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1.
J Neurobiol ; 49(2): 129-41, 2001 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-11598920

RESUMO

Factors in the environment appear to be responsible for inducing many of the cell fates in the retina, including, for example, photoreceptors. Further, there is a conserved order of histogenesis in the vertebrate retina, suggesting that a temporal mechanism interacts in the control of cellular determination. The temporal mechanism involved could result from different inducing signals being released at different times. Alternatively, the inducing signals might be present at many stages, but an autonomous clock could regulate the competence of cells to respond to them. To differentiate between these mechanisms, cells from young embryonic retinas were dissociated and grown together with those from older embryos, and the timing of photoreceptor determination assayed. Young cells appeared uninfluenced by older cells, expressing photoreceptor markers on the same time schedule as when cultured alone. A similar result was obtained when the heterochronic mixing was done in vivo by grafting a small plug of optic vesicle from younger embryos into older hosts. Even the graft cells at the immediate margin of the transplant failed to express photoreceptor markers earlier than normal, despite their being in contact with older, strongly expressing host cells. We conclude that retinal progenitors intrinsically acquire the ability to respond to photoreceptor-inducing cues by a mechanism that runs on a cell autonomous schedule, and that the conserved order of histogenesis is based in part on this competence clock.


Assuntos
Indução Embrionária , Células Fotorreceptoras de Vertebrados/citologia , Retina/embriologia , Xenopus laevis/embriologia , Fatores Etários , Animais , Agregação Celular , Diferenciação Celular , Linhagem da Célula , Transplante de Células , Células Cultivadas , Técnicas de Cocultura , Embrião não Mamífero/citologia , Feminino , Larva , Masculino , Técnicas de Cultura de Órgãos , Células Fotorreceptoras de Vertebrados/transplante , Retina/citologia , Xenopus laevis/crescimento & desenvolvimento
2.
Invest Ophthalmol Vis Sci ; 41(9): 2684-8, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10937583

RESUMO

PURPOSE: To examine the role of ocular sympathetic activity in the enlargement of the rabbit eyeball during postnatal growth. METHODS: Fourteen New Zealand albino rabbits aged 5 weeks underwent unilateral surgical transection of the cervical sympathetic trunk caudal to the superior cervical ganglion. Postoperative enlargement of both eyeballs was monitored by measuring the axial length and corneal diameters every 2 weeks for 22 weeks (7-27 weeks of age). Rabbits were housed under a 12-hour light/12-hour dark cycle, and the measurements were made in the middle of the light period. At a final age of 30 to 31 weeks, the refractive state of the whole eye was determined on both sides by measurement through the central cornea with a refractometer. Rabbits were then killed, eyeballs enucleated, and their ocular volumes determined. RESULTS: From 9 weeks of age the axial length and corneal diameters were significantly shorter (P < 0.05) in the decentralized eye (surgical side) compared with the intact eye. This reduction remained statistically significant throughout the study period. However, the final refractive states of the two eyes were found not to be different. The mean ocular volume determined after postmortem enucleation was 4.5% less in the decentralized eye than in the intact eye (P < 0.05). CONCLUSIONS: Sympathetic nervous system activity is involved in the normal enlargement of the rabbit eyeball during postnatal growth. However, removal of the ocular sympathetic tone at the age of 5 weeks does not significantly alter the refractive state of the eye when measured in young adulthood.


Assuntos
Olho/patologia , Sistema Nervoso Simpático/fisiopatologia , Envelhecimento/fisiologia , Animais , Animais Recém-Nascidos , Antropometria , Córnea/patologia , Córnea/fisiopatologia , Olho/fisiopatologia , Hipertrofia/fisiopatologia , Coelhos , Erros de Refração/fisiopatologia , Simpatectomia , Sistema Nervoso Simpático/cirurgia
3.
Mech Dev ; 94(1-2): 25-36, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10842056

