Characterization and synergistic antibacterial potential of green synthesized silver nanoparticles using aqueous root extracts of important medicinal plants of Pakistan.

In the past few years, biologically synthesized silver nanoparticles (AgNPs) have been standout amongst the most utilized nanoparticles both in the field of therapeutics and clinical practices. Therefore, the current study aimed to synthesize AgNPs for the first time using aqueous root extracts of important plants of Pakistan i.e. Bergenia ciliata, Bergenia stracheyi, Rumex dantatus and Rumex hastatus and characterize them. In addition, antibacterial activity of synthesized AgNPs at 30-150 µg/well was assessed using well diffusion method against Staphylococcus aureus, Staphylococcus haemolyticus, Bacillus cereus, Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa bacterial strains that are considered most harmful bacteria for human beings. The characterization of synthesized AgNPs showed the absorption maxima ranged from 434 to 451 nm and XRD confirmed the crystalline nature of AgNPs as well as FTIR elucidated the involvement of biomolecules for reduction and capping of AgNPs. SEM determined the average size of AgNPs ranging from 25 to 73 nm and strong signals of silver were captured in EDX images. The result of antibacterial activity showed that only aqueous root extracts of all selected plants were inactive against all the tested bacterial strains. However, importantly, direct relationship between zone of inhibition of S. aureus, S. typhi and P. aeruginosa was found with increasing concentration of AgNPs of each selected plant. Moreover, S. haemolyticus was only inhibited by R. hastatus based AgNPs at only high concentrations and E. coli was inhibited by R. dantatus and R. hastatus based AgNPs. However, B. cereus was not inhibited by any AgNPs except R. hastatus and R. hastatus based AgNPs have greater antibacterial potential among all the synthesized AgNPs. These results suggest that synthesized AgNPs have improved antibacterial potential of root extracts of each selected plant and these synthesized AgNPs could be used in pharmaceutical and homeopathic industry for the cure of human diseases.

Evaluation of DNA damage in traffic police wardens of Pakistan due to cadmium and zinc.

Air quality in urban areas is generally poor especially at traffic intersections and roadsides due to continuous vehicular emissions comprising poly aromatic hydrocarbons, heavy metals, benzene, diesel soot etc. The objective of this study was to compare the primary DNA damage in traffic police wardens occupationally exposed to airborne Cd and Zn (exposed group) and educational institution with negligible exposure to airborne Cd and Zn (control group). Blood levels of Cd and Zn in traffic police wardens and control group were determined by Flame Atomic Absorption Spectroscopy (FAAS) and DNA damage was assessed by using Comet assay. The results of this study showed significantly higher amount of Cd (0.18±0.06mgL-1) and Zn (4.87±1.34mgL-1) in the blood of traffic police wardens as compared to control group and according to World Health Organization, these values are 18 and 3 times more to the permissible limit of Cd and Zn respectively in human blood. In addition, significantly higher numbers of DNA damaged cells (28±13%) were observed in traffic police wardens as compared to control group (3.6±2%). Comet tail length was found to be doubled (4.7±1.7µm) in traffic police wardens as compared to the control group (2±1.2µm). These results could be linked to the concentrations of Cd and Zn in blood of traffic police wardens. In conclusion, our results showed that accumulation of Cd and Zn was higher in traffic police wardens due to air pollution (Zn and Cd) and has more damaged DNA of traffic police wardens in Pakistan.

Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals.

BACKGROUND: Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet assay in blood samples collected from industry workers and compare these results with those of classical analytical techniques used for complete blood count analysis. METHODS: Samples from controls (n = 20) and exposed workers (n = 38) from an industrial area in Multan, Pakistan, were subjected to various tests. Malathion residues in blood samples were measured by gas chromatography. RESULTS: The exposed workers who were employed in the pesticide manufacturing industry for a longer period (i.e., 13-25 years) had significantly higher DNA tail length (7.04 µm) than the controls (0.94 µm). Workers in the exposed group also had higher white blood cell and red blood cell counts, and lower levels of mean corpuscular hemoglobin (MCH), MCH concentration, and mean corpuscular volume in comparison with normal levels for these parameters. Malathion was not detected in the control group. However, in the exposed group, 72% of whole blood samples had malathion with a mean value of 0.14 mg/L (range 0.01-0.31 mg/L). CONCLUSION: We found a strong correlation (R (2) = 0.91) between DNA damage in terms of tail length and malathion concentration in blood. Intensive efforts and trainings are thus required to build awareness about safety practices and to change industrial workers' attitude to prevent harmful environmental and anthropogenic effects.