RESUMO
Protease enzyme has lot of commercial applications, so the cost-effective production of protease using sunflower oil seed waste was carried out from Oerskovia xanthineolyitca NCIM 2839. The maximum protease production was after 24 h of incubation with 2.5 % oil seed waste concentration. O. xanthineolytica was found to produce two proteases-P1 and P2. The proteases were purified using 60 % cold acetone precipitation and DEAE-cellulose ion exchange chromatography. SDS-PAGE revealed molecular weight of P1 and P2 was 36 and 24 kDa, respectively. P1 and P2 were optimally active at pH 7.0 and pH 7.5 at temperature 35 and 40 °C, respectively. Analysis of hydrolyzed product of P1 and P2 by HPLC reveals that the P1 has endoprotease and P2 has exoprotease activity. The treated soy milk with immobilized proteases showed increased shelf life and removal of off flavor.
RESUMO
Papad is familiarly known as an Indian food adjunct or snack food. In this study, attempts have been made to increase the protein, minerals and crude fibre content of the papad with incorporation of Pleurotus sajor-caju powder to develop a novel snack food. Mushroom powder was added in different proportions with other ingredients of the papad and the prepared papad was subjected to physicochemical and sensorial analyses. Mushrooms pretreated with 1% potassium metabisulphite followed by whey treatment had better colour and reduced drying time. Drying at 50°C produced more amount of mushroom powder (350 µ) and had higher acceptability of rehydrated samples. The papad enriched with 20% mushroom powder showed increased values of protein (15.8%), minerals (38.87%) and crude fibre (218.18%) content and had maximum sensory score. Therefore, papad supplemented with oyster mushroom powder may help to reduce protein malnutrition, constipation and mineral deficiency disorders.