RESUMO

Fibroblast growth factor-8 (FGF-8) is an important signaling molecule in the generation and patterning of the midbrain, tooth, and limb. In this study we show that it is also involved in eye development. In the chick, Fgf-8 transcripts first appear in the distal optic vesicle when it contacts the head ectoderm. Subsequently Fgf-8 expression increases and becomes localized to the central area of the presumptive neural retina (NR) only. Application of FGF-8 has two main effects on the eye. First, it converts presumptive retinal pigment epithelium (RPE) into NR. This is apparent by the failure to express Bmp-7 and Mitf (a marker gene for the RPE) in the outer layer of the optic cup, coupled with the induction of NR genes, such as Rx, Sgx-1 and Fgf-8 itself. The induced retina displays the typical multilayered cytoarchitecture and expresses late neuronal differentiation markers such as synaptotagmin and islet-1. The second effect of FGF-8 exposure is the induction of both lens formation and lens fiber differentiation. This is apparent by the expression of a lens specific marker, L-Maf, and by morphological changes of lens cells. These results suggest that FGF-8 plays a role in the initiation and differentiation of neural retina and lens.


Assuntos
Proteínas Aviárias , Proteínas do Olho , Olho/embriologia , Fatores de Crescimento de Fibroblastos/genética , Fatores de Crescimento de Fibroblastos/metabolismo , Fator de Crescimento Transformador beta , Animais , Proteína Morfogenética Óssea 7 , Proteínas Morfogenéticas Ósseas/genética , Proteínas Morfogenéticas Ósseas/metabolismo , Diferenciação Celular/genética , Embrião de Galinha , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Indução Embrionária/genética , Fator 8 de Crescimento de Fibroblasto , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/genética , Proteínas com Homeodomínio LIM , Cristalino/embriologia , Fatores de Transcrição Maf , Mesoderma , Fator de Transcrição Associado à Microftalmia , Proteínas do Tecido Nervoso/genética , Fator de Transcrição PAX6 , Fatores de Transcrição Box Pareados , Epitélio Pigmentado Ocular/embriologia , Proteínas Repressoras , Retina/embriologia , Fatores de Transcrição/metabolismo
4.
Neuron ; 28(3): 779-92, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11163266

RESUMO

Axon pathfinding relies on the ability of the growth cone to detect and interpret guidance cues and to modulate cytoskeletal changes in response to these signals. We report that the murine POU domain transcription factor Brn-3.2 regulates pathfinding in retinal ganglion cell (RGC) axons at multiple points along their pathways and the establishment of topographic order in the superior colliculus. Using representational difference analysis, we identified Brn-3.2 gene targets likely to act on axon guidance at the levels of transcription, cell-cell interaction, and signal transduction, including the actin-binding LIM domain protein abLIM. We present evidence that abLIM plays a crucial role in RGC axon pathfinding, sharing functional similarity with its C. elegans homolog, UNC-115. Our findings provide insights into a Brn-3.2-directed hierarchical program linking signaling events to cytoskeletal changes required for axon pathfinding.


Assuntos
Axônios/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas de Homeodomínio , Fatores de Transcrição/metabolismo , Vias Visuais/embriologia , Vias Visuais/metabolismo , Animais , Axônios/ultraestrutura , Comunicação Celular/genética , Embrião de Galinha , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/farmacologia , Perfilação da Expressão Gênica/métodos , Cones de Crescimento/metabolismo , Proteínas com Domínio LIM , Camundongos , Camundongos Knockout , Proteínas dos Microfilamentos/genética , Proteínas dos Microfilamentos/metabolismo , Dados de Sequência Molecular , Nervo Óptico/patologia , Doenças do Nervo Óptico/genética , Doenças do Nervo Óptico/patologia , Estrutura Terciária de Proteína , Retina/patologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/metabolismo , Homologia de Sequência de Aminoácidos , Transdução de Sinais/genética , Colículos Superiores/citologia , Colículos Superiores/embriologia , Colículos Superiores/metabolismo , Fator de Transcrição Brn-3B , Fatores de Transcrição/genética , Fatores de Transcrição/farmacologia , Vias Visuais/citologia
5.
Semin Cell Dev Biol ; 9(3): 241-7, 1998 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9665858

