Human papillomavirus serotypes and determinants among women with invasive cervical cancer in Katsina state, Northwest-Nigeria: a multicentre study.

Background: Cervical cancer is the leading cause of gynaecological cancer death among women in developing countries and the most preventable of all gynaecological cancers as its infectious aetiological agent, human papillomavirus (HPV), is known. The knowledge of HPV serotype distribution in a sub-region is key to the implementation of an appropriate HPV vaccination programme. Aim: To assess the prevalence of HPV-DNA, serotypes and risk-determinants among women with invasive cervical cancer (ICC) in Katsina State, Northwestern Nigeria. Methods: This was a cross-sectional, multicenter study involving Federal Teaching Hospital Katsina, General Hospital Katsina and Turai Yar'adua Maternal and Child Hospital Katsina, Nigeria. Sixty-three women with histologically confirmed cervical cancer who fulfilled the criteria were recruited into the study. Tissue blocks with a confirmed diagnosis of ICC were taken to DNA Labs Kaduna for HPV-deoxyribonucleotide acid detection and typing. An interviewer-administered questionnaire developed for the study was used to obtain socio-demographic, reproductive characteristics and the other risk factors for HPV acquisition and persistence. Results: The HPV-positivity rate in ICC was 95.5% while the prevalence of high-risk HPV (Hr-HPV)-DNA in the specimen was 54.6% with 13 HPV-serotypes detected, 9 Hr-HPV types (16,18,31,33,35,45,51,56,82) and 4 low-risk HPV types (6,44,81,89). The most commonly detected HPV serotype among women with a single HPV infection was HPV 81 (40.9%) followed by HPV 16 (28.8%). However, HPV 16 was the most common serotype among those with multiple HPV infections. Prevalence of other detected serotypes were HPV 31 (24.2%), 33 (24.2%), HPV 18 (10.6%), HPV 35 (3.0%), HPV 45 (9.1%), HPV 44 (1.5%), HPV 51 (3.0%), HPV 56 (3.0%), HPV 82 (1.5%), HPV 89 (1.5%) and HPV 6 (1.5%). Forty-four out of 63 women (69.8%) had a single HPV infection, 19 (30.2%) had multiple HPV infections and 15 (24.3%) were co-infected with HPV 16/31/33. There was a statistically significant association between HPV 16 and squamous cell carcinoma (SCC). Conclusion: The study demonstrates a prevalence of HPV-DNA as 95.5% among women with ICC. The most commonly detected HPV serotype was HPV 81 seen in 41% which was an uncommon finding. Furthermore, statistically significant associations between HPV serotypes 16 and 82 with SCC were detected.

A uretheral stone causing urinary obstruction in a paediatric patient.

Urinary retention in pediatric patients is an uncommon presentation, particularly when attributed to urethral stones. We present the case of an 8 year old child who experienced acute urinary retention due to a urethral stone, an infrequent occurrence in this age group. Initial assessment revealed signs of obstructive voiding, prompting further investigation. Imaging studies confirmed the presence of a urethral stone causing complete obstruction. Management involved a multidisciplinary approach, incorporating surgical intervention. This case report underlines the need for awareness about the rare occurrence of urethral stones in pediatric age group which must be kept in mind while treating children.

Cervical cytopathological changes in pregnancy: An experience from a low resource setting.

Background: Cervical cancer is the leading cause of death among women in developing countries. It is preventable through effective cervical cancer screening program. However, in Nigeria, screening programs are opportunistic and coverage is insufficient to make an impact. Aim: This study assessed the cervical cytopathological changes among pregnant women at booking using liquid-based cytology (LBC) in Aminu Kano Teaching Hospital (AKTH). Methodology: This was a cross-sectional study that was carried out at the antenatal Clinic of AKTH, Kano, Nigeria. A total of 161 pregnant women who fulfilled the criteria and gave their consent were recruited into the study using systematic sampling technique at booking for antenatal care. LBC was employed using standard procedure and samples sent to histopathology department for analysis. Pro forma developed for the study was used to obtain the socio-demographic and reproductive characteristics of the women and the risk factors for abnormal cervical cytology. Results: Out of the 161 pregnant women that had cervical cytology screening using LBC on their first prenatal visit during the study, 22 had abnormal cervical cytology, giving a prevalence rate of 13.7%. Out of this, six (27.3%) were atypical squamous cells of undetermined significance, 3 (13.6%) were Atypical Squamous Cells, Cannot Rule Out HSIL (ASC-H), 11 (50.0%) were low-grade Squamous Intraepithelial Lesions while 2 (9.1%) were high grade squamous intraepithelial lesions. Negative smears were seen in 104 women (64.6%). Inflammatory and other conditions of the cervix which are technically negative smears made up the remaining 21.7%. There was a statistically significant association between cervical cytology results and advanced age (P < 0.01), increasing number of lifetime sexual partners since coitarche (P < 0.01), high parity (P < 0.01), absent previous Pap test (P < 0.027), previous history of sexually transmitted infections (P < 0.040), and positive HIV status (P < 0.001). Following binary logistic regression, advanced maternal age, increasing number of sexual partners, high parity, and positive HIV status stood out to be independent predictors of premalignant lesions of the cervix in pregnancy in this study. Conclusion: Advanced maternal age, increasing number of sexual partners, high parity, and positive HIV status stood out to be independent predictors of premalignant lesions of the cervix in the study. Routine cervical cytology screening using LBC should be offered to all antenatal clients in our setting to increase coverage and detection rate of preinvasive lesions of the cervix, and/or pregnant women with increased risk of abnormal cervical cytology from this study.

