RESUMO
During serotyping of fecal specimens positive for rotavirus from hospitalized diarrhea patients in Pune, India, about 10% showed multireactivity in enzyme immunoassay with monoclonal antibodies specific for serotypes G1-4, 6, 8, and 10. In order to characterize some of these, three fecal specimens from children and one from adult were culture adapted. All the isolates showed long RNA pattern, but three out of four isolates belonged to subgroup I and II and one, to subgroup I. The isolates were confirmed as G6 by neutralization assay and polymerase chain reaction. Nucleotide sequences of cDNA derived from the gene encoding the outer capsid protein, VP7 of two strains indicated >94% identity with G6, the serotype, generally associated with cattle. The isolates were more close to G6 RF strain, which is a bovine rotavirus, reported from France. This is a first report of isolation of bovine serotype, G6 from children as well as adults from India.
Assuntos
Diarreia/virologia , Infecções por Rotavirus/virologia , Rotavirus/classificação , Adulto , Proteínas do Capsídeo/genética , Criança Hospitalizada , Fezes/microbiologia , Humanos , Índia/epidemiologia , Lactente , RNA Viral/análise , Rotavirus/genética , Rotavirus/isolamento & purificação , Infecções por Rotavirus/epidemiologia , Alinhamento de Sequência , Análise de SequênciaRESUMO
BACKGROUND & OBJECTIVES: Rotavirus is the major cause of gastroenteritis in infants and young children all over the world. The objective of the study was to develop a rapid ELISA for the diagnosis of rotavirus infection in children hospitalised with diarrhoea. METHODS: Immune serum was raised in rabbits by inoculating semipurified rotavirus, SA-11 strain. Immunoglobulins were conjugated to horse radish peroxidase and a rapid ELISA for rotavirus diagnosis was developed. The rapid ELISA was compared with routine ELISA, developed earlier at NIV. RESULTS: Of the 155 faecal samples from patients with diarrhoea, 96 were positive by rapid ELISA and 95 in routine NIV ELISA. OD values were higher in rapid ELISA. The rapid ELISA takes only 4 h to complete. INTERPRETATION & CONCLUSION: Rotavirus diagnosis by rapid ELISA is simple and easy to perform. This may lead to a significant reduction in the unnecessary usage of antibiotics, which cannot control infection due to rotavirus. This technology is being commercialized.
