization of PPARδ modulators, some of the compounds containing a carboxylic acid were found to inhibit the hERG channel in a patch clamp assay. By modifying the basicity of the imidazole core, potent and selective PPARδ modulators that do not inhibit hERG channel were identified. Some of the modulators have excellent pharmacokinetic profiles in mice.


Assuntos
Canais de Potássio Éter-A-Go-Go/antagonistas & inibidores , PPAR delta/química , Bloqueadores dos Canais de Potássio/química , Desenho de Fármacos , Canais de Potássio Éter-A-Go-Go/metabolismo , Meia-Vida , Humanos , Cinética , PPAR delta/genética , PPAR delta/metabolismo , Bloqueadores dos Canais de Potássio/metabolismo , Bloqueadores dos Canais de Potássio/farmacologia , Relação Estrutura-Atividade , Ativação Transcricional/efeitos dos fármacos
6.
Glob Med Genet ; 7(4): 121-127, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33693445

RESUMO

Background DNA repair systems play an important role in maintaining the integrity of the human genome. Deficiency in the repair capacity due to either mutations or inherited polymorphisms in DNA repair genes may contribute to variations in the DNA repair capacity and subsequently susceptibility to cancer. Objectives This study aimed to investigate the association between Excision repair cross-complementation groups 2 (ERCC2) single nucleotide polymorphisms (SNPs rs1799793 and rs13181) and the response to platinum-based chemotherapy among patients with oral squamous cell carcinoma (OSCC). Methodology Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to determine the polymorphism from a total of 150 OSCC patients and 150 normal tissues of same patients were collected as controls for this study. Results ERCC2 GA (Asp312Asn) AC (Lys751Gln) genotypes were significantly associated ( p = 0.0001 and p = 0.0004, respectively) with OSCC patients, when compared with the controls. These findings suggest that potentially functional SNPs in ERCC2 may contribute to OSCC risk. This study highlights the genetic variant that might play a role in mediating susceptibility to OSCC in this population. An understanding of DNA repair gene polymorphisms might not only enable risk assessment, but also response to therapy, which target the DNA repair pathway.

7.
Int J Med Mushrooms ; 19(5): 457-465, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28845774

RESUMO

Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.


Assuntos
Ganoderma/química , Polissacarídeos/análise , Esteróis/análise , Triterpenos/análise , Cromatografia Líquida de Alta Pressão , Índia , Medicina Tradicional , Polissacarídeos/isolamento & purificação , Esteróis/isolamento & purificação , Triterpenos/isolamento & purificação
8.
J Chromatogr Sci ; 54(8): 1397-407, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27226462

RESUMO

A novel, simple, sensitive and stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the quantitative determination of the geometric isomer (Trans) and other related substances in the active pharmaceutical ingredient (API) of Tafluprost (TFL), with their determination by an assay. A chromatographic separation of TFL and its impurities was achieved with a C18 analytical column, using gradient elution with mobile phase A consisting of a mixture of water, methanol and orthophosphoric acid (900:100:1, v/v) and mobile phase B consisting of a mixture of acetonitrile and water (900:100, v/v). The instrumental settings included a flow rate of 1.0 mL/min for related substances and 1.2 mL/min for the assay, a column temperature of 50°C and a detector wavelength of 210 nm, using a photodiode array detector. TFL was exposed to thermal, photolytic, hydrolytic and oxidative stress conditions and the stressed samples were analyzed by the proposed method. Peak homogeneity data of TFL were obtained by using a photodiode array detector in the stressed sample chromatograms, which demonstrated the specificity of the method for estimation in the presence of degradants. The developed method was validated for parameters such as precision, accuracy, linearity, limit of detection, limit of quantification, ruggedness and robustness as per ICH guidelines.


