RESUMO
The A.A. carried out a survey on the functioning of a catering service in Rome. The risk analysis assessment was performed by means of a checklist that collected systematically all data about the environment, the operators, the food and the catering process. The results of the microbiological analysis carried out in the CCP's showed that the general conditions of the catering service were not satisfactory, but also indicated the validity of the checklist used. This method showed a high predictive value and can be useful to improve the application of the HACCP system.
Assuntos
Contaminação de Equipamentos , Contaminação de Alimentos , Microbiologia de Alimentos , Serviços de Alimentação/normas , Clostridium/isolamento & purificação , Utensílios de Alimentação e Culinária , Coleta de Dados , Enterobacteriaceae/isolamento & purificação , Manipulação de Alimentos/normas , Serviços de Alimentação/instrumentação , Mãos/microbiologia , Higiene , Listeria/isolamento & purificação , Refrigeração/normas , Medição de Risco , Cidade de Roma , Staphylococcus aureus/isolamento & purificaçãoRESUMO
The multidrug resistance (mdr) gene family has been shown to encode a membrane glycoprotein, termed the P-glycoprotein, which functions as a drug efflux pump with broad substrate specificity. This multigene family is expressed in a tissue-specific fashion in a wide variety of normal and neoplastic tissues. The regulation of mdr gene expression in normal tissues is not understood. We have recently shown that mdr mRNA and the P-glycoprotein increases dramatically in the secretory luminal and glandular epithelium of the gravid murine uterus. This observation has suggested that mdr gene expression in the uterus is controlled by the physiologic changes associated with pregnancy. This report now demonstrates that mdr mRNA and P-glycoprotein are induced at high levels in the uterine secretory epithelium by the combination of estrogen and progesterone, the major steroid hormones of pregnancy. This regulation of mdr gene expression in the uterus does not require any other contribution from the fetus or placenta. The data indicate that this gene locus is hormonally responsive to estrogen and progesterone in the uterine secretory epithelium, suggesting an important and physiologically regulated role during pregnancy.
