RESUMO
A linkage map consisting of 158 DNA markers were constructed by using a recombinant inbred line (RIL) population derived from the indica-indica rice cross Zhenshan 97B x Milyang 46. Quantitative trait loci (QTLs) conditioning grain yield and five yield component traits were determined at the one-locus and two-locus levels, and genotype-by-environment (GE) interactions were analyzed. Thirty-one QTLs were detected to have significant additive effects for yield traits, of which 12 also exhibited significant epistatic effects. Sixteen significant additive-by-additive (AA) interactions were detected, of which nine occurred between QTLs with own additive effects (M(ep)QTLs), four occurred between QTLs showing epistatic effects only (epQTLs), and three occurred between M(ep)QTLs and epQTLs. Significant GE interactions were found for six QTLs with additive effects and one AA interaction. Generally, the contributions to the phenotypic variation were higher due to QTL main effects than to epistatic effects. The detection of additive effects and AA effects of a QTL interfered with each other, indicating that the detection of QTLs with main effects, as well as the magnitude and directions of the additive effects, might vary depending on their interactions with other loci.
RESUMO
In a population consisting of 227 test-crosses of Zhenshan 97A x (Zhenshan 97B x Milyang 46) F6, a linkage map of 115 RFLP markers was constructed and employed to detect QTL for rice CMS-WA (wild abortive cytoplasmic male sterility) fertility restoration. One QTL having major effect, qRf-10, and 3 QTL having minor effects, qRf-1, qRf-7 and qRf-11, were detected. The four genes mainly acted additively with each other, but epistasis was also evident. Two minor QTL, qRf-1 and qRf-11, were significantly interacted with the major gene qRf-10. It was also found that interaction effects among minor QTL varied greatly depending on whether qRf-10 was present. Two other QTL, qSF-1 and qSF-7, were detected, which did not display any effects on fertility restoration. Instead, they showed effects for increasing SF when qRf-10 was present.