Assuntos
Química Farmacêutica/métodos , Cromatografia Líquida de Alta Pressão , Prostaglandinas F/normas , Espectrometria de Massas em Tandem , Química Farmacêutica/normas , Cromatografia Líquida , Estabilidade de Medicamentos , Prostaglandinas F/análise , Prostaglandinas F/metabolismo , Reprodutibilidade dos Testes
9.
Curr Pharm Biotechnol ; 17(6): 540-8, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26813302

RESUMO

Present exploration deals with the therapeutic perspective of methyl gallate isolated from the leaf extract of Acacia nilotica (L.) Delile in contrast to food-borne bacterial pathogen's viz., Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus aureus with their evolutionary succession. The extract was subjected to phytochemical analysis and isolated compound was identified as methyl gallate using UV-vis, IR and NMR spectra. It was found most potent against K. pneumoniae with its minimum inhibition concentration (MIC) of 0.32 mg/ml and minimum bactericidal concentration (MBC) at 0.62 mg/ml. The correlation of MIC values with an evolutionary succession assists the relationship between their genetic and toxic properties. The cytotoxic pursuit of methyl gallate was additionally assessed over NIH3T3 mouse fibroblast by Neutral red (NR) uptake, MTT cell proliferation assay and did not disclose any relevant influence on cell viability as well as cell proliferation. As such, the methyl gallate extracted from the leaf of A. nilotica holds massive antibacterial aptitude and hands out towards a new paradigm for food and pharmaceutical industries.


Assuntos
Acacia , Antibacterianos/farmacologia , Ácido Gálico/análogos & derivados , Animais , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Sobrevivência Celular/efeitos dos fármacos , Ácido Gálico/farmacologia , Camundongos , Testes de Sensibilidade Microbiana , Células NIH 3T3 , Filogenia , Extratos Vegetais , Folhas de Planta
10.
Tumour Biol ; 37(6): 7625-32, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26687648

RESUMO

Phosphatase and tensin homolog (PTEN) and p16INK4a (p16) genes are tumor suppressor genes, associated with epigenetic alterations. PTEN and p16 promoter hypermethylation is a major epigenetic silencing mechanism leading to cancer. The cooperation between PTEN and p16 in pathogenesis of cancers suggest that their combination might be considered as potential molecular marker for specific subgroups of patients. Hence, the present study aimed to investigate whether PTEN and p16 promoter methylations were involved in oral squamous cell carcinoma (OSCC) in south Indian subjects. DNA methylation quantitative analyses of the two candidate tumor suppressor genes PTEN and p16 were performed by methylation-specific polymerase chain reaction (MSP). Fifty OSCC biopsy samples and their corresponding non-malignant portions as controls were studied comparatively. The methylation status was correlated with the clinical manifestations. Twelve out of 50 patients (24 %) were found to be methylated for PTEN gene, whereas methylation of the p16 gene occurred in 19 out of 50 cases (38 %). A statistically significant result was obtained (P = <0.0001 and 0.017) for both PTEN and p16 genes. PTEN and p16 promoter methylation may be the main mechanism leading to the low expression of PTEN and p16 genes indicating the progress of tumor development. Our data suggest that a low PTEN and p16 expression due to methylation may contribute to the cancer progression and could be useful for prognosis of OSCC. Therefore, analysis of promoter methylation in such genes may provide a biomarker valuable for early detection of oral cancer.


Assuntos
Carcinoma de Células Escamosas/genética , Metilação de DNA , DNA de Neoplasias/genética , Regulação Neoplásica da Expressão Gênica , Genes p16 , Neoplasias Bucais/genética , PTEN Fosfo-Hidrolase/genética , Adulto , Idoso , Biomarcadores Tumorais , Biópsia , Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/patologia , Transformação Celular Neoplásica , Inibidor p16 de Quinase Dependente de Ciclina/análise , Inibidor p16 de Quinase Dependente de Ciclina/fisiologia , DNA de Neoplasias/química , Detecção Precoce de Câncer , Feminino , Humanos , Índia/epidemiologia , Masculino , Pessoa de Meia-Idade , Modelos Biológicos , Neoplasias Bucais/epidemiologia , Neoplasias Bucais/patologia , PTEN Fosfo-Hidrolase/análise , PTEN Fosfo-Hidrolase/fisiologia , Regiões Promotoras Genéticas/genética , Fatores de Risco
11.
Asian Pac J Cancer Prev ; 16(17): 7589-94, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26625766