RESUMO

The retina is a favorite model for studying determination and differentiation in the central nervous system because of its ready accessibility, diversity of cell types and regular organization. Many molecules which act as potential inducers of cell fate have been identified by exposing progenitors to them and assaying their differentiation. In addition, heterochronic transplants demonstrate that regulation of cellular competence (i.e. the ability of progenitors to respond to inducers) plays an important role in differentiation. The neurogenic genes Delta and Notch acting as ligand and receptor, respectively, play a role in regulating cell competence by normally inhibiting progenitors from differentiating. Misexpression of an activated form of Notch 'freezes' progenitors in an undifferentiated, neuroepithelial state. Conversely, progenitors failing to be inhibited, either by their own overexpression of Delta, or by a dominant-negative Delta construct which blocks signaling, adopt the earliest fates generated in the retina (i.e. cones and ganglion cells). We suggest that retinal progenitors use lateral inhibition mediated by Delta-Notch to regulate their competence to respond to inductive cues in a changing environment. Such signaling is essential for formation of the proper cell types in appropriate numbers at the right stage of development to make functional circuits.


Assuntos
Indução Embrionária , Proteínas de Membrana/fisiologia , Retina/embriologia , Transdução de Sinais/fisiologia , Animais , Proteínas do Olho/análise , Transplante de Tecido Fetal , Peptídeos e Proteínas de Sinalização Intracelular , Proteínas de Membrana/genética , Morfogênese , Células Fotorreceptoras/embriologia , Receptores Notch , Retina/citologia , Retina/transplante , Células-Tronco , Xenopus laevis
6.
Nature ; 385(6611): 67-70, 1997 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-8985247

RESUMO

To generate the variety of mature neurons and glia found in the developing retina, the competence of pluripotent progenitor cells to respond to extracellular signals must be controlled. Delta, a ligand of the Notch receptor, is a candidate for regulating progenitor competence on the grounds that activation of the pathway involving Notch and Delta can inhibit cellular differentiation. Here we test this possibility in the developing Xenopus retina by misexpression of Delta messenger RNA. We find that Delta-misexpressing cells with wild-type neighbours adopt earlier fates, primarily becoming ganglion cells and cone photoreceptors. Progenitors transfected with Delta later in development also produce rod photoreceptors, but not the latest-generated cell types, demonstrating the importance of timing in Delta function. We conclude that Delta signalling in the vertebrate retina is a basic regulatory mechanism that can be used to generate neuronal diversity.


Assuntos
Proteínas de Membrana/metabolismo , Neurônios/fisiologia , Retina/citologia , Transdução de Sinais , Animais , Diferenciação Celular/fisiologia , Células Clonais , Peptídeos e Proteínas de Sinalização Intracelular , Proteínas de Membrana/genética , RNA Mensageiro , Receptores de Superfície Celular/metabolismo , Receptores Notch , Retina/embriologia , Células-Tronco/fisiologia , Transfecção , Xenopus
7.
Nature ; 381(6583): 603-6, 1996 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-8637595

RESUMO

The neurally expressed genes Brn-3.1 and Brn-3.2 (refs 1-6) are mammalian orthologues of the Caenorhabditis elegans unc-86 gene that constitute, with Brn-3.0 (refs 1-3,8,9), the class IV POU-domain transcription factors. Brn-3.1 and Brn-3.2 provide a means of exploring the potentially distinct biological functions of expanded gene families in neural development. The highly related members of the Brn-3 family have similar DNA-binding preferences and overlapping expression patterns in the sensory nervous system, midbrain and hindbrain, suggesting functional redundancy. Here we report that Brn-3.1 and Brn-3.2 critically modulate the terminal differentiation of distinct sensorineural cells in which they exhibit selective spatial and temporal expression patterns. Deletion of the Brn-3.2 gene causes the loss of most retinal ganglion cells, defining distinct ganglion cell populations. Mutation of Brn-3.1 results in complete deafness, owing to a failure of hair cells to appear in the inner ear, with subsequent loss of cochlear and vestibular ganglia.


