Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 9 de 9
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artif Cells Nanomed Biotechnol ; 45(4): 748-757, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28064507

RESUMO

The Lantana camara Linn root extract derived gold nanoparticles (Au NPs) were characterized by Ultraviolet-Visible spectroscopy, X-ray diffraction, fourier transform-infrared, high resolution transmission electron microscopy, selected area electron diffraction pattern and energy dispersive X-ray analyses. In DPPH assay, the inhibitory concentration (IC50) of Au NPs and gallic acid was 24.17 and 5.39 µg/ml, whereas, for cytotoxicity assay, the IC50 of Au NPs was 17.72 and 32.98 µg/ml on MBA-MB-231 and Vero cells, respectively. Thus, the Au NPs possess significant in vitro antioxidant and cytotoxic properties which could be considered as potential alternate for the development of anticancer drug in future.


Assuntos
Ouro/química , Ouro/farmacologia , Lantana/química , Nanopartículas Metálicas/química , Extratos Vegetais/química , Raízes de Plantas/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Ácido Gálico/química , Humanos
2.
Artif Cells Nanomed Biotechnol ; 45(5): 990-998, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27327539

RESUMO

The silver nanoparticles synthesized from Turbinaria ornata (To-AgNPs) showed spherical with crystalline nature (20-32 nm) was evaluated against fourth instar larvae of three mosquitoes. The maximum activity of To-AgNPs was recorded on Aedes aegypti followed by Anopheles stephensi and Culex quinquefasciatus with the following lethal concentration values (µg/ml): LC50 of 0.738, 1.134, and 1.494; and LC90 of 3.342, 17.982, and 22.475, respectively. The obtained respective values (µg/ml) vis-a-vis aqueous extract (To-AE) were: 2.767 and 40.577; 4.347 and 158.399, and 7.351 and 278.994. The findings revealed that To-AgNPs could form a base for the development of an eco-friendly, low-cost pesticide.


Assuntos
Anopheles , Culex , Larva/efeitos dos fármacos , Nanopartículas Metálicas , Phaeophyceae/química , Alga Marinha/química , Prata/química , Animais , Técnicas de Química Sintética , Inseticidas/síntese química , Inseticidas/química
3.
Biochimie ; 121: 13-20, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26586108

RESUMO

Host dependent expression of early HPV oncoproteins, E6 and E7 play central role in the formation of cervical carcinoma. Presently, we have shown that the cyanidin analog, idaein chloride treatment induced dose dependent apoptosis (IC50 = 2.579 µg/ml) in HPV positive - HeLa cells. Flow cytometric analysis showed arrest of cell cycle at G1 phase with an increased sub G1 cell population on 12th h of exposure. The recorded reduced expression levels of cell cycle proteins - cyclin D, cdk 4 and cdk 6 confirmed the occurrence of cell cycle arrest at G1 phase. In addition, the idaein chloride significantly inhibited the expression of E6 and E7 proteins, resulting in p53 re-expression and hence triggering of p53 dependent apoptosis. This has been further supported by the recorded variations in the expression patterns of p21/WAF, pRb and E2F regulatory proteins. In case of mitochondrial apoptotic markers, the expression of Bax was restored and Bcl-2 level got decreased at 12th h. Cleaved caspases 3 & 9 and PARP were also observed after 3 h of treatment. Interestingly, the epigenetic regulatory enzymes (DNMTs) were inhibited by idaein chloride. Thus, idaein chloride could be a potent source for developing a drug against cervical carcinoma.