RESUMO

BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNA molecules, implicated in several activities like initiation, progression and prognosis of various cancers. Single nucleotide polymorphisms (SNPs) in miRNA genes can lead to alteration in mRNA expression, resulting in diverse functional consequences. The aim of our study was to investigate the association of miR-149C>T and miR-196a2C>T SNPs with susceptibility to development of oral squamous cell carcinoma (OSCC) in South Indian subjects. MATERIALS AND METHODS: 100 OSCC patients and 102 healthy controls from the general population were recruited for the study. Genetic analysis was performed by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) as per a standard protocol. RESULTS: The genotype frequencies in miR-196a2 polymorphism, of TT, CT and CC in the OSCC patients were 69%,10% and 22% respectively while for control group it was 80%, 15% and 5% respectively. The CC genotype of miR196a2 polymorphism was significantly associated with oral squamous cell carcinoma. The genotype frequencies in miR-149 polymorphisms of CC, CT and TT in the oral squamous cell carcinoma (OSCC) patients were 72%, 22% and 6% respectively and for control group 88%, 12% and 0% respectively. CT and TT genotypes of miR149 polymorphism were found to be significantly associated with OSCC (p = 0.05 and 0.07). CONCLUSIONS: Our study suggests that miR-196a2C>T and miR-149C>T polymorphisms may play crucial roles in the development of OSCC in South Indian subjects.


Assuntos
Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/genética , Neoplasias de Cabeça e Pescoço/epidemiologia , Neoplasias de Cabeça e Pescoço/genética , MicroRNAs/genética , Neoplasias Bucais/epidemiologia , Neoplasias Bucais/genética , Adulto , Idoso , Feminino , Predisposição Genética para Doença , Humanos , Índia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco , Carcinoma de Células Escamosas de Cabeça e Pescoço , Adulto Jovem
12.
Med Chem ; 9(1): 77-83, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20513223

RESUMO

Siderophores are small molecules produced by bacteria under iron-scarcity conditions faced by bacteria inside host. Sideophores bind iron with high affinity (Kd < 10-25 M) and are required for iron transport into the bacterial cell. Small molecules interfering with siderophore functioning can be promising anti-mycobacterial agents. Several molecules with hydrazone as a structural feature are known to have metal chelating property. This prompted us to investigate the metal chelating ability of 2-hydrazino-pyrimidin-4(3H)-one derivatives. In this light, a library of 22 novel molecules with 2- hydrazino-pyrimidin-4(3H)-one moiety was synthesized and the compounds were evaluated against M. tuberculosis under iron-limiting and iron-rich conditions. Interestingly, several molecules showed promising (MIC: < 10 µM) selective activity under iron scarcity conditions. Furthermore, compounds were found to be nontoxic at lower concentration in VERO cell lines using MTT assay. Taken together, we have discovered novel 2-hydrazino-pyrimidin-4(3H)-one molecules active against M. tuberculosis which can be developed as potent antimycobacterial agents.


Assuntos
Antibacterianos/síntese química , Antibacterianos/farmacologia , Mycobacterium tuberculosis/efeitos dos fármacos , Sideróforos/química , Bibliotecas de Moléculas Pequenas/síntese química , Bibliotecas de Moléculas Pequenas/farmacologia , Animais , Antibacterianos/química , Chlorocebus aethiops , Humanos , Hidrazinas/síntese química , Hidrazinas/química , Hidrazinas/farmacologia , Quelantes de Ferro/química , Testes de Sensibilidade Microbiana , Estrutura Molecular , Pirimidinonas/síntese química , Pirimidinonas/química , Pirimidinonas/farmacologia , Bibliotecas de Moléculas Pequenas/química , Células Vero
13.
Ann Card Anaesth ; 13(1): 22-7, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20075531