Assuntos
Proteínas de Ligação a DNA/fisiologia , Orelha Interna/embriologia , Desenvolvimento Embrionário e Fetal/fisiologia , Olho/embriologia , Proteínas de Homeodomínio , Fatores de Transcrição/fisiologia , Animais , Diferenciação Celular/genética , Diferenciação Celular/fisiologia , Proteínas de Ligação a DNA/genética , Surdez/embriologia , Surdez/genética , Desenvolvimento Embrionário e Fetal/genética , Deleção de Genes , Células Ciliadas Auditivas/anormalidades , Células Ciliadas Auditivas/embriologia , Hibridização In Situ , Camundongos , Família Multigênica , Retina/embriologia , Células Ganglionares da Retina/citologia , Fator de Transcrição Brn-3B , Fatores de Transcrição/genética
8.
Perspect Dev Neurobiol ; 3(3): 147-59, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8931090

RESUMO

A cardinal event in the development of all brain structures is the time at which progenitor cells leave the cell cycle and begin to differentiate. We examined cell genesis in the retina of the macaque monkey (Macaca mulatta) by labeling dividing cells with radioactive thymidine ([3H]TdR) and following their fate at terminal division by virtue of their remaining radiolabeled after a long survival period. A number of distinct patterns of cell genesis were observed. The two tissues generated by the optic vesicle, the retinal pigment epithelium and neuroretina, share closely coincident temporal and spatial patterns of cell genesis, indicating that this process may be controlled by a common mechanism. Although overlapping to varying degrees, a clear sequence of genesis was revealed between specific cell types within the neuroretina: ganglion cells are generated first, followed by horizontal cells, cone photoreceptors, amacrine cells, Müller cells, bipolar cells, and, finally, rod photoreceptors. Retinal ganglion cells of differing soma diameter are born at different times-the smallest cells are generated early, the largest late, suggesting a further refined sequence of the functional classes of monkey retinal ganglion cells (first P gamma, then P beta, last P alpha). In addition, at sites where a homogeneous population of cells are crowded and stacked on top of each other (the foveola and perifovea for cones and ganglion cells, respectively) there is a vitreal-to-scleral intralaminar pattern of [3H]TdR labeled cell placement, which reflects both time of genesis and pattern of movement during foveation. These gradients suggest several scenarios for cell fate specification in the retina, many of which might not be obvious in mammals that develop more quickly and have less specialized retinal structure. Thus, data from the highly specialized and slowly developing macaque retina can help to understand visual development in humans and indicate useful avenues for future experimental studies in other species.


Assuntos
Macaca mulatta/embriologia , Retina/embriologia , Animais , Diferenciação Celular , Linhagem da Célula , Morfogênese , Epitélio Pigmentado Ocular/citologia , Epitélio Pigmentado Ocular/embriologia , Retina/citologia
9.
J Comp Neurol ; 363(3): 359-76, 1995 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-8847405

RESUMO

The development of the retinal pigment epithelium (RPE) was studied in rhesus monkey (Macaca mulatta) fetuses, neonates, and juveniles exposed to a pulse of 3H-thymidine (3H-TdR) between embryonic day (E) 25 and postnatal day (P) 204 and examined at short and long intervals after the injection of the isotope. The RPE develops from the outer layer of the optic cup which by E45 consists of a multistratified epithelium. The outer layer appears immature near the retina's edge and gradually becomes monostratified and more mature centrally. Even at this early stage, all cells contain pigmented melanosomes, although peripherally the pigment is limited to the apical portion of the cells. Examination of autoradiograms from animals allowed to survive for several postnatal months shows that monkey RPE cell genesis begins just after E27, increasing to a peak frequency of 0.38 cells/mm at E43. Between E30 and E85 the density of radiolabelled cells varies within a restricted range of from 0.2 to 0.4 cells/mm (mean = 0.25 +/- 0.09). From the density of radiolabelled cells, and data on the overall density of RPE cells in the juvenile retina, we determined the labelling index. During the first half of gestation, between 0.38% and 0.99% (mean = 0.65 +/- 0.22) of RPE cells are generated during the short interval of isotope availability after pulse injection. Approximately 5% of RPE cells were generated by E33, and 50% by E71. After E85, RPE cytogenesis begins gradually to decrease, and 95% of the cells have been generated by the time of birth. Continued, very low density (0.01 cells/mm) cytogenesis in the RPE is seen at P17, and persists at least until seven months postnatally. RPE cell genesis begins near the fovea, and proceeds towards the periphery. Cell division largely ceases in both foveal and perifoveal regions by E56, at which time labelled cells first begin to appear peripheral to the equator. Besides the timing differences, RPE genesis in the central retina differs from that in the peripheral retina in that it proceeds at a higher rate, and lasts for a shorter time period. A prolonged postnatal period of low density RPE cell genesis persists in both central and peripheral retina. Comparison of the pattern of expansion of the area containing radiolabelled cells in the RPE and neuroretina demonstrates a remarkable spatial and temporal correspondence. Close analysis suggests that at any point on the retina, the last cells are generated in the neuroretina slightly before the last cells in the RPE.