Assuntos
Antocianinas/farmacologia , Proteína Supressora de Tumor p53/metabolismo , Neoplasias do Colo do Útero/metabolismo , Antocianinas/química , Apoptose/efeitos dos fármacos , Apoptose/genética , Ciclo Celular/efeitos dos fármacos , Ciclo Celular/genética , Linhagem Celular Tumoral , Ciclina D/genética , Ciclina D/metabolismo , Quinases Ciclina-Dependentes/genética , Quinases Ciclina-Dependentes/metabolismo , Feminino , Citometria de Fluxo , Humanos , Papillomaviridae/metabolismo , Proteína Supressora de Tumor p53/genética
4.
J Photochem Photobiol B ; 153: 145-52, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26409094

RESUMO

The present study pertains to the synthesis, structural elucidation, antioxidant and in vitro cytotoxic properties of silver nanoparticles (AgNPs) from marine angiosperm, Cymodocea serrulata aqueous extract (CSAE). The characterization was made through UV-Visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), zeta potential and dynamic light scanning (DLS) analyses. The UV-Vis spectrum resulted in a strong surface plasmon resonance (SPR) at 430 nm. The average crystalline size of the AgNPs was predicted through XRD peaks that indicated the 2 theta values of 37.84°, 44.06°, 64.42° and 77.74° for Bragg's refraction index. The functional groups responsible for the bio-reduction of Ag(+) into Ag(0) were focused through FTIR spectrum. The FESEM images showed that the C. serrulata mediated AgNPs (CS-AgNPs) were spherical in shape. DPPH assay revealed the higher free radical scavenging activity in CS-AgNPs, when compared to CSAE. The cytotoxicity assay on the cervical cancer (HeLa) and African green monkey kidney (Vero) cells upon treatment with CSAE: 107.7 & 124.3 µgml(-1) and CS-AgNPs: 34.5 & 61.24 µgml(-1), respectively showed good inhibition rate. These findings highlight the fact that C. serrulata could be a potential source for developing potent drugs and further studies are needed.


Assuntos
Antioxidantes/farmacologia , Proliferação de Células/efeitos dos fármacos , Magnoliopsida/química , Nanopartículas Metálicas/química , Prata/química , Animais , Antioxidantes/química , Chlorocebus aethiops , Difusão Dinâmica da Luz , Células HeLa , Humanos , Magnoliopsida/metabolismo , Microscopia Eletrônica de Varredura , Extratos Vegetais/química , Espectroscopia de Infravermelho com Transformada de Fourier , Ressonância de Plasmônio de Superfície , Células Vero , Difração de Raios X
5.
J Photochem Photobiol B ; 148: 1-8, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25854160

RESUMO

A rapid bio-reduction of chloroauric acid (HAuCl4) was achieved by Chloroxylon swietenia DC leaf extract (CSLE), which resulted in the formation of well dispersed C. swietenia gold nanoparticles (CSGNPs). The formation of GNPs was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. The characterization of synthesized CSGNPs was made through X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), high resolution transmission electron microscopy (HRTEM) equipped with energy dispersive X-ray spectroscopy (EDX) followed by size and zeta potential analyses. The GC-MS profile of C. swietenia methanolic leaf extract (CSMLE) resulted 20 phytocomponents, among those heptacosanoic acid, 25-methyl-, methyl ester (C29H58O2) attributes highest peak area. The efficiency of the synthesized CSMLE, CSGNPs and CSLE were tested against fourth instar larvae of malarial and dengue vector, which resulted more substantial upshot than with leaf extract treated. The Lethal concentration (LC50) values of CSMLE, CSGNPs and CSLE were found to be 0.509, 0.340, 0.423 ppm and 0.602, 0.188, 0.646 ppm on Aedes aegypti and Anopheles stephensi, respectively. The findings form an important baseline information proceeding biologically innocuous biopesticide for controlling the malarial and dengue vectors.