RESUMO

Pulmonary artery thromboendarterectomy (PTE) has been regarded as a promising, potentially curative surgical procedure. However, PTE is associated with specific postoperative complications, such as reperfusion pulmonary edema and right heart failure leading to a considerable mortality of 7-24%. Despite its limitations PTE is a better surgical alternative to lung transplantation which carries high morbidity and mortality. The aim of the study is to analyze the efficacy, safety, morbidity and survival associated in the postoperative period and quality of life after six months of PTE in Indian patients. Forty-one patients with surgically correctable chronic thromboembolic pulmonary hypertension underwent pulmonary endarterectomy. All patients were in New York Heart Association (NYHA) Class II, III or IV. Preoperative mean pulmonary artery pressure was 40.98 +/- 9.29 mmHg and mean pulmonary vascular resistance was 418.39 +/- 95.88 dynes/sec/cm(-5). All patients were followed up to six months and a telephonic survey was conducted using a standard questionnaire. They were assessed and classified as per NYHA grading. There was a significant reduction in the mean pulmonary artery pressure (from 40.98 +/- 9.29 mmHg to 24.13 +/- 7.36 mmHg, P < 0.001) and pulmonary vascular resistance (from 418.39 +/- 95.88 dynes/sec/cm(-5) to 142.45 +/- 36.27 dynes/sec/cm(-5), P < 0.001) with a concomitant increase in the cardiac index (from 1.99 +/- 0.20 L/min/m 2 to 3.28 +/- 0.56 L/min/m 2 , P < 0.001) during the postoperative period. The mortality rate in our study was 12.19% (five patients). Ninety per cent of the patients reported a significant improvement in the quality of life and exercise tolerance after surgery compared to the preoperative state. Pulmonary endarterectomy is an effective and potentially curative surgical treatment for patients with severe chronic thromboembolic pulmonary hypertension. The current techniques of operation make the procedure relatively safe and long-term survival, NYHA functional status and exercise capacity improve significantly.


Assuntos
Endarterectomia/efeitos adversos , Hipertensão Pulmonar/cirurgia , Complicações Pós-Operatórias/terapia , Artéria Pulmonar/cirurgia , Adulto , Endarterectomia/mortalidade , Feminino , Humanos , Hipertensão Pulmonar/psicologia , Masculino , Qualidade de Vida , Resultado do Tratamento
14.
J Environ Sci Eng ; 51(3): 199-206, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21117435

RESUMO

A bio-kinetic study was conducted on a laboratory scale to evaluate the performance of an indigenously fabricated fixed film anaerobic reactor operated in upward flow mode for the treatment of synthetic glucose medium. The feasibility of using a combination of anaerobic treatment and reverse osmosis (RO) for generation of recyclable water from the effluent formed the basis of the study. The reactor was operated under different organic loading rates (OLR) and hydraulic residence times (HRT) at a constant feed pH of 7.0. The chemical oxygen demand (COD), biochemical oxygen demand (BOD), total alkalinity, pH and total volatile acids (TVA) were monitored in the samples drawn from different sampling ports provided in the reactor. Pilot-scale experiments using a spiral-wound thin-film composite (TFC) polyamide commercial membrane were carried out to investigate the potential of RO for processing the composite effluent emanating from the anaerobic process. Separation performance was evaluated at varying feed pressure (11-41 bar) and found to improve with increasing pressure. High rejection of dissolved solids (approximately 98%), COD, BOD and almost complete removal of color were achieved with reasonable flux and water recovery.