Assuntos
Epitélio Pigmentado Ocular/embriologia , Animais , Autorradiografia , Diferenciação Celular , Feminino , Macaca mulatta , Epitélio Pigmentado Ocular/citologia , Epitélio Pigmentado Ocular/metabolismo , Gravidez , Retina/embriologia , Retina/metabolismo , Timidina/metabolismo
10.
Neuron ; 14(3): 487-96, 1995 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-7695895

RESUMO

The neurogenic gene Xotch acts to divert cellular determination during gastrulation in Xenopus embryos. We examined the role of Xotch in the developing retina, where cell signaling events are thought to affect differentiation. Xotch is expressed in undifferentiated precursor cells of the ciliary marginal zone and late embryonic central retina. It is not expressed in stem cells or in differentiated neurons and glia. Expression in the retina is spatially restricted even in the absence of cell division. The final Xotch-positive precursor cells in the central retina mostly differentiate as Müller glia, suggesting that this is the last available fate of cells in the frog retina. Transfection of an activated form of Xotch into isolated retinal cells causes them to retain a neuroepithelial morphology, indicating that the continued activation of Xotch inhibits cell differentiation.


Assuntos
Envelhecimento/fisiologia , Diferenciação Celular/genética , Gástrula/fisiologia , Expressão Gênica , Neurônios/fisiologia , Retina/embriologia , Animais , Afidicolina/farmacologia , Divisão Celular/efeitos dos fármacos , Embrião não Mamífero/fisiologia , Gástrula/citologia , Hidroxiureia/farmacologia , Imuno-Histoquímica , Hibridização In Situ , Neuroglia/citologia , Neuroglia/fisiologia , Neurônios/citologia , Retina/citologia , Retina/crescimento & desenvolvimento , Transdução de Sinais , Xenopus
11.
J Comp Neurol ; 322(4): 577-88, 1992 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-1401251

RESUMO

We have analyzed the genesis of various neuronal classes and subclasses in the ganglion cell layer of the primate retina. Neurons were classified according to their size and the time of their origin was determined by pulse labeling with 3H-thymidine administered to female monkeys 38 to 70 days pregnant. All offspring were sacrificed postnatally, and their retinas processed for autoradiography. The somata of cells in the retinal ganglion cell layer generated on embryonic day (E) 38 ranged from 9 to 14 microns in diameter. Between E40 and E56, the minimum soma diameter remained around 8-9 microns, while the maximum gradually increased to 22 microns. As a consequence, the means of the distributions of labeled cells also increased with age, from 11.8 microns diameter for cells generated on E38 to 14.6 microns diameter at E56. Over this period the percentage of labeled cells in the 10.5-16.5 microns and greater than 16.5 microns diameter range gradually increased. The proportion of the labeled cells in the less than 10.5 microns diameter range decreased from E38 to E45, but subsequently increased rapidly. At the end of neurogenesis in the retinal ganglion cell layer, around E70, most labeled cells were considerably smaller (7-9 microns) than those generated earlier. Our results indicate that within the ganglion cell layer of the macaque, neurons of small caliber are generated first, followed successively by medium sized cells. Large, putative P alpha cells are generated late. The production between E56 and E70 of cells with the smallest somata suggests that the last-generated neurons in the ganglion cell layer are predominantly displaced amacrine cells. Within the same sector of retina, different classes of neurons in the ganglion cell layer of the rhesus monkey appear to have a sequential schedule of production.


