RESUMO
Formalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetic causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a biotynilated probe one positive case was detected. Using 32P-labelled probes in a dot blot assay format, five further positives were obtained. These were all confirmed as positive by a nested polymerase chain reaction assay. Electron microscopy revealed virus in all these five positive cases. The six B19 DNA positive cases of hydrops fetalis were from 1974, 1980, 1982, 1987 and 1988, four of which occurred during the second half of the year, confirming the seasonality of the disease.
Assuntos
Feto/virologia , Hidropisia Fetal/virologia , Parvovirus B19 Humano/isolamento & purificação , Brasil , HumanosRESUMO
Pulmonary lesions compatible with adenovirus infection were detected by gross and microscopic examination of autopsy tissues children aged from 5 to 34 months. Hepatic lesions indicative of systemic infection were also found in four of the chisldren. The viral etiology was confirmed in three cases by in-situ hibridization, electro-microscopy and immunofluorescence performed in parafin-embedded tissues, and in one case by cell culture isolation of adenovirus type 2 from nasopharyngeal exudate. Routine testing by methods additional to conventional light microscopy would probably have revealed a larger number of adenovirus infections among the 1.103 autopsy records analyzed in this study
Assuntos
Criança , Humanos , Infecções por Adenoviridae , Infecções por Adenovirus Humanos , Pneumonia Viral/etiologiaRESUMO
Pulmonary lesions compatible with adenovirus infection were detected by gross and microscopic examination of autopsy tissues from children aged from 5 to 34 months. Hepatic lesions indicative of systemic infection were also found in four of the children. The viral etiology was confirmed in three cases by in-situ hybridization, electron-microscopy and immunofluorescence performed in paraffin-embedded tissues, and in one case by cell culture isolation of adenovirus type 2 from nasopharyngeal exudate. Routine testing by methods additional to conventional light microscopy would probably have revealed a larger number of adenovirus infections among the 1,103 autopsy records analyzed in this study.