Assuntos
Glucose/química , Anaerobiose , Osmose , Esgotos
15.
Indian J Crit Care Med ; 12(4): 158-62, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19742264

RESUMO

BACKGROUND AND AIM: It is well known that dialysis dependent renal failure increases the likelihood of poor outcome following cardiac surgery. But the results of CABG in patients with mild renal dysfunction are not clearly established. The aim of the study is to analyze the risk of preoperative mild renal dysfunction on outcome after isolated coronary surgery. MATERIALS AND METHODS: We reviewed prospectively collected data between June 2006-Nov 2006 in 488 patients who underwent isolated CABG. We separated the data into two groups. Control group having normal renal function and study group having mild renal dysfunction (serum creatinine 1.4 mg-2.2 mg%). Among 488 patients, 412 patients were in control group and 76 patients were in the study group. RESULTS: Analysis of data showed significant postoperative complications in the mild renal dysfunction group, like increased operative mortality (7.5% vs 1.6%), increased requirement of postoperative renal replacement therapy (10% vs 1.2%), increased incidence of new onset atrial fibrillation (20% vs 4.2%) and prolonged duration of ICU stay. Multivariate analysis adjusting for known risk factors confirmed preoperative mild renal dysfunction (S.creat.1.4-2.2 mg/dl) is an independent risk factor for postoperative morbidity and mortality. (Adj. OR: 4.47; 95% CI: 1.41-14.16; P=0.010). CONCLUSION: Mild renal dysfunction is an important independent predictor of outcome in terms of in-hospital mortality and morbidity in patients undergoing CABG.

16.
J Hazard Mater ; 151(2-3): 658-61, 2008 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-17683860

RESUMO

Bioslurry phase reactor was used for the degradation of pendimethalin, a pre-emergence herbicide in the contaminated soil under aerobic environment. More than 91% degradation of pendimethalin was observed for 5 days of reactor operation augmented with sewage from effluent treatment plant (ETP). The performance of the reactor was monitored regularly by measuring pH and colony forming units (CFU). The metabolites of pendimethalin formed during degradation were identified using various analytical techniques, viz., thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS/MS). Four metabolites were formed and identified as N-(1-ethylpropyl)-3,4-dicarboxy 2,6-dinitrobenzenamine-N-oxide, N-(1-ethylpropyl)-3,4-dimethoxy-2,6-dinitrobenzenamine and benezimadazole-7-carboxyaldehyde. The reactions involved were monohydrolysis of 2-methyl groups followed by dihydrolysis. Further oxidation of amine groups and hydroxylation of propyl groups produced the above said metabolites. Degradation pathway of pendimethalin has been proposed in the bioslurry phase reactor.


Assuntos
Compostos de Anilina/química , Reatores Biológicos , Herbicidas/química , Compostos de Anilina/análise , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida/métodos , Cromatografia em Camada Fina , Poluição Ambiental , Herbicidas/análise , Concentração de Íons de Hidrogênio , Espectrometria de Massas/métodos , Modelos Químicos , Esgotos , Células-Tronco , Fatores de Tempo , Eliminação de Resíduos Líquidos
17.
Bioresour Technol ; 98(8): 1561-6, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-16935494

RESUMO

Bioremediation of di-n-butyl phthalate (DnBP) in soil was studied with various concentrations in a bioslurry phase batch reactor operated in sequenting batch mode (bioaugmented with effluent treatment plant (ETP) microflora) for a total cycle period of 96h. Process performance during the reactor operation was assessed by monitoring DnBP concentration and biochemical process parameters viz., pH, dissolved oxygen (DO), colony forming units (CFU) and oxygen uptake rate (OUR), during the sequence phase operation. The degradation rate was observed to be rapid at lower substrate concentrations and found to be slow as the substrate concentration increased. The potent bacterial strain was also isolated from the slurry phase reactor. Metabolites formed during the degradation of DnBP in the slurry phase reactor were identified. Studies on the kinetics and half-life of the reaction revealed that the degradation process followed zero-order kinetic model.