Assuntos
Macaca mulatta/embriologia , Retina/embriologia , Células Ganglionares da Retina/citologia , Animais , Idade Gestacional , Morfogênese , Retina/citologia
12.
J Neurosci Methods ; 41(3): 231-8, 1992 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-1513182

RESUMO

Difficulty encountered in resolving grains of exposed photographic emulsion in autoradiographs of the densely melanized retinal pigment epithelium was solved by using epi-polarized or incident light microscopy. The apparatus used included a metallurgical illuminator specifically designed for epi-polarization microscopy or, as a less expensive but only slightly less effective alternative, a modified fluorescence illuminator. The black melanin granules absorb incident light (as they do in vivo) while the silver grains reflect it producing a "darkfield-like" representation. Brightfield and darkfield-like images can be alternated easily and quickly, or both can be viewed simultaneously. Epi-polarization microscopy has wider application in resolving a reflective label over any opaque background staining or dark second label.


Assuntos
Autorradiografia/métodos , Metais/análise , Microscopia de Polarização/métodos , Microscopia/métodos , Gambás/anatomia & histologia , Animais , Histocitoquímica , Luz , Substância Cinzenta Periaquedutal/anatomia & histologia , Retina/anatomia & histologia , Retina/citologia , Células Ganglionares da Retina/ultraestrutura
13.
J Comp Neurol ; 312(3): 341-52, 1991 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-1748737

RESUMO

Cytogenesis in the postnatal cat retina was studied with the aid of 3H-thymidine autoradiography to identify the cell classes generated. Cells proliferate in two stages, which are separate spatially and temporally. Previous studies have shown that during Stage 1, cytogenesis occurs at high density at the ventricular surface of the retina, whereas Stage 2 occurs at low density in the inner retinal layers. At the ages studied, the progeny of Stage 1 cytogenesis are distributed in an annulus toward the margin of the retina, and those of Stage 2 occur central to the annulus, indicating that Stage 2 follows Stage 1. Cell genesis in Stage 1 appears to cease by P16; genesis in Stage 2 persists until between P21 and P30. The same cell classes (amacrine cells, bipolar cells, Müller cells, and rod photoreceptors) are generated during both Stages 1 and 2, but there are significant changes in their proportions both within and between stages. The proportion of the Stage 1 mitoses that form bipolar cells increases from 31% at P0 to 62% at P14. A corresponding decrease is observed in the proportion of rods (from 60% at P0 to 32% at P14). The proportion of cells generated during Stage 2 that become rods increases from 39% at P0 to 70% at P21, whereas the proportion of bipolar cells decreases from 50% at P0 to 23% at P21. Müller cells form a relatively constant proportion (8 to 15%) of the cells generated during both Stage 1 and 2. Thus at the end of Stage 1, mostly bipolar cells are generated; at the end of Stage 2, mostly rods are generated.


Assuntos
Gatos/anatomia & histologia , Retina/citologia , Animais , Animais Recém-Nascidos , Gatos/crescimento & desenvolvimento , Contagem de Células , Divisão Celular/fisiologia , Retina/crescimento & desenvolvimento , Fatores de Tempo
14.
J Comp Neurol ; 309(1): 86-114, 1991 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-1894769

RESUMO

Time of cell origin in the retina of the rhesus monkey (Macaca mulatta) was studied by plotting the number of heavily radiolabeled nuclei in autoradiograms prepared from 2- to 6-month-old animals, each of which was exposed to a pulse of 3H-thymidine (3H-TdR) on a single embryonic (E) or postnatal (P) day. Cell birth in the monkey retina begins just after E27, and approximately 96% of cells are generated by E120. The remaining cells are produced during the last (approximately 45) prenatal days and into the first several weeks after birth. Cell genesis begins near the fovea, and proceeds towards the periphery. Cell division largely ceases in the foveal and perifoveal regions by E56. Despite extensive overlap, a class-specific sequence of cell birth was observed. Ganglion and horizontal cells, which are born first, have largely congruent periods of cell genesis with the peak between E38 and E43, and termination around E70. The first labeled cones were apparent by E33, and their highest density was achieved between E43 and E56, tapering to low values at E70, although some cones are generated in the far periphery as late as E110. Amacrine cells are next in the cell birth sequence and begin genesis at E43, reach a peak production between E56 and E85, and cease by E110. Bipolar cell birth begins at the same time as amacrines, but appears to be separate from them temporally since their production reaches a peak between E56 and E102, and persists beyond the day of birth. Müller cells and rod photoreceptors, which begin to be generated at E45, achieve a peak, and decrease in density at the same time as bipolar cells, but continue genesis at low density on the day of birth. Thus, bipolar, Müller, and rod cells have a similar time of origin. The maximal temporal separation of cell birth is between cones and amacrine cells so that cell generation exhibits two relatively distinct phases: the first phase gives rise to ganglion, horizontal, and cone cells, and the second phase to amacrine, bipolar, rod, and Müller cells. In addition, cells of the first phase are generated faster than the second phase cells, and there are differences in the topography of spread of labeled cells between the two phases. Each cell class displays a central-to-peripheral gradient in genesis, although the spatiotemporal characteristics of the gradients differ between the classes.(ABSTRACT TRUNCATED AT 400 WORDS)