RésuméContexte: Le cancer du col de l'utérus est la principale cause de décès chez les femmes des pays en développement. Il est évitable grâce à un programme efficace de dépistage du cancer du col utérin. Cependant, au Nigéria, les programmes de dépistage sont opportunistes et la couverture est insuffisante pour avoir un impact. Objectif: Cette étude a évalué les changements cyto-pathologiques cervicaux chez les femmes enceintes lors de la réservation en utilisant la cytologie en phase liquide (LBC) à l'hôpital universitaire Aminu Kano (AKTH). Méthodologie: Il s'agissait d'une étude transversale qui a été réalisée à la clinique prénatale de 'AKTH, Kano, Nigéria. Un total de 161 femmes enceintes qui remplissaient les critères et ont donné leur consentement ont été recrutées dans l'étude en utilisant une technique d'échantillonnage systématique lors de la réservation pour les soins prénatals. LBC a été utilisé en utilisant la procédure standard et des échantillons envoyés au service d'histopathologie pour analyse. Le pro forma développé pour l'étude a été utilisé pour obtenir les caractéristiques sociodémographiques et reproductives des femmes et les facteurs de risque d'une cytologie cervicale anormale. Résultats: Sur les 161 femmes enceintes qui ont subi un dépistage cytologique cervical utilisant le LBC lors de leur première visite prénatale au cours de l'étude, 22 avaient une cytologie cervicale anormale, ce qui donne un taux de prévalence de 13,7%; six (27,3%) étaient des cellules squameuses atypiques de signification indéterminée, 3 (13,6%) étaient des cellules squameuses atypiques, ne peut exclure HSIL (ASC-H), 11 (50,0%) étaient des lésions intraépithéliales squameuses de bas grade tandis que 2 (9,1%) étaient des lésions intraépithéliales squameuses de haut grade. Des frottis négatifs ont été observés chez 104 femmes (64,6%). Les conditions inflammatoires et autres du col de l'utérus qui sont des frottis techniquement négatifs constituaient les 21,7% restants. Il y avait une association statistiquement significative entre l'âge avancé (P <0,01), un nombre croissant departenaires sexuels à vie depuis la coïtarche (P <0,01), parité élevée (P <0,01), absence de test Pap précédent (P <0,027), antécédents d'infections sexuellement transmissibles (P <0,040) et statut VIH positif (P <0,001). Suite à la régression logistique binaire, l'âge maternel avancé, le nombre croissant de partenaires sexuels, une parité élevée et un statut VIH positif se sont révélés être des prédicteurs indépendants des lésions prémalignes du col de l'utérus pendant la grossesse dans cette étude. Conclusion: L'âge maternel avancé, le nombre croissant de partenaires sexuels, la parité élevée et le statut VIH positif se sont révélés être des prédicteurs indépendants des lésions prémalignes du col de l'utérus dans l'étude. Un dépistage cytologique cervical de routine utilisant le LBC doit être proposé à tous les clients prénatals dans notre environnement pour augmenter la couverture et le taux de détection des lésions pré-invasives du col de l'utérus et / ou aux femmes enceintes présentant un risque accru de cytologie cervicale anormale de cette étude.

Academic Stress and Prevalence of Stress-Related Self-Medication among Undergraduate Female Students of Health and Non-Health Cluster Colleges of a Public Sector University in Dammam, Saudi Arabia.

OBJECTIVE: The objective was to report academic stress and prevalence of stress-related self-medication among undergraduate female students of health and nonhealth cluster colleges at a public sector university in Dammam, Saudi Arabia. MATERIALS AND METHODS: A 5-month cross-sectional survey was conducted in the university. The survey included the English version of 10-item Perceived Stress Scale (PSS) to report self-perceived stress. Student responses were analyzed by SPSS version 22 software. RESULTS: The majority of students (85%) perceived examinations as a stressor. Most of the students (64%) had perceived moderate stress that increased as students progressed from preparatory year to 4th year. It declined in students of 5th and 6th year. The prevalence of stress related was reported at 39.58%. Highest prevalence of stress-induced self-medication was reported from College of Nursing (59.09%) and lowest (29.69%) from clinical pharmacy. Most common drug used to self-medicate during stress was caffeine (49.5%). The PSS score was significantly associated with colleges and study levels. CONCLUSION: Students studying in health cluster colleges reported high academic stress and self-medication practice. The major stressors identified were examination and course load. Student counseling sessions and counseling by pharmacists regarding self-care may help in the reduction of such stressors and may promote responsible self-medication. Self-evaluation and quality assurance process of curriculum may highlight areas for improvement in the courses. This may help in lowering academic stress among students.