Assuntos
Bactérias/metabolismo , Reatores Biológicos , Dibutilftalato/metabolismo , Poluentes do Solo/metabolismo , Resíduos/análise , Biodegradação Ambiental , Contagem de Colônia Microbiana , Meia-Vida , Cinética , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Modelos Químicos , Oxigênio/metabolismo
18.
Bioresour Technol ; 98(13): 2584-9, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17081747

RESUMO

The influence of soil-water ratio was studied on the performance of the slurry phase bioreactor operated in sequencing batch mode (anoxic-aerobic-anoxic microenvironments) during the bioremediation of soil contaminated with pendimethalin. The performance of the reactors was evaluated at different soil-water ratios (1:5-1:25; at soil loading rate (60 kg of soil/cum-day to 12 kg of soil/cum-day)) keeping the loading rate of pendimethalin constant (133.2 g/kg of soil-day) in six reactors and variable (66.6 g/kg of soil-day to 166.6 g/kg of soil-day) in other four reactors. At 1:20 soil-water ratio, the slurry phase system showed enhanced degradation of substrate (629 microg pendimethalin/g soil). The removal efficiency of pendimethalin in the reactors was dependent on the mass-transfer rates of the substrate from the soil to the aqueous phase. Soil-water ratio and substrate loading rates showed significant influence on the substrate portioning, substrate degradation efficiency and substrate desorption rate.


Assuntos
Reatores Biológicos , Recuperação e Remediação Ambiental/métodos , Herbicidas/química , Poluentes do Solo , Solo , Água
19.
J Environ Biol ; 28(4): 819-23, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18405118

RESUMO

In the present study two strains of Thiobacillus sp were isolated from aerobic sludge of distillery and dairy effluent treatment plant using standard methods of isolation and enrichment. Experiments were conducted using isolated cultures in batch bioreactor with initial sulfide concentration of 75 and 150 mg/l. The effect of initial sulfide concentration on the activity of isolated Thiobacillus sp was studied. Sulfide oxidizing capacity was also determined at different initial sulfide concentrations. The results from the study indicate the possible isolation of Thiobacillus cultures from native source and application in the full-scale reactor


Assuntos
Indústria de Laticínios , Esgotos/microbiologia , Sulfetos/metabolismo , Thiobacillus/isolamento & purificação , Resíduos Industriais , Oxirredução , Thiobacillus/metabolismo , Microbiologia da Água
20.
Int Immunopharmacol ; 6(3): 494-8, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16428085

RESUMO

Blood vessel plays a crucial role in solid tumor development. It has been suggested that blocking of angiogenesis and the action of the cytokine VEGF could be possible in cancer therapy. In a screen for naturally occurring angiogenic inhibitors, we have identified an extract from the roots of Glycyrrhiza glabra, which has potent antiangiogenic and antitumor activity. The aqueous extract inhibits the in vivo and in vitro proliferation of Ehrlich ascites tumor cells. The angioinhibitory activity of G. glabra was confirmed by its inhibition of angiogenesis in in vivo assays, peritoneal and chorioallantoic membrane assay. Reduction in the levels of the cytokine VEGF and microvessel density count in the peritoneum of mice treated with G. glabra indicated that the plant extract decreased VEGF production and the cytokine induced neovascularization. Our results suggest that the extract from the roots of G. glabra may be a potential supplemental source for cancer therapy.


Assuntos
Carcinoma de Ehrlich/irrigação sanguínea , Carcinoma de Ehrlich/tratamento farmacológico , Proliferação de Células/efeitos dos fármacos , Glycyrrhiza , Neovascularização Patológica/tratamento farmacológico , Extratos Vegetais/farmacologia , Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores , Fator A de Crescimento do Endotélio Vascular/fisiologia , Animais , Carcinoma de Ehrlich/metabolismo , Embrião de Galinha , Glycyrrhiza/química , Cinética , Camundongos , Neovascularização Patológica/metabolismo , Raízes de Plantas/química , Solventes , Células Tumorais Cultivadas
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