Assuntos
Macaca/crescimento & desenvolvimento , Retina/crescimento & desenvolvimento , Envelhecimento , Animais , Autorradiografia , Replicação do DNA , Idade Gestacional , Macaca/embriologia , Retina/citologia , Retina/embriologia , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/fisiologia , Timidina/metabolismo , Trítio
15.
Vis Neurosci ; 3(1): 21-32, 1989 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2487088

RESUMO

The development of synaptic ribbons in rod and cone photoreceptor terminals of the cat retina was studied using quantitative electron microscopy. At the region of the area centralis, synaptic ribbon profiles are initially recognized at PCD (postconception day) 59. Synaptic ribbon density increases rapidly, reaching a peak of 0.55 ribbons/micron 3 at PCD 68 (postnatal day 3) and maintains approximately that value for an additional 8 d. Following PCD 76, ribbon density begins to decrease, to 0.37 ribbons/microns 3 at PCD 82 and 0.25 ribbons/microns 3 at PCD 102. Although ribbon density drops by approximately 50% during this 39-d period, the outer plexiform layer (OPL) volume at the area centralis increases by about 20%. Ribbon density continues to decrease gradually over a protracted period to reach a final adult value of 0.11-0.14 ribbons/microns 3. During the period of high ribbon density, rod spherules with two, or even three ribbon profiles, were routinely observed. In contrast, in the adult, spherules with more than one ribbon profile are only rarely encountered. During development, the length of synaptic ribbon profiles increases from a mean of 0.22 microns at PCD 62 to the 0.47 microns mean length found in the adult.


Assuntos
Retina/ultraestrutura , Sinapses/ultraestrutura , Animais , Gatos , Contagem de Células , Retina/embriologia , Retina/crescimento & desenvolvimento
16.
Exp Brain Res ; 70(1): 73-8, 1988.
Artigo em Inglês | MEDLINE | ID: mdl-3402569

RESUMO

We have studied the responses of ganglion cells of the cat retina to visual stimulation remote from the center of their receptive field. Following previous work, this response is termed the periphery effect (PE). Cells were identified as Y-, X- or W-class from the latency of their response to optic chiasm stimulation and from their receptive field properties. The strength of the PE elicited by a rotating windmill or counterphased grating stimulus was measured for ganglion cells of all major classes. The PE was consistently stronger in Y- than in X-cells, and the strength of the effect in both X- and Y-cells increased significantly with retinal eccentricity. A PE was elicited from about 47% of W-cells studied. In some (36%) the effect was excitatory, as for X- and Y-cells; in others (11%) it was inhibitory. Despite this heterogeneity, the PE in W-cells increased significantly with eccentricity. These variations of the PE with eccentricity and cell class have implications for the circuitry of the inner plexiform layer.