Assuntos
Ouro/química , Nanopartículas Metálicas/química , Extratos Vegetais/química , Rutaceae/química , Aedes/efeitos dos fármacos , Aedes/crescimento & desenvolvimento , Animais , Anopheles/efeitos dos fármacos , Anopheles/crescimento & desenvolvimento , Cromatografia Gasosa-Espectrometria de Massas , Inseticidas/síntese química , Inseticidas/química , Inseticidas/toxicidade , Larva/efeitos dos fármacos , Nanopartículas Metálicas/toxicidade , Nanopartículas Metálicas/ultraestrutura , Tamanho da Partícula , Folhas de Planta/química , Folhas de Planta/metabolismo , Rutaceae/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier
6.
Parasitol Res ; 114(4): 1407-15, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25601441

RESUMO

Mosquitoes and mosquito-borne diseases are prone to raise health and economic impacts. Synthetic insecticide-based interventions are indeed in situations of epidemic outbreak and sudden increases of adult mosquitoes. Nanoparticles are being used in many commercial applications and were found that aqueous silver ions can be reduced by an aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. Based on this, silver nanoparticles (SNPs) were synthesized using leaf aqueous extract (LAE) of Mukia maderaspatana. Further, the synthesized SNPs were characterized by UV-visible spectrum, which indicated a strong plasmon resonance at 427 nm. X-ray diffraction (XRD) analysis revealed the average crystalline size of the synthesized SNPs was approximately 64 nm by Debye-Scherrer formulae. Fourier transform infrared (FTIR) spectroscopy analysis revealed the presence of different functional groups like amines, halides, alkanes, alkynes, amides, and esters with respective stretches, which are responsible for the bio-reduction of silver ions. Field emission scanning electron microscopy (FESEM) depicted the spherical morphology of SNPs with size range of 13-34 nm. The larvicidal activity of LAE and SNPs exhibited an effective mortality to Aedes aegypti and Culex quinquefasciatus. The lethal concentration (LC50; LC90) of LAE and SNPs were found to be 0.506; 1.082, 0.392; 0.870 ppm and 0.211; 0.703, 0.094; 0.482 ppm, respectively on A. aegypti and C. quinquefasciatus. Thus, the synthesized SNPs have shown preponderant larvicidal activity, but further studies are needed to formulate the potential larvicidal agents.


Assuntos
Aedes/efeitos dos fármacos , Cucurbitaceae/química , Culex/efeitos dos fármacos , Controle de Insetos , Inseticidas/farmacologia , Nanopartículas Metálicas/química , Extratos Vegetais/farmacologia , Prata/farmacologia , Aedes/crescimento & desenvolvimento , Animais , Culex/crescimento & desenvolvimento , Feminino , Inseticidas/síntese química , Inseticidas/química , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Masculino , Extratos Vegetais/química , Folhas de Planta/química , Prata/química , Difração de Raios X
7.
J Trace Elem Med Biol ; 30: 83-9, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25432487

RESUMO

Tea is an aromatic beverage prepared by pouring boiling water over alleviated leaves of the tea plant. Tea prepared from the aerial parts of Antigonon leptopus has been traditionally used as remedy for cold, diabetes and pain in many countries. The gold nanoparticles (Au NPs) synthesized from powdered leaf extract (decoction) of A. leptopus were characterized by UV­visible spectroscopy (UV­vis), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR), high resolution transmission electron microscopy (HR-TEM), selected area electron diffraction (SAED) pattern and energy dispersive X-ray (EDX) analyses to define the formation of Au NPs. Further, the synthesized Au NPs were well characterized based on their strong surface plasmon resonance (SPR), crystalline nature, functional groups, size and dispersed shapes, purity and Bragg's reflections of face centered cubic (fcc) structure of metallic gold. The Au NPs showed higher free radical scavenging property when compared to the effect of leaf extract. Cytotoxicity study of synthesized Au NPs exhibited the growth inhibitory property at the concentration (GI50) of 257.8 µg/mL in human adenocarcinoma breast cancer (MCF-7) cells after 48 h. Thus, the Au NPs synthesized from the Mexican creeper, A. leptopus revealed the important biological properties: as a free radical as well as anticancer agent. We conclude that the A. leptopus derived biological materials have promising potential as a source for the development of anticancer drug in future.


