1.
Cold Spring Harb Protoc
; 2015(12): pdb.corr091009, 2015 Dec 02.
Artigo
em Inglês
| MEDLINE
| ID: mdl-26631118
2.
Cold Spring Harb Protoc
; 2014(12): 1259-66, 2014 Oct 23.
Artigo
em Inglês
| MEDLINE
| ID: mdl-25342069
RESUMO
The avian brain is a valuable model for exploring adult neurogenesis. Here we use immunohistochemical methods to detect cell division and the incorporation of new neurons in the adult zebra finch brain. The nonradioactive, relatively inexpensive thymidine analog bromodeoxyuridine (BrdU) is used to label replicating DNA in dividing cells. The brain is harvested, fixed, and dehydrated before being embedded in polyethylene glycol (PEG), which results in superior histology compared to frozen specimens. After the PEG-embedded brain tissue is sectioned and mounted on slides, standard immunohistochemical procedures are used to detect both BrdU and the neuron-specific marker Hu.