Assuntos
Retina/fisiologia , Células Ganglionares da Retina/fisiologia , Animais , Fenômenos Biomecânicos , Gatos , Estimulação Luminosa , Tempo de Reação , Análise de Regressão , Células Ganglionares da Retina/classificação
17.
Aust N Z J Ophthalmol ; 13(2): 113-24, 1985 May.
Artigo em Inglês | MEDLINE | ID: mdl-4052260

RESUMO

Cytogenesis in the cat retina was studied using tritiated (3H) thymidine autoradiography and nuclear stains. Three zones of cell division were recognized. In the first zone cell cleavage occurs at the outer limiting membrane. The distribution of these mitotic figures is uniform as early as an embryonic age of 29 days (E29) until E50. At about E50 a "cold spot", made apparent by the absence of mitotic figures, is evident at the site of the developing area centralis. This spreads to encompass the whole retina by postnatal day 10 (P10). A second zone of cell division was recognized by the presence in the developing inner nuclear layer of 3H-thymidine labelled nuclei which do not migrate to the outer limiting membrane (ventricular surface) to divide. Some of these labelled nuclei are located in regions of the retina where cytogenesis at the ventricular surface has ceased. A third zone was observed in the optic nerve fibre/ganglion cell layers from about E54 until beyond the first postnatal month. This activity gives rise to vascular endothelial cells in the nerve fibre/ganglion cell layers. Once established, the developing vascular cells invade the inner plexiform and inner nuclear layers to form the deeper capillary net.


Assuntos
Retina/crescimento & desenvolvimento , Animais , Autorradiografia , Gatos , Divisão Celular , Mitose , Retina/citologia , Retina/embriologia , Vasos Retinianos/citologia , Vasos Retinianos/crescimento & desenvolvimento , Fatores de Tempo
18.
Brain Res ; 351(1): 101-9, 1985 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-3995330

RESUMO

Cytogenesis in the kitten retina has been investigated with [3H]thymidine autoradiography and a stain for mitotic figures. The nuclei of cells in S-phase are located adjacent to the inner margin of the cytoblast layer, near the inner plexiform layer. The nuclei then migrate toward the outer limiting membrane (OLM) to divide. Evidence is presented that in a small population of mitotic cells, the nucleus does not migrate to the OLM, but divides in the inner part of the cytoblast layer or, after the outer plexiform layer has formed, in the inner nuclear layer. Cell division in this inner zone begins and ends later than at the OLM. Cells dividing there are fewer in number than those at the OLM, their mitotic spindles are oriented randomly rather than parallel to the retinal surface and their nuclei move little between S- and M-phases of the mitotic cycle. The zones of cytogenesis at the OLM and in the inner cytoblast layer resemble, respectively, the ventricular and subventricular zones of other areas of the developing central nervous system.


Assuntos
Retina/citologia , Animais , Autorradiografia , Gatos , Divisão Celular , Mitose , Retina/crescimento & desenvolvimento , Retina/metabolismo , Timidina/metabolismo
19.
Vision Res ; 25(3): 309-17, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-4024453

RESUMO

In the developing retina of the rabbit the ganglion cell layer can first be identified between E(embryonic day) 20 and 24, but the regional variations found in the adult retina, particularly the visual streak, are not well developed until shortly before birth. At about E31, the last day of gestation, the laminar structure of the retina begins to mature, cytogenesis begins to cease and the outer plexiform layer starts to form. These processes commence in far temporal retina, at or near the site of the area centralis, and spread preferentially along the visual streak.


Assuntos
Retina/embriologia , Animais , Contagem de Células , Sobrevivência Celular , Idade Gestacional , Mitose , Coelhos , Células Ganglionares da Retina/citologia
20.
Neuroscience ; 11(2): 289-301, 1984 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-6425714

RESUMO

In many mammals, particularly species with frontalised eyes, a small region o retina is strongly specialised for high resolution, binocular vision. The region is typically located near the centre of the retina, a few millimetres temporal to the optic disc, and is termed the "area centralis" or, in some primates in which the specialisation is particularly well developed, the "fovea centralis". Where the specialisation is well developed, the area or fovea centralis dominates the organisation of the adult visual system. Studies of the histogenesis of the retina of the cat indicate that the process of retinal maturation is centred on the area centralis, which thus seems to be an organising focus in the ontogeny as well as the adult function of the visual system.


Assuntos
Fóvea Central/fisiologia , Macula Lutea/fisiologia , Retina/fisiologia , Visão Ocular , Envelhecimento , Animais , Gatos , Embrião de Mamíferos , Fóvea Central/anatomia & histologia , Haplorrinos , Retina/anatomia & histologia , Retina/embriologia , Retina/crescimento & desenvolvimento , Células Ganglionares da Retina/fisiologia , Especificidade da Espécie
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