Assuntos
Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Ouro/farmacologia , Nanopartículas Metálicas/química , Extratos Vegetais/química , Folhas de Planta/química , Compostos de Bifenilo/química , Proliferação de Células/efeitos dos fármacos , Sequestradores de Radicais Livres/química , Humanos , Células MCF-7 , Nanopartículas Metálicas/ultraestrutura , Picratos/química , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
8.
Nat Prod Res ; 29(10): 958-60, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25226215

RESUMO

This study pertains to the phytochemical components and the biological properties of the weed, Antigonon leptopus Hook. & Arn. (AUT/PUS/064). Phytochemical screening of methanolic leaf extract of A. leptopus revealed the presence of saponin, phenolic compounds, tannins, flavonoids, alkaloids, fixed oils and amino acids. Accordingly, 12 phytochemical components were analysed and characterised by GC-MS. Antibacterial activity was evaluated against fish and clinical pathogens. Fish pathogens, Providencia vermicola (MTCC 5578) and Aeromonas hydrophila (MTCC 646) were more sensitive to the methanolic leaf extract than clinical pathogens. A useful information was obtained from the phytochemistry of A. leptopus leaves, which would pave way to further applications to treat fish diseases and for utility in the pharmaceutical field.


Assuntos
Aeromonas hydrophila/efeitos dos fármacos , Antibacterianos/química , Extratos Vegetais/química , Polygonaceae/química , Providencia/efeitos dos fármacos , Animais , Antibacterianos/isolamento & purificação , Peixes/microbiologia , Cromatografia Gasosa-Espectrometria de Massas , Folhas de Planta/química
9.
Ecotoxicol Environ Saf ; 107: 220-8, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25011118

RESUMO

The aim of the present study was to assess the oxidative stress, antioxidant response and histopathological changes of nickel nanoparticles (Ni NPs) exposure (14 days) in Mozambique tilapia, Oreochromis mossambicus. Ni NPs were synthesized by metal salt reduction method and characterized by X-ray diffraction (XRD) and Transmission electron microscopy (TEM). The XRD peaks at 44°, 51° and 76° were indexed to the (111), (200) and (220) Bragg's reflections of cubic structure of Nickel, respectively. The crystallite sizes were calculated using Scherrer's formula applied to the major intense peaks and found to be the size of 56nm. TEM images showed that the synthesized Ni NPs are spherical in shape. Biochemical analysis indicated that the superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity was significantly affected by Ni NPs treated O. mossambicus. Reduced antioxidant enzymes and the contents of antioxidants were lowered in the liver and gills of fishes treated with Ni NPs. After 14 days of exposure, a significant accumulation of Ni in the Ni NPs in experimental group was observed in the gill and skin tissues, with the highest levels found in the liver. Ni NPs exposed fish showed nuclear hypertrophy (NH), nuclear degeneration (ND), necrosis (NC) and irregular-shaped nuclei were observed in liver tissue. The hyperplasia of the gill epithelium (GE), lamellar fusion of secondary lamellae (LF), dilated marginal channel (MC), epithelial lifting (EL) and epithelial rupture were observed in gill tissue. Degeneration in muscle bundles (DM), focal area of necrosis (NC) vacuolar degeneration in muscle bundles (VD), edema between muscle bundles (ED) and splitting of muscle fibers were noticed in skin tissue. Further ecotoxicological evaluation will be made concerning the risk of Ni NPs on aquatic environment.


Assuntos
Nanopartículas Metálicas/toxicidade , Níquel/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Tilápia/metabolismo , Animais , Antioxidantes/metabolismo , Catalase/metabolismo , Brânquias/efeitos dos fármacos , Fígado/efeitos dos fármacos , Nanopartículas Metálicas/química , Níquel/análise , Níquel/química , Superóxido Dismutase/metabolismo , Difração de Raios X
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...